ufd2

Ubiquitin conjugation factor E4

1010 amino acids

E4 ubiquitin chain-elongation enzyme specifically involved in polyubiquitin chain assembly. Binds to cdc48 and elongates mono- and diubiquitinated ERAD substrates presented by the ufd1-npl4-cdc48 (UNC) AAA ATPase complex to a chain length of 4 to 6 ubiquitin moieties. Delivers these polyubiquitinated substrates to downstream ERAD components, which target them to the proteasome. Enhances ubiquitination at 'Lys-48', but not at 'Lys-29' of the Ub moiety.

Belongs to the ubiquitin conjugation factor E4 family.

115.276 kDa

MSDLEKIRLKRLAKLQQTNSEANSSKEPKESNIAPEPKKPDLKKRFIGSKATTSNSEQKEISPPVTSGAPKHRLFSKDEWMHFITCQALNITLSETDSSKYYLEGFKKDLEEEGSPLLFNENNVDSALLSRLSTTGNNTFSYLLQSWSFLYQYKKRLPKDENQDFKIHYLSLLKSLLVSYAGIVVMLPDTFNSETIDLAEVLIGAEGIPLEFLSEFVQRFEHENLDELFIPVLESLSLKIGLMNVDTVQMNVMQIILQLVSLKPIALLLPKLPSWNPTNNAGEIEYKTFLGRISSLSVFTQDVASRYFSNSTERSAQNISSSISSLKLTMSTYQDVLFQIFNTLIRTSTSLRESVLDFFAMVVNANHKRQSIQVNHFDITSDACMLNFSHVLSRLSEPFLDIGCSKIDRVQVEYFRRNPRVDIKEETKLNADQKASESFYSKPAEGSNNFISDIFFLNLAFHHYGVNATFKALEQLVQSIRDSEKLKERLETEQQNMSGSFQATRLTAQLSRLDQRLDLDRSFVHCYEIMLTQTSDTSRSFSFLNFVAIWLSRLADGQSSTYPKMPLSLPFNENAPEAFKCLPEYFIETITDYMLSLFKTSSSTLTLHSLEPLCEFCVSFLTQANYIKNPYLRAKLAEILYFGVQTHVGRSELLLDVVRTSKVATRWLLPALMAFYIEIESTGQSTQFYDKFNIRFYICEVFRTIWKQPAYFGKLEQEQKTNLPFFVKFVALMLNDATYLLDEALLKLTEIHNLQSLLADAISNSNSNQNVQESQSNLAAAERQASTYCQLGNETIFMLKLFTSSIPKAFCAVEIVDRLAAMLNYNLQALCGPKCSNLKVEDPTKYHFNAKTLLSIIFDVYLNLCNEPAFVEAVAHDGRSYSKEIFERATSIMTKHNLKSSFDIEAIKEFVNRVEAFRLQEATEEEDMGDIPDYFLDPLMFTIMKDPVVLPRSGISIDRSTIKAHLLSDATDPFNRTPLTLDDVTPNDTLREEINTFLKSKRNKHSRNSE

UFD2

E4 ubiquitin-protein ligase UFD2

961 amino acids

E4 ubiquitin chain-elongation enzyme specifically involved in polyubiquitin chain assembly. Binds to CDC48 and elongates mono- and diubiquitinated ERAD substrates presented by the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex to a chain length of 4 to 6 ubiquitin moieties. Delivers these polyubiquitinated substrates to RAD23 and DSK2, which target them to the proteasome. Has E3 ubiquitin-protein ligase activity, accepting ubiquitin from its cognate E2 ubiquitin-conjugating enzyme UBC4. Enhances ubiquitination at 'Lys-48', but not at 'Lys-29' of the Ub moiety. Promotes ubiquitin chain elongation at 'Lys-48' on the DOA10 substrate PEX29. Also involved in the proteolytic processing of the ER-bound transcription factor SPT23.

Belongs to the ubiquitin conjugation factor E4 family.

109.916 kDa

MTAIEDILQITTDPSDTRGYSLLKSEEVPQGSTLGVDFIDTLLLYQLTENEKLDKPFEYLNDCFRRNQQQKRITKNKPNAESLHSTFQEIDRLVIGYGVVALQIENFCMNGAFINYITGIVSNVNSYTDFLSQIIQRAILEGTALDLLNAVFPTLLEYCNKHVSHFDLNESVIYNNVLTIFELFVTFKPIAEIFTKIDGFFADYSCKPQDFERKTILGPILSLSPIEAAVAIRNYGDNLLRSKQQTAMIHESLQAEHKVVIDRLFFIVDKLVRGSLNSRTDMISYFAHIANKNHLRRADHPPFKELSSNGFMSNITLLLVRFSQPFLDISYKKIDKIDANYFNNPSLFIDLSGETRLNSDFKEADAFYDKNRKTADSKPNFISDCFFLTLTYLHYGLGGTLSFEEKMGSEIKALKEEIEKVKKIAANHDVFARFITAQLSKMEKALKTTESLRFALQGFFAHRSLQLEVFDFICGASTFLIRVVDPEHEFPFKQIKLPLIPDQIGVENVDNADFLRAHAPVPFKYYPEFVVEGPVNYSLYISKYQTSPIFRNPRLGSFVEFTTMVLRCPELVSNPHLKGKLVQLLSVGAMPLTDNSPGFMMDIFEHDELVNKNLLYALLDFYVIVEKTGSSSQFYDKFNSRYSISIILEELYYKIPSYKNQLIWQSQNNADFFVRFVARMLNDLTFLLDEGLSNLAEVHNIQNELDNRARGAPPTREEEDKELQTRLASASRQAKSSCGLADKSMKLFEIYSKDIPAAFVTPEIVYRLASMLNYNLESLVGPKCGELKVKDPQSYSFNPKDLLKALTTVYINLSEQSEFISAVAKDERSFNRNLFVRAVDILGRKTGLASPEFIEKLLNFANKAEEQRKADEEEDLEYGDVPDEFLDPLMYTIMKDPVILPASKMNIDRSTIKAHLLSDSTDPFNRMPLKLEDVTPNEELRQKILCFKKQKKEEAKHKASE