Protein expression services for addA | ATP-dependent helicase/nuclease subunit A
Description
The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the L.lactis chi site (5'-GCGCGTG-3'), which stimulates homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has 3'->5' exonuclease activity and probably also performs the helicase function.
Family
Belongs to the helicase family. AddA subfamily.
Species
Lactococcus lactis subsp. cremoris (strain MG1363)
Length
1203 amino acids
Sequence
MSEVKLTPEQNEAIHSSGKNILVSASAGSGKTFVMAQRIVEKVKQGIEIDRLFISTFTKKAASELRMRLERDLKKARQESSDDEEAHRLTLALQNLSNADIGTMDSFTQKLTKANFNRVNIDPNFRILADQTESDLIRQEVFEQLVESYLSADESLNISKDKFEKLIKNFSKDRNILGFQKVVYTIYRFASATENPISWLENQFLKGFETYKSLTDLSEDFTVNVKENLLTFFELLENSLTNGVIAKKGAGRDKANLILDNKNELLEAISKKDFVTCTALFLSIDTDIRVGSSKDEALSALKKDFSAQKQDLVGSKSKPGELRKFVDKIKHGQLIEKYQNQAFEIASDLQKFIIDFYKTYLERKKNENAFEYSDIAHFAIEILEENPDIRENLREHYDEIMIDEYQDTSHTQERMLELLSNGHNLFMVGDIKQSIYGFRLADPGLFLEKYKSYDQAENPNQLIRLKENFRSRGEVLNFTNDIFKHLMDEKLGEMTYGKEEALVQGNISDYPVEAEKDFYPELLLYKENTSEEEIEDSEVKISDGEIKGAAQEIKKLIEYGVEPKDIAILVRSKSNNNKIEDILLSYDIPVVLDEGRVDFLKSMEVLIMLDVLRAIDNPLYDLSLVAMLRSPLFGFNEDELTRISVQGSRDLRFWDKILLSLKKEGKNPELINLSLEQKLKAFNQKFTEWRKLVNKIPIHRLLWKIYTETYYFDYVGALKNGEMRQANLQALSVRAESYESSGYKGLFKFVRLINKFMEQNNDLASVNIKLPQNAVRVMTFHKSKGLEFDYVFLMNLQSRFNDRDLKEDVILSREHGLGMKYIADLKAEPDVITDFPYALVKMETFPYMVNKDLKQRAALSEEMRVLYVAFTRAKKKLYLVGKIKDTDKKAGLELYDAATLEGKILSDKFRNSSRGFQHWILALQNATKLPMKLNVYTKDELETEKLEFTSQPDFKKLVEESEKFDNIMSFSDEIKEAQKIMNYQYPHQAATELSSIQTPSQVKKRSYEKQLQVGEVQPVSEFVRVKNLDFSDFGPKKITAAEMGSATHSFMQYADFSQADLFSFQATLDEMGFDEKIKNQIDITKILTLFDTEFGQFLSENVDKTVKEAPFSMLRTDEFAKEQYIVRGICDGFVKLADKIILFDYKTDRFTNVSAISEIKERYKDQMNLYSEALQKAYHVNQIDKYLILLGGPRKVFVEKIDD
Mass
138.9 kDa
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)Safety
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Protein synthesis service
Make addA using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)
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