Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).

Belongs to the GatB/GatE family. GatB subfamily.

476 amino acids

MNFETIIGLEVHVELKTNSKIFSPSTNEFGSDPNTNVNPIDLGYPGTLPVLNEEAVNFAMKAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDQPIGENGWIEIEVNGEKKKIGITRLHLEEDAGKLTHGEDGYSYVDFNRQGTPLIEIVSEPDMRSPEEAYAYLEKLKNIIQYTGVSDVKMQEGSLRCDANISLRPIGQEEFGTKAELKNLNSFSYVQKGLEFEEKRQEKELLSGGEILQETRRYDEKTKETILMRVKEGSDDYRYFPEPDLVPLYIDEEWKERVRSEIPELPDARLERYINELGLSEYDANVLTASKQMSDFFEEATAEGADMKQVSNWLMGEVSAYMNKHYKELDELAITPEALAKMINLIEDGTISSKIAKKVFAELVENGGDPQKIVEEKGLVQISDPAQLKQIVNEVLDQNEQSIIDFKNGKNKAKGFLVGQIMKATKGQANPPLVNKILAEELNNR

53.9 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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