Protein
E3 ubiquitin-protein ligase ubr-1
Organism
Caenorhabditis elegans
Function
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway (By similarity). Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). In complex with ced-3, required for the ced-3-mediated cleavage and subsequent degradation of the heterochronic protein lin-28 to regulate seam cell fate patterning during larval development (PubMed:28602583). Negatively regulates glutamate metabolism through the aspartate aminotransferase got-1.2 (PubMed:29649217). Modulation of glutamate levels most likely controls locomotory behavior, in particular backwards locomotion or reversals (PubMed:29649217).
Similarity
Belongs to the UBR1 family.
Sequence
MIVDLIQSARQGEWAQVRQLLLKHWLVQVPEVFEVNSDLPWDNTAANERILGSQGEILLAPLVSAFVLDVRNTKSTLEAMNGIAGVDPARRGQICGHVFKNGELTYTCLDCATDGTCVMCLQCFEVSIHKSHKYKMHSSSGSGYCDCGDADAWTEGYACANHEKKDDEEAAVLAPELKKRCEQLVEIILQFSLSMITHKDDLKLPEIFEKMKPEVTNEAQQYLTVLYNDETHTYESVIKVLELYIHCTKDQAMLVATIVDREGRSAVKLGSKADCTKAKDDVQRKTARDPTSIRRSSNHNLPLSVKVMDTTLFALQNFSISLLTWLNTQMDVFPPLREIVGEILLSSKFALKKNYTRKMKSEDQRLVAGIIRNVMVLPDDEEEELFALDGRMDVDEMDDDDIGGEALQMEIDADDEEEITAALAGVSEHQESPGPSRDSSTFTMLENILLQDTQMWKAGRSILHQMLMRTVFMIYDQKVRFAKAFMLHYNEIYEDFIKDDHEMDVSVVGLSVQFMTVPSLARKLVAEDQAFSVISKAIRDQTDKFVKYYNDGKIARFDFTSRSFPPELRRSLHITRDMAYILNAVPSESDWNRELIDGFVQGFADFLLFLQHLQGMDEVKRQAVEHQVWESEWETAFNILLRLKDAISMIIGWAETNEEVHNRLMIMCLELMNRMPPVYTKSEEDTYELTVTINGESCRISHFDVLKSSTSVHQPVVRIIAGLFSASNYTGFLLNRSNNSHTSLNQERIKELINCKDETNLYELSLRVLVLCAQSNATLWRRNGFSLINQIHNYFSPLCRNEMFDRDVLMMQVGAALTPSTKFIFHLLHRFRLFKWATSEFEQDKANEKPAKPESEDLSKTLVMIAEEFIQCLILILCERYTYGVGKTRPMDQMKREVIHILCTGSHTFSHIQQKMSHDINSKRLSLHEAVNLVADFRKPLATTAGQFHCKESSLPTYSPFFMHYSKSDQSAAEQSQARVRAKMEKSVRACAPPILPDFQTFFERIPEILTTNVLIHVVRLIIDRTARRSRFSSDRLFHKTLYLIGIALNEEEKNPSFGFTQRAEESIGLLSLLEGLVGKPESSICPILLEVTVEKYRKLIKARAGVPEAAPAPENKPAQSEEEIKAKRAARAAEMRQKAMAKMSNMQSKFMKKIEDEEKKDESQTPSEKSETVVKKDDYDNKHFFDEDVVKQVGHDFPVCIGANKWHAELVKPRTLTCILCQEDEIIAPQQGKPMVCAAFIQQSQLFTHKNKNGELMTASSGTISTRDLLTAPATLQYGVDVSTCSHSMHYECYRSLAEANRSRESLRARQVGQHSHKMVDTENGEYQCPLCKRLSNAAIPVLPAYQLTNQNGFSTVSGAGKENFDTWVARVKRNLEMPLSSESVSKKGHSRKRSHSERSLLDLEKLSKDPDTANTSAGVLQFGAMEMSSATHMPASAESQMLMTTSPSQDDVEFYNELAAMFVDQDVNNTTSPAATPETIPAIGSSSRIPESQESGKKPLSSQIQHVLYSLIRPFPALINSNRICSSSFEGFEEPIKDLGKNMMKFRKRGNELKTNFIEKHLKGYVISTVTWQSTAHVARAISSYLHYDKKPLFGALNTRQRDCLSAMARLCASLSHNMQFLLHAVSDMLRVFLCEPPRPKLAQTPGSPLLSAPSTSSFTPAPAQIPHSGTNFAFLVQLFNPAGPRKNVNLNILQIDILSLAISLMMTIGWTWNNGTQSMNSSSTHQKARLLTPDGSVDEAYVLRLSLLAHYFQIIATHSEADGNDVNMEEEQESQMEVDPVAAQTIRKLYALCHPFDGPLRRVDILWRKMEEGAQSLLRPIALLYHFITLVDPPEALKDPSINSSEPLFRYLGLPHKIEEQISGSMLEKLFTMWSSSIPSDQALRQDLVVQPVRPNLLVELPEKYSQLINQVATFKCPTIPIEESTSNVPTLCLVCGTILCSQAYCCQKIINKQSYGACRYHMSQCSGSVGMFLRVRDCSLVLMTTRKRGCFRPAPYVDEFGEVDQGFRRGNPLHLNPELYQKLKSLWLQQGITEEVVNYNEIDFRNVQYDWGHF