Description
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Family
Belongs to the GatB/GatE family. GatB subfamily.
Species
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / JCM 1002 / NBRC 13953 / NCIMB 11778)
Sequence
MNFTSTIGLEVHFELKTKSKIFSPSPVTYGAKPNTETNVIDWGYPGTLPMVNKEVYRLGLMVALATHSHVNPVTHFDRKNYFYPDNPKAYQITQFFKPLAENGYVEVEVHGKKKKIHIHEMHIEEDAGKNTHGTNGFSYVDYNRQGVPLLEVVSEPEMTDPDEAVAYLEKLREIVQFTGASDVKMEEGSMRIDTNISIRPAGQKELGTKVEMKNLNSFEHVRMSLAYEQKRQQEILLSGGRVRLSTRRFDTNTGKTVLERVKEGDADYRYFPEPDLPPYHIKQEWVDEIAANLPKTADERRKIYVDEYGLKPYDANVLLQNKESSDFFDATVAAGADPQQAANWMNTQVNGYLNEKHAELKDIALTPENLAAMIKLISDGTISSKIAKKVFAETVANGTDPKKYVEENGMAQLSDESVLAPMVKEVVDANPQSVEDYKNGKDRAIGFLVGQIMKQTRGKANPKVINKLLLADLASR
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service