Protein
G1-specific transcriptional repressor WHI5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Function
Transcriptional repressor that negatively regulates G1-specific, SBF- and MBF-dependent transcription. Binds via SBF to promoters of G1-specific genes to repress transcription in early G1. During G1, phosphorylated by cyclin-CDK CLN3-CDC28 and progressively hyperphosphorylated by CDK associated with other G1-cyclins, which leads to dissociation of WHI5 from SBF, activating SBF-dependent transcription in late G1. Elimination of CDK activity at the end of mitosis by the mitotic exit network (MEN) allows WHI5 to reassociate with SBF to again repress G1/S transcription.
Similarity
Belongs to the WHI5/NRM1 family.
Sequence
MSLRTPKRSRTSDEQEQEQEQEQVQNPDTHVNNEHQQRPGPTTLLSTPVRLKNGFGTPSPPSPPGITKSITKSRRRPSTTSLQGIFMSPVNKRRVGITAHGRVYDHNDDGHESESEDDENEEENENQKKYDGHVSMPLLPPTTPKSRRSEVFLSPSPRLRSPPTAARRSTGERPIREISHTLRTRLNYALVKLQNGWTDKTLPELETELAPAVQTPPRRYHNRFPDSADAGTSAHTAFLQALGGHPPREEATAVETLMLLSSPTKKQQHRPVPATSAGEPTDETEPESDTEVETS