Involved in the ethylmalonyl-CoA pathway, a new acetyl-CoA assimilation strategy that operates in a number of bacteria and replaces the glyoxylate cycle. Catalyzes the oxidation of (2S)-methylsuccinyl-CoA to yield mesaconyl-(C1)-CoA. Highly specific for (S)-methylsuccinyl-CoA.

Belongs to the acyl-CoA dehydrogenase family.

548 amino acids

MTGQPLLGDLLTLASDALPEVEALFETARSALKERVTTDGKVSSKALEEEQFAAHALSWLATYVESLRQMRAWAGRLETEGRFGEMEALILQIAFGEYLAQIRGGIPMSQTETARVQDIGIELGHPGEAVRRLIQAGNTPAARARLVALMRDNHGRATFGASGLDEELEMIRDQFRRFADERVAPHAHGWHMRDELIPMEIVEALAEMGVFGLTIPEEFGGFGLSKASMVVVSEELSRGYIGVGSLGTRSEIAAELILCGGTDAQKAAWLPKLASGEILPTAVFTEPNTGSDLGSLRTRAVKDGDEWVVHGNKTWITHAARTHVMTLLARTDLETTDYRGLSMFLAEKVPGTDADPFPTPGMTGGEIEVLGYRGMKEYEIGFDGFRVKAENLLGGVEGQGFKQLMQTFESARIQTAARAIGVAQNALEVGMQYAEERKQFGKALIEFPRVAGKLAMMAVEIMVARQLTYHSAWEKDHGQRCDLEAGMAKLLGARVAWAAADNALQIHGGNGFALEYQISRILCDARILNIFEGAAEIQAQVIARRLLD

59.8 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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