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Try expressing apc5 in cell-free | Acetophenone carboxylase epsilon subunit

Catalyzes the carboxylation of acetophenone to form 3-oxo-3-phenylpropanoate (benzoylacetate) in the anaerobic catabolism of ethylbenzene. Also carboxylates propiophenone at the same rate and 4-acetyl-pyridine at lower rates.
Aromatoleum aromaticum (strain EbN1)
303 amino acids
32.1 kDa
Simulated SDS-PAGE
Western blot of apc5 recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
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Cell-Free protein synthesis
Have you tried producing apc5 in a cell-free protein expression systems? We have solved cell-free protein expression scale-up and purification challenges so that you can obtain up to low-milligram quantities of proteins in hours.

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