About Products Protein Database Contact

xanE

Gene
xanE
Protein
O-methyltransferase xanE
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
Length
431 amino acids
Function
O-methyltransferase; part of the gene cluster that mediates the biosynthesis of the isocyanide xanthocillin and its derivatives (PubMed:29844112). The first step of the pathway consists in the conversion of tyrosine into a vinyl-isonitrile intermediate by the isocyanide synthase xanB (PubMed:29844112). Subsequent oxidative dimerization of this intermediate to form xanthocillin may involve the cytochrome P450 monooxygenase xanG, whose expression is coregulated with that of XanB (PubMed:29844112). Xanthocillin can be further modified by the isonitrile hydratase-like protein xanA which introduces N-formyl groups and the methyltransferase xanE which introduces methyl groups, leading to the production of several derivatives including fumiformamide (PubMed:29844112). Finally, fumiformamide can be subject to both oxidative and reductive cyclization to yield melanocins E and F, respectively (PubMed:29844112).
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.
Mass
47.75 kDa
Sequence
MSTQHGPKSTRTNDLYELAVNISSTVEAFVGRLDAIGAERPNLDNPFPEIIQDEGAQLARLKILRLCERLMALVQGPVQWLMFQNMAFLDAACVGAILDMGIHDIIAPGPEPTSLDQIVEATGASKDILKRIMRVCTQRLVFDEIAPEQFIHNGVSLQFLAPPVQALISHACDDGLRMAAHFTDSLKKTDWKGSDDPENTAFSLAFNTNKGLFDYFYTEDLARGQRFALGMAGSEIMKTLTEDMFPFESLPQGAKVVDVGGGRGHVSVRIAEKVPGLNFVVQDDESMLEAGQAEGVPKAVKDRIEFMPHDFFNEQPVKGADVYLLRFILHDHSDSRCAKILSNIVDAMEPGKSRILIDDAIVPSFLGPESSRFFNLLDLYMLAGLNGKERTLEQWNYLIQMVSPKLVLEKVWKTPNGGPESGTILELRLQE