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DNA topoisomerase 1

1212 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

130.689 kDa

MKLVVVESPAKAKTIEKYLGSGYQVLASYGHIRDLPSKDGSVNPDNGFSMVWQNYPDKAHRLKAIEDAVKESDSLILATDPDREGEAISWHILELLKTKKLLPDDVERVTFNAITKAAVTDAMAHPRQLDNDLINAYLARRALDYLVGFTLSPILWRKLPGAKSAGRVQSVALRLVVDREQEIEGFKPQEYWSVEADMEADSIGFTSRLIQWRGQKLEKLSIADKTQAEAAQSDVESGHFTVSNVETKPVSRNPQPPFTTSTLQQEAARKLGFAASHTMRLAQSLYEEGLITYMRTDGVQMDESAIAAARKTITQRYDGGYVPDKPRQYQVKAKNAQEAHEAIRPTDFSRDKAASGDHARLYELIWKRALASQMASAKLERTTVDLTDGTGQNVLRVTGQVVLFSGFLTLYEESADDNANDRDGKEGRARLPLLRAGDAPLKKEVRADQHFTQPPPRYSEASLVKKMEDLGIGRPSTYASVIQVLKDRAYVTLERNRFIPSEAGRLLTAFLERFFERYVSYDFTAGLEDSLDEISGGRADWQKVLDAFWHDFKPKTAEVMEQQPSAITAELDKFLEPWLFPDKGDGHDPRECPSCHTGRLALKGGRFGAFIACSNYPECKYTRGFGQGEDGVDDNEPVLLGYMPENSDDDGYMAFSDQGDILPANTASASETGVTDGGIAANAAFSGKGNSASHTDRDDLPFDPDEPASSTGNVASSQSRMTGDETASSGNSRDSSAHGVSTTAGIDAESQAGISNQALAGKNNAGRTAVSDNKGNNSSSTIAAARKGGSTDDNATVSDPDGDIGSGASSSGQDADNRLLSHRNGDIDSRAIPADHKDSSSDQNASHALSPDRNSDDASVSNSDKKIDSKAVSTGHDVGNAITSDNSPSDNVAHLASTPSSATSSVKVALETENNDTAASKADEQAKEEEESRKARAVTRRTGRFGPYIQLGEGKNAKRVSVPRDVNPREVDFSLASRLLALPREIGLHPESGKMIIAGTGRYGPYLNCDGKYARLSSTEELLDIDIDRAVVKLAEAAQNKGRTGATLSREPLKVFGDSPVTEKPVQLMNGRYGPYVTDGETNASLPKDTTPESLTFEEALALLEARAKMPKKKKTKKAAAKKPAAKKTTTKKAAPKKATTKTATPKSATTDNATSEDGDATPAKSPAKKAVAKKTTAKKPASKSATKKAPSSKTTAAKKTSKATPKDEVAE

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DNA topoisomerase 1

1060 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Belongs to the type IA topoisomerase family.

121.182 kDa

MILVIAEKPNVARKIAMALAEVKPIKKTLFGVPYYELIRDGKRLIVASAVGHLYGLAPKNDFFGYPIFDVEWVPVYIAEKGKEYAKDYIKLLSVLSKRVREFIVACDYDTEGEVIGYTALKYACGVDPGVAKRMKFSALTKRDLIKAWYNLEPTINFGMANAGIARHILDWYWGVNLSRALTHAIKRASGKWVVLSTGRVQGPTLKFLVDREREIQSFVPQPYWVIKLIFEKNGKKYTANYEKDKIWDENEAKRIVLEVKKAPARVTNIEVKQQNRNPPVPFDLGTLQREAYSAFGFSPKKTLDIAQSLYEKGFCLHPDTLILTSQGVRKIKELSREGEVFALDFNLKLSKAKYRLLERDADEQMYKVTLLDGTELYLTADHPVLVYREGNLAFVPADKLRETDHVVLVLNKSARDNYGFLDLLLEITDSQEDYAILENGETLSLHSLKMLVERGEIKDIAVVGFSHNNFGKVMLRDELWYLIGYLAGKGGEIKGNGVVISSRTKEIVGLTKSLNIDLIETEEGIVLSNKSFVRLLHLIHYTPRVPEVYGIINNTEWLKAFLAGYYDATLLEGLTLEALYKIKVYLQLLGIRAKIEDNKLKVHLEDLQRFRELLGKFSRRKLYVETSQVPVFTDFDERSYDFPRILGGDIYIIGIKSIEKFHYKGKVYDLVVENYHNFIANGIAVHNCSYPRTESQKLPKNLNYKFIIQNLARIPEYRPYAHLLLGMPELKPVEGKKEDPAHPAIYPTGEIPRPGDLTKDEALLYDMIVRRFLAVFMEPAVRETVKVTISAGKHKFFISGGRTVKEGWLRVYGKYVKFDEVTLPTFFIGERIKVLQVKREKKKTKPPARYSPAAVIKKMEDLGLGTKATRAQILETLYQRGYIEGKKSIKVTPLGMKVIETLEKYVPEIISVELTREFEKKMELIMEGKLTKEEVIEEAKIRLTKILEEFKKKELEIGLELAKIVVGEEKPPLIVGKCPKCGGDLIVKYNEKTGKRFVGCSNWPKCNVTYPILQRGEIIPTNKTCCNGAPVVIIREKDGREWEICLDMNCKEYNTIKKKR

