Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.

Belongs to the class-II aminoacyl-tRNA synthetase family.

961 amino acids

MASNLQSQPKWTSKLVRDTFLQYFQGKGHTFVASSPVAPLSDPTLLFTNAGMNQFKSIFLGTVNPNSDFAQLKSAVNSQKCIRAGGKHNDLDDVGKDSYHHTFFEMLGNWSFGDYFKKEAIQYSWELLTQVYGLDPGRLYVTYFEGNKEGGLQPDLEAKALWKAVGVPEDHILPGNMKDNFWEMGDQGPCGPCSEIHYDRIGGRNAASLVNQDDPNVLEIWNNVFIQYNRENDGSLRSLPNKHVDTGMGFERLVSVLQDKSSNYDTDVFGPIFQTIQVITGAREYQGRFGTDDSDGIDTAYRVVADHVRTLMFAISDGVVPNNEGRGYVIRRVLRRGARYARKYFNVEIGSFFSKIVPTVVEQLGDMFPELKQKQQDVIEILNEEEISFAKTLDRGERQFEQYAQQAKTAGDHKLHGADVWRLYDTFGFPVDLTRIMAEERGLEIDDRQFEEARHKAKEASKGQKKTTTGSVKLDVHDLGKLEKMNGVPKTDDSAKFEIGNVTAQIKAIYHGKTFHSSTQNLDDSEQIGIILDRTNFYAEQGGQENDTGRIVIDGQAELEVVDVQQYAGYVLHTGYMKYGSFKVDDTVICEYDELRRWPIRNNHTGTHILNFALRGVLGDGIEQKGSLVSAEKLRFDFSHKSPVTDVELVKIEDLSTQYIRRNCLVYSKEVPLSIARNIAGVRAVFGETYPDPVRVVSVGAELDEILKNVEDPRWADFSIEFCGGTHVQKTGDIKDLVILEESGIAKGIRRIIAVTGEDAHEVQRLAEEFEKRLNHLEAMPSGSAKEQNAKQIQVELNQLSISAVQKSRFRERFTQINKKIIDEQKAQQKLESKKAVESITSYFAAPENANKSWLIAKLPISANAKAVSECLTYVKTKLADKMVYVLASSLEDGRVAHGCYVPKAISDQGASASEWSSAVSRAVGGKAGGKGPTSIGNGINPEKVDDAVDLASEYLSKFKL

107.3 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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