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wapI

Gene
wapI
Protein
Immunity protein WapI
Organism
Bacillus subtilis subsp. spizizenii (strain TU-B-10)
Length
306 amino acids
Function
Immunity protein component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. Neutralizes the tRNase activity of cognate toxin WapA upon expression in E.coli. Does not inhibit WapA from other strains of B.subtilis. The WapA C-terminus cannot be expressed on its own in E.coli, however it can be cloned in the presence of its cognate immunity protein gene. Cell contact is probably necessary for growth inhibition.
Mass
35.616 kDa
Sequence
MILSSFLKIERMSSMHELKFRDLNVEVNEEDTVLLSMTESEIIVLTKKEIDYGAKYINHIVLYCNTDGRFLNSFSIQTNEQVINVQKVDDSFLFLIDKEYEDSVRDVEPNIYLWNPIEGFHQSFYAGRYINSMIIDQNKNLWVGYDETGIFSCVDQEISTRGINKFVLKNGKYELYFHGVSSYVIDQYFSTFVSEDAIYLYYRSMGEDYLQKLNLLGETLERVEVGIECSSCIKNGSSIYLFSRDDDSYNIEKVFKTNDMQNYVEQKISNENNGESLCFTQVASYKDKVAGIDHNNKLFLLNNQSL

Gene
wapI
Protein
Immunity protein WapI
Organism
Bacillus subtilis (strain 168)
Length
142 amino acids
Function
Immunity protein component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. Neutralizes the tRNase activity of cognate toxin WapA upon expression in E.coli. Does not inhibit WapA from other strains of B.subtilis. The WapA C-terminus cannot be expressed on its own in E.coli, however it can be cloned in the presence of its cognate immunity protein gene. Cell contact is necessary for growth inhibition.
Mass
16.429 kDa
Sequence
MAKIKDDCIELELTPRRYQELDDDPFILSVFELLENKKAVVRDFSAVLLESEYKVLISGIETMIKGNQDSISLETIEPFLFLSIDQENGNYRIKIKIIFDDYKESKSNSNLFEINCNEEKLESFVTALKLNLENTKNPSKLP

Gene
wapI
Protein
Immunity protein WapI
Organism
Bacillus subtilis subsp. natto (strain BEST195)
Length
119 amino acids
Function
Immunity protein component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. Neutralizes the tRNase activity of cognate toxin WapA upon expression in E.coli. Does not inhibit WapA from other strains of B.subtilis. The WapA C-terminus cannot be expressed on its own in E.coli, however it can be cloned in the presence of its cognate immunity protein gene. Cell contact is probably necessary for growth inhibition.
Mass
14.709 kDa
Sequence
MKFFKRYNIDQKTLDEFKKYYVLLHGPFPNDMYDFEEETNTSLDEFYEFFALITGSLNYIIEDKKIPRYQREMLKKTFYEHYPHFRNYKSDILKYQELSECLEFHEKIRILINKLITGG