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DNA topoisomerase 1

952 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

103.585 kDa

MSPTSETAKGGRRLVIVESPAKAKTIKGYLGPGYVVEASVGHIRDLPSGAAEVPEKYTGEVRRLGVDVEHDFQPIYVVNADKKSQVKKLKDLLKESDELFLATDEDREGEAIAWHLQEVLKPKIPVKRMVFHEITKDAIRAAVANPRELNQKLVDAQETRRILDRLYGYEVSPVLWKKVMPRLSAGRVQSVATRLVVERERERIAFRSAEYWDLTGTFATGRAGDASDPSSLVARLQTVDGRRVAQGRDFDSLGQLKSANTLHLDEANARALAAALENTRFAVRSVESKPYRRSPYAPFRTTTLQQEASRKLGFGAKSTMQVAQKLYENGYITYMRTDSTTLSDTAVSAARAQVTQLYGADYLPPQPRTYAGKVKNAQEAHEAIRPSGDRFRTPAETGLTGDQFKLYELIWKRTVASQMKDATGNSVTVKIGGAASDGRDVEFSASGKTITFHGFLKAYVEGADDPNAELDDRERRLPQVAEGDALTAEEITVDGHATKPPARYTEASLVKELEEREIGRPSTYASIIGTILDRGYVFKKGTALVPSFLSFAVVNLLEKHFGRLVDYDFTARMEDDLDRIARGEAQSVPWLRRFYFGEGDGTGGGGAADAGNGDGDHLGGLKELVTDLGAIDAREVSSFPVGNDIKLRVGRYGPYVERGEKDAENHQRADVPEDLAPDELSVELAEELLAKPSGDFELGTDPATGHAIVAKDGRYGPYVTEVLPEGTPKTGKNAVKPRTASLFKSMSLDTVTLDDALKLMSLPRVVGADAEGVEITAQNGRYGPYLKKGTDSRSLQTEDQLFEITLEEALAIYAQPKQRGRAAAKPPLKELGTDPVSEKPVVVKDGRFGPYVTDGETNATLRSDDSVEEITPERGYELLAEKRAKGPAKKTAKKAVKKTAAKKAPAKKAAATKKTAAAKTTAAKKTAAKSTAKKTTAKTAAKKATASKTSED

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DNA topoisomerase 1

947 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

104.056 kDa

MKGPRGSRSGRNGSPRRLVIVESPTKARKLSGYLGSAYIVESSRGHIRDLPRTAADVPAKFKSEPWARLGVNVDADFEPLYIITPEKKSTVTELKGLLQGVDELYLATDGDREGEAIAWHLMQTLKPRVPVKRMVFHEITEHAILAAAAHPRELDIDLVDAQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVQRERDRMAFCSAVYWDIVAKLDASVSDPTAQPLTFVARLTAVDGLRVATGRDFGAMGTLRGDCEVSKIIVLDDLTATTLAAGLRGAQLTVASAEEKLYTRRPYPPFMTSTLQQEAGRKLRFSAERTMSIAQRLYENGYITYMRTDSTTLSESAINAARTQARQLYGEEYVSDSPRQYTRKVKNAQEAHEAIRPAGETFATPDAVCNELDGDEFRIYELVWRRTVASQMADARGTTLSLRIEGRAGEQHVVFSASGRTLTFPGFLKAYVETVDELAGGEADDAERRLPHLTSGQLLDVIDLTPDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTVQDRGYVQKKGSALVPSWVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIANGNEQRTNWLNNFYFGGNHGVSDSIARSGGLKKLVGVNIEGIDAREVNSIKLFDDEHGRPVYVRVGKTGPYLERLLAGDDGELTPQRANLNGTLTPDELTLEVAEELFATPHEGRVLGVDPETGHEIVAKDGRYGPYVTEVLPKHDDDYGAADQGTKKTKKGRRASASQGPKPRTGSLLRSMDLQTITLEDALKLLLLPRVVGVDPASGEEITAQNGRYGPYLKRGKDSRPLTTEDQMFIITLDEALKIYAEPKRAGRQSTSPPPLRELGTDPTSGKPMLIKDGRFGPYVTDGETNASLRKGDDVVSITDERAAELLADRRARGPVKRPAKKARKVPAKKAARLAPARGISQSPR

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DNA topoisomerase 1

936 amino acids

Relaxes negatively (but not positively) supercoiled DNA, concatanates and knots circular ssDNA at 52 but not 37 degrees Celsius (PubMed:9593741). Preferentially nicks supercoiled DNA at C(G/T)CTT, cutting between the TT residues, binds ss and dsDNA with the recognition site (PubMed:10734203).

