Protein
Dual specificity protein kinase zakA
Organism
Dictyostelium discoideum
Function
Positive regulator of gsk3/gskA activity required for cell pattern formation and a downstream effector of carC. The kinases, gsk3/gskA, zakA and zak2, form part of a signaling pathway that responds to extracellular cyclic AMP. The pathway has a role in transcriptional regulation; required to direct prespore/spore fates during development. ZakA negatively regulates prestalk differentiation by regulating expression of ecmB. Phosphorylates Y-214 of gsk3/gskA, in vitro.
Similarity
In the C-terminal section; belongs to the protein kinase superfamily. TKL Tyr protein kinase family.
Sequence
MHYHNKDDWEEISFIGEGQYGRVIKCRKKNGFILNEQVDYVAIKIISKDKFKRNETDILEKIRLFNIPRYYSHAEDDNFIYIYMEYIEGENLANILKTKKQGRFKESRIISMIADLVETLSFLHKHHVIHRDIKTANLVLDKNKNLKLIDFGASTIQNKKQFEQYLNETTNNNNNPNNNNNNNNNNNNNNNNNNNNNNNNNNINNINNNNDLNGSGSGISTYLNEQYKQSSFAIIGTFNYMAPEVKRNYRATRKSDVWSLGCTIIEMAGGDLSQKLNGIPIIPDHLSDTLKDFLNHCLVIDPKKRSYMEELLSHKLIVHIIGPNKSKNYGVEPKFKDDDDFEEIENEKDNYLSSRFPAKFAPQYEKPKWEIEFEELEFDKDDSEGGAGNFGDVKKGLLNETEVAIKFVKKAHCEAITVCDTFYHEVLILSNLRHPNIVQFMAACIKYGEKETNHCIVSEWMSGGNLTQFLMNNHKVLENNPHLRVKLLTDIAKGILYLHKQHIIHRDLTSNNVLLDFKREILPNQLYGSNEFTAKVCDFGLSSNQSESKKLRGGSIHYMAPENLNGSPINEKSDIYSFGLLVWQMFSYAPPNTIYSPKEMASMVSDPKQNYRPQIPFNVPLKFKELITQCWDRNPLNRPKDFSIIIEKLKEIGLTYNRSSSNVSPINSPLINNNNNNYNNNHLNSLSSSLNSSPTYYAKTFGDSNSNIDVYHSADSITPIVSSPPIIKIDLTQDDWDSKLKQLDLEHENKSLISISINDNNNNNINNNNTNNNNVNDLGYC