An ATP, Mg(2+)-dependent DNA 3'-5' and 5'-3' helicase that may be involved in repair of stalled replication forks. Stimulated by both ss and dsDNA. Unwinds both leading and lagging strands in replication fork structures, unlike orthologs in P. furiosus and M. thermautotrophicus which only unwind the lagging strand and only have 3'-5' helicase activity. Preferentially binds dsDNA with overhangs or branched DNA. Able to anneal DNA substrates that could form a replication fork-like structure, has replication fork reversal activity (at least in vitro).

Belongs to the helicase family. Hel308 subfamily.

704 amino acids

METISIDDLPLDIKIIDILKRRGIRTLNPPQSEAIRKGLLDGKRLLVTSPTASGKTLIAELGMINYLLSKGGKAIYITPLRALTNEKYNTFKDWETLGIKTGMTSGDYDTDDAWLENYDIIVTTYEKLDSLWRHKAKWLNEVSYFVLDEFHYLNDPERGPTVESVAIRAKKRGIVLGLSATISNGKEIANWLNAELVATNWRPVPLKEGIIYPEKKGFVVVYKDNTSRKVYGDDAIIAYTLDIVSKGGQVLVFRSSRKLAENTARKIVQYMNFVKLEDKKLLEIARKIKEVEDAGSNEKEDLYNLVLRGVAYHHAGLSKGLRDIIESSFRDRILKVIVATPTLAAGVNLPARAVVIGDIYRYNRKVVGYMDLIPVMDYKQMSGRAGRPGFDENGEAVVVVRNKREAEKVYERYLMSDVEPIESKLGSESAFYSFLISIIASEGEKTTEELMEYVKETLLPKELAKKYFRSGLDWLLQHDIFAEISDKITLTRFGRRISDLYINPFTAVTIREALEKNEKGCEIAYLHLLAYTPDGPSIGVSRAEEDALIDELNCELFVDEPEDEYEFSNYISALKVAYIVYDWVNEIDEDTILGKYGIGSGDLRAIIDTMDWLTYSGYHVASVLELKDHKDILEELHARVKDGVKPELIELVKIPGIGRVRARLLYQHDIKKPEDIVLNPEKVKQLLGPNLGEKIVREAARTIA

79.7 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.

Make hel308 using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)

Order Here