Try expressing BCAT2 in cell-free | Branched-chain amino acid aminotransferase 2, chloroplastic
Description
Converts 2-oxo acids to branched-chain amino acids. Shows no kinetic preferences corresponding to anabolic or catabolic functions, but likely involved in BCAA biosynthesis.
Family
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
Species
Humulus lupulus
Length
408 amino acids
Sequence
MDCAAALLPGFHPNYLLCPSRHFSSLLPKTDLSSPLKFQLQNKQLSLASSHGFSPVICNATLSDTYSETVELADIDWDNLGFGFLPTDYMYNMKCAQGESFSNGELQRFGNIELSPSAGVLNYGQGLFEGLKAYRKEDGNILLFRPEENALRMRLGAERMCMPSPTVDQFVDAVKATVLANKRWIPPVGKGSLYIRPLLMGSGAVLGLAPAPEYTFLIYVSPVGNYFKEGVAPIHLIVEDNLHRATPGGTGGVKTIGNYAAVLKAQSAAKEQGYSDVLYLDCVHKKYLEEVSSCNIFVVKGNLIFTPAIKGTILPGITRKSIIDVARTLGFQVEERLVHVDELLDADEVFCTGTAVVVSPVGSITYHGERVPYNEGGVGAVSQQLYSALTRLQMGFIKDNMNWTVELS
Mass
44.5 kDa
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)Safety
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Cell-Free protein synthesis
Have you tried producing BCAT2 in a cell-free protein expression systems? We have solved cell-free protein expression scale-up and purification challenges so that you can obtain up to low-milligram quantities of proteins in hours.
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