Protein expression services for addA | ATP-dependent helicase/nuclease subunit A
Description
The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.
Family
Belongs to the helicase family. AddA subfamily.
Species
Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / JCM 1002 / NBRC 13953 / NCIMB 11778)
Length
1227 amino acids
Sequence
MAVKYTPDQARAIESRGQDLLVSASAGSGKTSVLVERVIREIMDDHLEVNQLLVITFTRAAASEMKQRIKQRLQDRLQEETDASQADFLRRQLAEIDTAIISTIDSFCLDVIRRFYFAIDIDPDFSILTDATQIELLKERALRDVENGYLQNEDQAKKDRFLALYDAFAGDSNANSARDLLLDLYQFAMARPNYRAWLQKLAAPYQLETSDVVDSALWQEKIKPYLEEEFSNLADKLTALMAEADFDHEKFAKYQPAFTAFATSLASYRESLATDDPFDRQRELLAACQFDGTLRTNKEIADFVEEAKEVKEAGKQLVFNVYTGFYASSNADQQALLAKGAEIASAAAEVELAFIDRFNELKRADRVLDFSDMEQLAYEILTQDSSNSDLARAYYQSRFKEIMVDEYQDTNALQDGLIQRLKKAGKNNLFMVGDVKQSIYGFRQAEPSLFIAKYDEYGQENSAGKQRIIFAENFRSSQPVTQAVNLIFDSLLTKDFGGIDYQKEGQLKFAAGYDPEAALPTETEVLYQEDSSATDDDGELNQGDLAMVISRIQKLIADQTPIFDPKTGQTRPVSYGDIAILTRSKTSNLDIKQEFDRYGVPLFVMDVQNYFQTFELTVIMSYLKIIDNPDQDIPLVAVLRSPIFNFSSSDLAEIRLVNKSVSFYAALRTYAKKDTDLAARCRDFLAQLQDLRDFSLSHRISELLWTIYERTSFLEIVTAMTNGQQRRLNLTALYERASAYESSGFKDLYQFINFIARMRKNQKDLAQPILSENAGNSVKLMTIHASKGLEFPVVFVLGLEHRYNYQQDITGSYVLDASGLGLSFAYPFDEAEYRADTLANVWLKIAKKQKLLEEEARLLYVALTRAKQKLILAANIKLPARTDLAGLEEKWAKEISAGRLTLLDKMKVAKPLDFLAPALARAKQVKRLGEKAVSDLATGQEGSLVFVHFDPKKDQAQLPDSEAVAASGADLTEDEAAVFKQAEKLYTFSQGGYPYLDASRTTAYQAVSEIKKVFGDPIEDELADSHISELQSANRYLQPIDTEPDFLFQNTVSSAELGTASHLVLQYYDYAKGDKNAIDSCIAGLVEKGRLSQTLASMLDREALSWFVKSDFAKDFYQQPDRLHREENFATILSPKTLFKDFSDFPGKILVHGTIDGYYEAENGIILFDYKTDHVNPRKQEEAIQKLKEKYQGQLRLYERALNESGRLPVLKKYLVLLSCREIVEVD
Mass
138.8 kDa
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)Safety
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Protein synthesis service
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