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Protein expression services for CHIT5 | Class V chitinase CHIT5

Description
Possesses chitinase activity in vitro toward glycol chitin, carboxymethyl-chitin, colloidal chitin, and the chitin oligosaccharides (N-acetylglucosamine) (GlcNAc)6 and (GlcNAc)5 (PubMed:27383628). Hydrolyzes (GlcNAc)6 into (GlcNAc)4 and (GlcNAc)2, or two (GlcNAc)3 molecules (PubMed:27383628). Has the capacity to inhibit hyphal growth of the fungus Trichoderma viride in an agar-plate bioassay (PubMed:27383628). Involved in symbiotic signaling (PubMed:30284535). Required for root hair infection threads (ITs) elongation and nodule development (PubMed:30284535). Possesses Nod factor (NF) hydrolase activity (PubMed:30284535). NFs are lipo-chitooligosaccharide signaling molecules produced by nitrogen-fixing rhizobia to initiate nodulation (symbiosis) on the roots of legumes (PubMed:30284535). Modulates NF levels and signaling to complete transition of infected nodules to functional nitrogen-fixing organs (PubMed:30284535).
Family
Belongs to the glycosyl hydrolase 18 family. Chitinase class V subfamily.
Species
Lotus japonicus
Length
397 amino acids
Sequence
MIIKLLVALIHYLHETMAVQSIITTPLLVILMSLRSYAFTEPSLHRQQPPSKGGVRAAYWPAWSDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDGIHNIRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDEKDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYLDWASPMCFDYNGAWANETGFNAALYDPNSNISTKYGIGSWIGSGVPAEKLVMGLPLYGRAWELKDPNDHGVGAKAVGPAVDTDGSMDYDEILVFNKDTGAKVVYDEVAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDWTISKQASNAWGY
Mass
44.4 kDa
Simulated SDS-PAGE
Western blot of CHIT5 recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make CHIT5 using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

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