Belongs to the type IA topoisomerase family.

102.499 kDa

MAGGDRGSGGTGNVRRLVIVESPTKARKIAGYLGSNYVVESSRGHIRDLPRNAADVPAKFKSEPWARLGVNVDQNFEPLYIVSPEKKSTVTELKGLLKDVDELYLATDGDREGEAIAWHLLETLKPRVPVKRMVFHEITEPAIRNAAENPRDLDIALVDAQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVQRERERMAFHSASYWDVTAELDASVSDPSASPPKFTAKLNTVDGRRVATGRDFDSLGQLKRPDEVLVLDEASAGALASGLRGAQLAVTSVEQKPYTRRPYAPFMTSTLQQEAARKLRFSSERTMSIAQRLYENGYITYMRTDSTTLSESAINAARTQARQLYGEEYVHPSPRQYTRKVKNAQEAHEAIRPAGDVFQTPGQLHSALDTDEFRLYELIWQRTVASQMADARGTTLSLRIGGSASSGEQVVFNASGRTITFPGFLKAYVESIDELAGGESDDAESRLPNLTQGQRVDAADLSADGHQTSPPARYTEASLIKALEELGIGRPSTYSSIIKTIQDRGYVQKKGSALVPSWVAFAVVGLLEQHFGRLVDYDFTAAMEDELDEIANGQEQRTNWLNNFYFGGEHGVEGSIARAGGLKQLVGGNLEGIDAREVNSIKVFDDSEGRPVYVRVGRNGPYLERMVDDPDNPGEQKPQRANLKEDLTPDELTPELAEKLFATPQEGRSLGIDPETGHEIVAKDGRFGPYVTEVLPEPEDGGDDGTAGTPAKKGKKPTGPKPRTGSLFRSMDLETVTLEDALKLLSLPRVVGVDPTTNEEITAQNGRYGPYLKRGTDSRSLATEDQIFTITLDEALKIYAEPKRRGRQAASAPPLRELGNDPVSGKPMVIKDGRFGPYVTDGETNASLRKGDDVLTITDERASELLADRRARGPVKKKAPAKKAAKKAPAKKAAAKKA

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DNA topoisomerase 1

934 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

102.336 kDa

MADPKTKGRGSGGNGSGRRLVIVESPTKARKLASYLGSGYIVESSRGHIRDLPRAASDVPAKYKSQPWARLGVNVDADFEPLYIISPEKRSTVSELRGLLKDVDELYLATDGDREGEAIAWHLLETLKPRIPVKRMVFHEITEPAIRAAAEHPRDLDIDLVDAQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVARERDRMAFRSAAYWDILAKLDASVSDPDAAPPTFSARLTAVAGRRVATGRDFDSLGTLRKGDEVIVLDEGSATALAAGLDGTQLTVASAEEKPYARRPYPPFMTSTLQQEASRKLRFSAERTMSIAQRLYENGYITYMRTDSTTLSESAINAARTQARQLYGDEYVAPAPRQYTRKVKNAQEAHEAIRPAGETFATPDAVRRELDGPNIDDFRLYELIWQRTVASQMADARGMTLSLRITGMSGHQEVVFSATGRTLTFPGFLKAYVETVDELVGGEADDAERRLPHLTPGQRLDIVELTPDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTIQDRGYVHKKGSALVPSWVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIAAGNERRTNWLNNFYFGGDHGVPDSVARSGGLKKLVGINLEGIDAREVNSIKLFDDTHGRPIYVRVGKNGPYLERLVAGDTGEPTPQRANLSDSITPDELTLQVAEELFATPQQGRTLGLDPETGHEIVAREGRFGPYVTEILPEPAADAAAAAQGVKKRQKAAGPKPRTGSLLRSMDLQTVTLEDALRLLSLPRVVGVDPASGEEITAQNGRYGPYLKRGNDSRSLVTEDQIFTITLDEALKIYAEPKRRGRQSASAPPLRELGTDPASGKPMVIKDGRFGPYVTDGETNASLRKGDDVASITDERAAELLADRRARGPAKRPARKAARKVPAKKAAKRD

