DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks. Helicase with 3'-to 5'- polarity; able to unwind over 100 bp of DNA at 50 degrees Celsius. Unwinds forked DNA, preferentially on lagging strand forks; has weaker activity on Holliday junctions. Displaces the invading strand in DNA D-loops. Unwinds short oligonucleotides from dsDNA with 3'- but not blunt ends or 5'-ssDNA tails in an ATP-dependent manner. ATPase activity is stimulated by ssDNA but not dsDNA, protein binds ssDNA, dsDNA with 5'- or 3'-overhangs but not blunt ended dsDNA and replication forks. Replication forks bind both this protein and RPA. RPA does not stimulate the helicase activity of this protein.

Belongs to the helicase family. Hel308 subfamily.

690 amino acids

MKSLPPEMRQILGDCYPHIRELNPAQRSAIEAGYLESEDNYIIAIPTASGKTLLGIIAALKTVMEGGRVIYTVPLLSIQNEKIKEFRKLEEHGIRVGKDPRTSDIAVMVFESFDSLTRFSWNILREVDLLIVDEFHMIGEYTRGPVIESAITRARTLNPSVRIVALSATLSNMDEIAGWLDARVVEHDYRPVPLHREVLDTEMFGVREKNDVVLKVLERSLEDGSQTLAFVSTRRFTESLASHLADKISGKIPDDMVESFREVAGKVLEVPKSRGSPPTSTCLKLAECLEAGIAFHHAGLFNRQREIIEDEFRDGNILMITATPSLMYGVNLPSRTVVIRDYTRWTSQGPRRIPVFDYEQMSGRAGRPQYDDAGYSYLIARSHDEAMDLEEYYIRGEVERTTSRIIENRDALYRQIIAQVASGLSGTTEELADFFRNTFYGYQMVEGPFSDSFGMDSIQYEVENATEYLMRNRILYPGPEGFSATEFGLLIAKSNYSVETAIKLHQFASEMDEMDIYRLIYEITRTPDMPLISFKGRKSRDPVRDKLMEHGLFLMDVGNEEATAAALIEWINERTEYEIENAFHVYAASTRRSAYEASKIVKFFGKICEIMGVYRHSSQLEILSARLYYGVKEDAIPLVVGVRGLGRVRARKIIKTFGEDLRHVREDELKRIDGIGPKMAGAIRRYCERF

78.5 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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