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vapC

Gene
vapC
Protein
Ribonuclease VapC
Organism
Rickettsia bellii (strain RML369-C)
Length
134 amino acids
Function
Toxic component of a type II toxin-antitoxin (TA) system. Has ssRNase activity. Its RNase activity is partially neutralized by cognate antitoxin VapB. Rapidly induces apoptosis upon microinjection into mouse fibroblasts (L929 line). Probably contributes to host cell death if bacterial cell lysis occurs during host infection.
Similarity
Belongs to the PINc/VapC protein family.
Mass
15.28 kDa
Sequence
MGLIVDTSIIIALERGKISTKAWSNYDQAYINPIVLTELLIGIDRVKDENKRGQCLTFIEYVKSLFTLLPFGIEEAYVYAKIIDNLYKERITIGVHDLLIAATAITYNYPILTLNTKDFKRIPELEVLTVPLKD

Gene
vapC
Protein
tRNA(fMet)-specific endonuclease VapC
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Length
132 amino acids
Function
Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity.
Similarity
Belongs to the PINc/VapC protein family.
Mass
14.938 kDa
Sequence
MLKFMLDTNTCIFTIKNKPEHIRERFNLNTSRMCISSITLMELIYGAEKSLAPERNLAVVEGFISRLEVLDYDTQAAIHTGQIRAELARKGTPVGPYDQMIAGHAGSRGLVVVTNNLREFERIPGIRIEDWC

Gene
vapC
Protein
tRNA(fMet)-specific endonuclease VapC
Organism
Shigella flexneri
Length
132 amino acids
Function
Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity.
Similarity
Belongs to the PINc/VapC protein family.
Mass
14.817 kDa
Sequence
MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Gene
vapC
Protein
Ribonuclease VapC
Organism
Haemophilus influenzae (strain 86-028NP)
Length
132 amino acids
Function
Toxic component of a type II toxin-antitoxin (TA) system (PubMed:27672196). Acts as an RNase (Probable). Its toxic effect is neutralized by cognate antitoxin VapB2 but not by non-cognate antitoxin VapB1 (PubMed:27672196).
Similarity
Belongs to the PINc/VapC protein family.
Mass
15.148 kDa
Sequence
MLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAVIEDFLSRLTILDYQPKHAAHFGNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Gene
vapC
Protein
Ribonuclease VapC
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Length
129 amino acids
Function
Toxic component of a type II toxin-antitoxin (TA) system. A sequence-specific endoribonuclease, cleavage occurs after the first AU in the consensus sequence AUA(U/A); RNA secondary structure is probably important in substrate choice. Cuts in 5' and 3' UTRs. The TA system acts as a post-transcriptional regulator of carbon metabolism; in M.smegmatis 3 TA systems (VapB-VapC, MazE-MazF and Phd-Doc) may be involved in monitoring the nutritional supply and physiological state of the cell, linking catabolic with anabolic reactions. When overexpressed inhibits cell growth, via translation; 10-fold in a wild-type strain, 100-fold in a vapB-vapC disruption mutant (PubMed:19445953). Overexpression of VapC leads to differential expression of 205 genes; many down-regulated genes are predicted to be involved in the transport and catabolism of carbohydrates, while genes involved in phosphate transport and scavenging are up-regulated. VapC is bacteriostatic, not bacteriocidal; cells are ovoid, non-replicating and have decreased transcription, becoming dormant (PubMed:24417293). Digests ssRNA but not tRNA, dsRNA, ssDNA or dsDNA. The VapB-VapC complex binds its own promoter DNA, and VapC alone binds RNA.
Similarity
Belongs to the PINc/VapC protein family.
Mass
13.91 kDa
Sequence
MVIDTSALVAILTDEPDAELLEGAVADDPVRTMSTASYLETAIVIESRFGEPGGRELDLWLHRASVALVAVDADQADAARLAYRRYGKGRHRAGLNYGDCFSYALAKVSGQPLLFKGEAFRLTDVAAVH