Description
Catalyzes the reversible phosphorolysis of cellobionic acid (4-O-beta-D-glucopyranosyl-D-gluconate), a probable step in cellulose degradation. May be part of a metabolic pathway where cellobionic acid is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively. Produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity in the synthetic direction.
Family
Belongs to the glycosyl hydrolase 94 family. Cellobionic acid phosphorylase subfamily.
Species
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Sequence
MTRKMPTLVRPTHNGERYEITNPTAMPKAAGFLWNQKMMIQITCRGFATAQFMQPEPAKYAYAPNIEAKTFMQPEPNYYAHHPGRFVYIKDEETGRLFSAPYEPVRAPHDRFVFSAGKTDVFWVIESMGIRVEMTMGLPTHHVAELWTIKVKNLSSRPRKLSVTPYFPIGYMSWMNQSAEWNHNLNGIVASCVTPYQKAADYFKNKYLKDKTYFLCDVPPDSWEASQQAFEGEGGLHNPSALQERNLSGSDARYETPTAAVQYKIALGTGEQQEYRFLFGPAHDEAEIGAMRSKYLSKEGFEQTAADYAAYMARGRGCLHVETPDKDLDNFINNWLPRQVYYHGDVNRLTTDPQTRNYLQDNMGMNYIKPEVSRRAFLTAIAQQEATGAMPDGILLVEGAELKYINQVPHTDHCVWLPVTLEAYLNETGDYSLLKEKVPSANGDKLTVFERFCRAMDWLLKSRDHRGLSYIAQGDWCDPMNMVGYKGKGVSGWLTLATAFSLNIWAKVCDHEGETDLAKRFREGADACNAAANEHLWDGEWFARGITDDNVVFGIKEDKEGRIWLNPQSWSILSGAASPEQIDKMLPQIDSHLNTPYGIQMFGPPYTKMREDVGRVTQKAIGSAENAAVYNHAGIFFIHSLYELGAQQDRAFTLLRQMLPGPTDTDYIQRGQLPIYIPNYYRGAWKECPRTAGRSSQLFNTGTVSWVYRCIIEGLCGLRGDGEGLLIRPQLPSSWNSMKVTREFRGATFNVDIRRGNVKEVTVRNGDKVLPAPHVKDIEPGQTYNLTVTIP
Simulated SDS-PAGE

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service