About Products Protein Database Contact

Try expressing rsgI3 in cell-free | Anti-sigma-I factor RsgI3

Description
Anti-sigma factor for SigI3. Negatively regulates SigI3 activity through direct interaction. Binding of the polysaccharide substrate to the extracellular C-terminal sensing domain of RsgI3 may induce a conformational change in its N-terminal cytoplasmic region, leading to the release and activation of SigI3.
Species
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)
Length
644 amino acids
Sequence
MDNIGVIIKIEGNEAIVMTDDCSFKKVPIKDGMHPGQKILVPNNEVIQKENKSIKRISAVATGIAAVFLMVLSLIWINKPGRPDGIYAYIDVDINPSLNFLIDREGKVKALNPLNDDAQEIIRGVEFEDMFFSEALTQIIKISKAKGIIDENKTNYVLICAALDDNYNLQSDDKSRAQTEFEEFLDGIRESIEKACGNTVIPQTVKVPFEYLKMAKQNDVSMGRYLVYQKLEDIGVNLSIEELKSLDIDEILKKYGVGFDELFKSEYTELPYGTLQTGEDSVVSTEDVPVSPKNAFETMAVPTNTPSISTKPSATPAENPTPKLTQKPTPVPAKTGERTSTTPTPTPAPTVRNGTGSGLRGEYYNNMDFSRFQFVRIDPCIDFDWGEGTPDQSIGKDTYSVRWTGKVEPRYSETYTFYTVTDDGVRLWVDGVLLIDKWKSQSATEHSEQIYLEAGKKYDIKMEYYQHVRAASAKLMWSSKSQQKEIIPSSQLYPSDGPLPQKDVNGLSAEYYGDAELKDKRFTRIDDAINFNWDKDFPVGELKDGKFSVRWVGKIDTRYTEEYTFHTVANGGVRVWINNVLIIDNWQNQGKEAENSGKIELKAGRQYDIKVEYCNYGEPAFIKLLWSSQRQKKEVVPSKNLFAD
Mass
72.7 kDa
Simulated SDS-PAGE
Western blot of rsgI3 recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Cell-Free protein synthesis
Have you tried producing rsgI3 in a cell-free protein expression systems? We have solved cell-free protein expression scale-up and purification challenges so that you can obtain up to low-milligram quantities of proteins in hours.

Order Here