Specifically cleaves olefinic bonds in alpha,beta-unsaturated carbonyls and may be involved in detoxification or modification of these reactive compounds (Probable). May be involved in the biosynthesis or metabolism of oxylipin signaling molecules (PubMed:10872231) (Probable). In vitro, reduces 9R,13R-12-oxophytodienoic acid (9R,13R-OPDA) to 9R,13R-OPC-8:0, but only poorly 9S,13S-OPDA, the natural precursor of jasmonic acid (JA) (PubMed:10872231). Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro and in vivo by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT) (PubMed:19605548). Functions in an alternative and OPR3-independent pathway for JA biosynthesis (PubMed:29291349). Catalyzes the NADPH-dependent reduction of 4,5-didehydrojasmonates to jasmonates (PubMed:29291349).

Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.

374 amino acids

MEMVNAEAKQSVPLLTPYKMGRFNLSHRVVLAPLTRQKSYGSVPQPHAILYYSQRTSPGGFLIAEATGVSDTAQGYPDTPGIWTKEHVEAWKPIVDAVHAKGGIFFCQIWHVGRVSNRGFQPRRQAPISCTGKPIMPQMRANGIDEARFTPPRRLSIEEIPGIVNDFRLAARNAMEAGFDGVEIHGAHGYLIDQFMKDKVNDRTDEYGGSLQNRCKFALEVVDAVAKEIGPDRVGIRLSPFADYMESGDTNPEALGLYMVESLNKYGILYCHMIEPRMKTVGEIAACSHTLMPMREAFKGTFISAGGFTREDGNEAVAKGRTDLVAYGRWFLANPDLPKRFQLDAPLNKYNRSTFYTSDPVVGYTDYPSLESTA

41.6 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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