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tsaB

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Length
236 amino acids
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD (By similarity).
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
26.084 kDa
Sequence
MQNLTLLALDTSTEACSVALLYRGEKTHINELAQRTHTKRILPMIDEILANSGLGLNQVDALAFGRGPGSFTGVRVGAGIAQGLAFGADLPVIPISNLTAMAQAAFELHQAENVVAAIDARMNEVYFSQVVREKVRSDFGEFFQWREIISEQVCSPEQAINQLQNDNAFRVGTGWAAYSQFTEKNLTGSDIALPNALYMLELAQVEYLQKHTISALEIEPIYLRNEVTWKKLPGRE

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Length
233 amino acids
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD (By similarity).
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
25.151 kDa
Sequence
MSAKILAIDTATENCSVALLVNDQVISRSEVAPRDHTKKVLPMVDEVLKEAGLTLQDLDALAFGRGPGSFTGVRIGIGIAQGLAFGAELPMIGVSTLAAMAQASYRLHGATDVAVAIDARMSEVYWARYSRQENGEWIGVDEECVIPPARLAEEAQADSKTWTTAGTGWSAYQEELAGLPFNTADSEVLYPDSQDIVILAKQELEKGNTVPVEESSPVYLRDNVTWKKLPGRE

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Escherichia coli (strain K12)
Length
231 amino acids
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. In fact, can act as a protease that specifically degrades TsaD in vitro; therefore TsaB may post-translationally regulate cellular pools of TsaD via proteolytic degradation. Does not show sialoglycoprotease activity against glycophorin A.
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
25.181 kDa
Sequence
MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQDILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGAWRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEWVTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYLRNNVAWKKLPGKE

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Length
231 amino acids
Function
Has been identified as the first Gram-negative bacterial RPF (Resuscitation-Promoting Factor) capable of reviving VNC (viable but non-culturable) cells when added externally, but the million-fold lower activity as compared to Gram-positive bacterial RPFs may indicate a lack of physiological relevance.
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
24.742 kDa
Sequence
MRILAIDTATEACSVALWNNGTINAHFELCPREHTQRILPMVQEILAASGASLNEIDALAFGRGPGSFTGVRIGIGIAQGLALGANLPMIGVSTLATMAQGAWRKTGATRVLAAIDARMGEVYWAEYQRDAQGVWQGEETEAVLKPERVGERLKQLSGEWATVGTGWSAWPDLAKECGLTLHDGEVSLPAAEDMLPIASQKLAAGETVAVEHAEPVYLRNEVAWKKLPGKE

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Bacillus subtilis (strain 168)
Length
229 amino acids
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD.
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
25.243 kDa
Sequence
MTILAIDTSNYTLGIALLREDTVIAEYITYLKKNHSVRAMPAVHSLLNDCDMAPQDLSKIVVAKGPGSYTGVRIGVTLAKTLAWSLDIPISAVSSLETLAANGRHFDGLISPIFDARRGQVYTGLYQYKNGLLEQVVPDQNVMLADWLEMLKEKDRPVLFLGHDTSLHKQMIEDVLGTKGFIGTAAQHNPRPSELAFLGKEKEAADVHGLVPDYLRLAEAEAKWIESQK

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)
Length
221 amino acids
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD (By similarity).
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
25.662 kDa
Sequence
MSNIILSIESSLDCCSVAIYKNEYIHSLSEKCKKKHTTHILPMIKEILSQTKTEFKELNYVSFSKGPGNFTSIRIAASIAQSLSISLKIPIISVSTLAIMAEKTFRKYKQKDVIVAIHAKKKQVYWAKYTRNKNSIWIGEYTESLLEKKIIQEKIENLKKRWTLVSDQSELIEFQNILNVKKNYVFLPNAKDIIPFVLLKIKNKNKSFSIENNINYLYNQF

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Length
221 amino acids
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD (By similarity).
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
25.185 kDa
Sequence
MLKTILAFDTSMSVCSISLLHKNRKYNIQKECRNNHTLYLLPMIHEILKKNHVSLNEINIIATSKGPGSFSGVRIALAVAQGISLGLNLPHVISLSTILIMAEQVWNNHKISKVLAIITVNKTSVYWIQYSRNFHGLWIKKSKAIILNLSKALEKILSLKKQWALVGNGWDKFPKNILKKIFITNITFPNSKYIISLISANSIYQKKEVLHKIIPIYLNER

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Length
210 amino acids
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD (By similarity).
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
24.122 kDa
Sequence
MSDIILAIDTSIDHCSVAVYKKKVIYSLSENCKKEHTIKILPMIKKVLMNAKITLKDLNYVAFSKGPGKFTGIRISIGIAQSLSLSLKIPIFGISTLSILAQKAWRKYKKKQILVAVNAKIGQVYWGEYLRNNTLLWTGEKTESLIKINKIEDKMKKLKKKWILVGDGWKKIKRENSLKFEQKKILYPNAKDIIPFLFSENSKPRILSFF

Gene
tsaB
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Length
206 amino acids
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD (By similarity).
Similarity
Belongs to the KAE1 / TsaD family. TsaB subfamily.
Mass
22.987 kDa
Sequence
MNVLALDTSQRIRIGLRKGEDLFEISYTGEKKHAEILPVVVKKLLDELDLKVKDLDVVGVGIGPGGLTGLRVGIATVVGLVSPYDIPVAPLNSFEMTAKSCPADGVVLVARRARKGYHYCAVYLKDKGLNPLKEPSVVSDEELEEITKEFSPKIVLKDDLLISPAVLVEESERLFREKKTIHYYEIEPLYLQKSIAELNWEKKKRG