Catalyzes the conversion of aminoimidazole ribotide (AIR) to 5-hydroxybenzimidazole (5-HBI) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction. Is thus involved in the anaerobic biosynthesis of the benzimidazole lower axial ligand of the cobamide produced by G.metallireducens.

Belongs to the ThiC family. 5-hydroxybenzimidazole synthase subfamily.

435 amino acids

MKTQIEIAREGTISSQMMAVAAEEHVSPDYVRQMVAEGKIVIPGNHSRKPRAVGIGKGLRTKVNASIGTSSDIIDYGAEVRKALAAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGNVPLYQAFCEAARKYGDPNKLDPEMLFDLIEKQCEDGMAFMAVHCGINLYTIERLKRQGYRYGGLVSKGGVSMVAWMMANRRENPLYEQFDRVTSILRKYDTVLSLGNGLRAGAIHDSSDRAQIQELLINCELAELGREMGCQMLVEGPGHVPLDEVEGNIQLQKRMSGGAPYYMLGPISTDVAPGFDHITAAIGAAQSSRYGADLICYITPAEHLALPNEEDVRQGVKAAKIAAYIGDMNKYPERGRERDKEMSKARRDLDWKKQFELALFPEDAKAIRASRTPEDEATCTMCGDFCASRGAGKLFAADLRGDKI

47.7 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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