Protein
Probable tRNA-splicing endonuclease subunit tsp-5
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Function
Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. May be required to embody the molecular ruler of the complex (By similarity).
Similarity
Belongs to the SEN54 family.
Sequence
MPLDDDLEDNPSLVPPPSTTTTSSNATGDAATMMDEVEEAEDAPTADFRLFASTYSKTKNISAQTIRKGEKDFESHGTRAQASALDASRDAMRDVLSYTRVHNTKSASGWCRGWYFPDWWKDWPEDWEQQKLTKRGKEGAGEDDEEEKDRKLPPLHVRDRVVLLEHTNVASQSLGRAVTGLPKDRPARGREWLLPEEALYLVERGSLDLWWPTKGIEEVFPADGSVPAAAAATTSAKGEGEQRTEEDEEDDDEYKYGLPLSLQAAYALLIGEDGERGKVSLQKFQVFSHLKRAGYNVIRAPTNPLPVQEDTQLTTTTQPASKPISVTEWLISCLPQSKSSPTDPPPYGPLVPPGFYRSYNTIYNYLSLRPSSTSSSASPTADNQPQKPQSPESDESDSGSDSPYKIHYHVYKASTKFTRTRPPPPDFYISVISAKDTSIPTLSEISSLLASAPADLPKAEWLAGGPARLYARLKHGYRNVLLAVNDHGVINYMRFAEGGFAKEELFRNYDMRVSGGPRRGGGGGGKKSGNNNGRGSRGRGGGRGGGRGGRGGRGRGN