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Protein expression services for PA1538 | Baeyer-Villiger monooxygenase

Description
Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters or lactones using NADPH and/or NADH as an electron donor. Thus, can convert bicyclo[3.2.0]hept-2-en-6-one into the oxidative lactone products 2-oxabicyclo[3.3.0]oct-6-en-3-one and 3-oxabicyclo[3.3.0]oct-6-en-2-one. Is also able to catalyze the sulfoxidation of methyl phenyl sulfide (thioanisole).
Family
Belongs to the FAD-binding monooxygenase family.
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Length
527 amino acids
Sequence
MYTPANNHNRSLAMSTQPTPAAARHCKVAIIGTGFSGLGMAIRLRQEGEDDFLIFEKDAGVGGTWRVNNYPGCACDVQSHVYSFSFEANPEWTRMFARQPEIRAYLEKCWEKYRLQEKTLLNTEIGKLAWDERQSLWHLHDAQGNHYTANAVVSGMGGLSTPAYPRLDGLENFQGKVFHSQQWDHDYDLKGKRVAVIGTGASAIQFVPEIQPLVAALDLYQRTPPWILPKPDRAISETERRRFRRFPLVQKLWRGGLYSLLEGRVLGFTFAPQVMKLVQRLAIRHIHKQIKDPELRRKVTPDYTIGCKRILMSHNYYPALAAANSTVITEGIRAVTANGIVDGNGREREVDAIIFGTGFTANDPIPRGVVFGRDGRDLLDSWSKGPEAYKGTTTAGFPNLFFLMGPNTGLGHNSMVYMIESQIAYVLDALKLMKRRELLSLEVKAPVQERYNEYLQRKLDRSVWSVGGCKSWYLHPVSGRNCTLWPGFTWRFRALTRQFDASAYHLTTTPLAALSNEARQQAEGVPA
Mass
59.5 kDa
Simulated SDS-PAGE
Western blot of PA1538 recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make PA1538 using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

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