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vha-2

Gene
vha-2
Protein
V-type proton ATPase 16 kDa proteolipid subunit 2
Organism
Caenorhabditis briggsae
Length
161 amino acids
Function
Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Involved in necrotic cell death. Required along with other vacuolar ATPase components for the removal of protein aggregates which form in immature oocytes in the distal gonad. This removal occurs as the oocytes mature and move to the proximal gonad, is triggered by the introduction of sperm through mating and occurs before fertilization. The introduction of sperm triggers V-ATPase accumulation in proximal oocytes and induces lysosomal acidification which leads to engulfing of protein aggregates by lysosomes and subsequent clearance of the aggregates. Lysosomal acidification also leads to changes in mitochondrial morphology and function. Mitochondria in distal immature oocytes are fragmented, produce high levels of reactive oxygen species (ROS) and have high membrane potential, indicative of metabolic inactivity. In contrast, mitochondria in proximal mature oocytes are tubular with lower ROS levels and membrane potential, indicative of an active metabolic state required for aggregate mobilization before clearance.
Similarity
Belongs to the V-ATPase proteolipid subunit family.
Mass
16.402 kDa
Sequence
MSYDLETAEHAAYAPFFGYMGAASAQIFTVLGAAYGTAKSAVGICSMGVMRPELIMKSVIPVIMAGIIGIYGLVVAMVLKGKVQAASAGYDLNKGFAHLAAGLTCGLCGLGAGYAIGIVGDAGVRGTAQQPRLFVGMILILIFSEVLGLYGMIVALILGTS

Gene
vha-2
Protein
V-type proton ATPase 16 kDa proteolipid subunit 2
Organism
Caenorhabditis elegans
Length
161 amino acids
Function
Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase (By similarity). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells (By similarity). Involved in necrotic cell death (PubMed:22157748). Required along with other vacuolar ATPase components for the removal of protein aggregates which form in immature oocytes in the distal gonad (PubMed:29168500). This removal occurs as the oocytes mature and move to the proximal gonad, is triggered by the introduction of sperm through mating and occurs before fertilization (PubMed:29168500). The introduction of sperm triggers V-ATPase accumulation in proximal oocytes and induces lysosomal acidification which leads to engulfing of protein aggregates by lysosomes and subsequent clearance of the aggregates (PubMed:29168500). Lysosomal acidification also leads to changes in mitochondrial morphology and function (PubMed:29168500). Mitochondria in distal immature oocytes are fragmented, produce high levels of reactive oxygen species (ROS) and have high membrane potential, indicative of metabolic inactivity (PubMed:29168500). In contrast, mitochondria in proximal mature oocytes are tubular with lower ROS levels and membrane potential, indicative of an active metabolic state required for aggregate mobilization before clearance (PubMed:29168500).
Similarity
Belongs to the V-ATPase proteolipid subunit family.
Mass
16.41 kDa
Sequence
MSYDLETAERAAYAPFFGYMGAASAQIFTVLGAAYGTAKSAVGICSMGVMRPELIMKSVIPVIMAGIIGIYGLVVAMVLKGKVTSASAGYDLNKGFAHLAAGLTCGLCGLGAGYAIGIVGDAGVRGTAQQPRLFVGMILILIFSEVLGLYGMIVALILGTS