Description
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Family
Belongs to the GatB/GatE family. GatB subfamily.
Species
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Sequence
MTYQAVIGLEVHLQLNTRSKIFSACPADYHGAGPNEFTDPLTLGLPGTLPTLNRRAVELAMMFGLGLGCDVSGFTQFHRKNYFYPDAPKNFQLSQYDRPIARDGYLDVPGEGGPERIRIKRAHLEDDAGKLVHPTYAPYSLLDLNRAGSALIEMVTEADITGPEQARAFLESVQAIAQSLGVSDATPEEGKMRCDVNISIHKPGEPWGTKVEVKNLNSFRSVARAIEYEAARQAKVLDAGGIITQDTLGWDEGGQKTFLMRTKEGEADYRYFPEPDLPPLDITPEWIAEVRARMPELPAQKLERYRAAGVRESDAQTLSLSVSLSKFYDEALKSGSDTQKPDAQKLANWLLTDVAGALAAQEKGVEDSDLQPAHLAALVGLIDAGTISGKIAKDLLPDVLAGHDPAQLVQERGLSVVTDTGAIDAAIDAAMEADPATVEKVRGGNAKAMNALFGPVMKAMGGKAKPEVVRERLTAKLGL
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service