Description
Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI) (By similarity).
Family
Belongs to the XPF family.
Species
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Sequence
MSIPSDAKQLYAEFLQQEVNQSTSAHQEKLVMVLNRALFALKNYPDPIYHPKDLLKVKGIGQTTMNKLSKRLKTYCEENGYSFPEESSSTDNTQSTQNDSNPSNTQKTRVRTLEPEENESQGTRKRRKKKYIPRNRSGGYGILLGLLELGCDKDGTACTRRQLIAVASKYCDQSYEKNPSTKEFYSAWSAIKSLKTNDLVIEQGRPSQFSLTEAGTILADSLKTANNIEFDVSSVYERRLNRGNTQNSFTNDEHDHTVNFSGLMNHANMSINESANSSRLFLDATANSSRIEQNEEPEVSAEISTPIPKQKVSKGRWKGVKYELWKPDSYDIILHIDHREVRSKEDRGFFARKLLQRGIETESSSLTVGDMIWLAKHKQSGQQCALDFIVERKRLDDLVISIRDNRFSEQKNRLQKTGCKHIFYLVEETTGYNVSDSADMMKTSIWTTVIYNDFHIKRTRNADTTVQWLTDMSLIIKELYSRKSLVVINHDHITNQSIYLTSLKMFRTEFERNKEIECCHNYESMQSAMVKTNLMTVKELYLRALMSVKGISLEKALMIQSRYPTFKTLLKAYRRCAAEADAKTLIQNELKDAPGNRKIGKSLSHTLWETFGKL
Simulated SDS-PAGE

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service