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DNA topoisomerase 1

934 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

102.336 kDa

MADPKTKGRGSGGNGSGRRLVIVESPTKARKLASYLGSGYIVESSRGHIRDLPRAASDVPAKYKSQPWARLGVNVDADFEPLYIISPEKRSTVSELRGLLKDVDELYLATDGDREGEAIAWHLLETLKPRIPVKRMVFHEITEPAIRAAAEHPRDLDIDLVDAQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVARERDRMAFRSAAYWDILAKLDASVSDPDAAPPTFSARLTAVAGRRVATGRDFDSLGTLRKGDEVIVLDEGSATALAAGLDGTQLTVASAEEKPYARRPYPPFMTSTLQQEASRKLRFSAERTMSIAQRLYENGYITYMRTDSTTLSESAINAARTQARQLYGDEYVAPAPRQYTRKVKNAQEAHEAIRPAGETFATPDAVRRELDGPNIDDFRLYELIWQRTVASQMADARGMTLSLRITGMSGHQEVVFSATGRTLTFPGFLKAYVETVDELVGGEADDAERRLPHLTPGQRLDIVELTPDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTIQDRGYVHKKGSALVPSWVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIAAGNERRTNWLNNFYFGGDHGVPDSVARSGGLKKLVGINLEGIDAREVNSIKLFDDTHGRPIYVRVGKNGPYLERLVAGDTGEPTPQRANLSDSITPDELTLQVAEELFATPQQGRTLGLDPETGHEIVAREGRFGPYVTEILPEPAADAAAAAQGVKKRQKAAGPKPRTGSLLRSMDLQTVTLEDALRLLSLPRVVGVDPASGEEITAQNGRYGPYLKRGNDSRSLVTEDQIFTITLDEALKIYAEPKRRGRQSASAPPLRELGTDPASGKPMVIKDGRFGPYVTDGETNASLRKGDDVASITDERAAELLADRRARGPAKRPARKAARKVPAKKAAKRD

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DNA topoisomerase 1

934 amino acids

The C-terminus (residues 622-934) inhibits RNA cleavage by MazF4.

Belongs to the type IA topoisomerase family.

102.336 kDa

MADPKTKGRGSGGNGSGRRLVIVESPTKARKLASYLGSGYIVESSRGHIRDLPRAASDVPAKYKSQPWARLGVNVDADFEPLYIISPEKRSTVSELRGLLKDVDELYLATDGDREGEAIAWHLLETLKPRIPVKRMVFHEITEPAIRAAAEHPRDLDIDLVDAQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVARERDRMAFRSAAYWDILAKLDASVSDPDAAPPTFSARLTAVAGRRVATGRDFDSLGTLRKGDEVIVLDEGSATALAAGLDGTQLTVASAEEKPYARRPYPPFMTSTLQQEASRKLRFSAERTMSIAQRLYENGYITYMRTDSTTLSESAINAARTQARQLYGDEYVAPAPRQYTRKVKNAQEAHEAIRPAGETFATPDAVRRELDGPNIDDFRLYELIWQRTVASQMADARGMTLSLRITGMSGHQEVVFSATGRTLTFPGFLKAYVETVDELVGGEADDAERRLPHLTPGQRLDIVELTPDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTIQDRGYVHKKGSALVPSWVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIAAGNERRTNWLNNFYFGGDHGVPDSVARSGGLKKLVGINLEGIDAREVNSIKLFDDTHGRPIYVRVGKNGPYLERLVAGDTGEPTPQRANLSDSITPDELTLQVAEELFATPQQGRTLGLDPETGHEIVAREGRFGPYVTEILPEPAADAAAAAQGVKKRQKAAGPKPRTGSLLRSMDLQTVTLEDALRLLSLPRVVGVDPASGEEITAQNGRYGPYLKRGNDSRSLVTEDQIFTITLDEALKIYAEPKRRGRQSASAPPLRELGTDPASGKPMVIKDGRFGPYVTDGETNASLRKGDDVASITDERAAELLADRRARGPAKRPARKAARKVPAKKAAKRD

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DNA topoisomerase 1

898 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

99.34 kDa

MSKLVIVESPTKARTIRNYLPQDYRVEASMGHVRDLPASAEEVPAAYKDKSWANLGVNVEDHFSPLYVIPKSKKKVVKELQTALKNADEVILATDEDREGESISWHLLQLLQPKVPIKRMVFHEITQEAIRSALENCRDIDENLVHAQETRRILDRLVGYTLSPLLWKKIAWGLSAGRVQSVAVRLIVQRERARRAFKTAGYWDLKAELEQNKNPFQAKLMTLGGTKLANGSDFDPNTGALLPDKQVVVLDEAQAIALKERLTGKPWAVVNTEEKPGVRKPSPPFTTSTLQQEANRKLGISARDTMRVAQKLYEEGYITYMRTDSVHLSDQAVTAARNCVQQMYGKEYLSPQPKQYTTKSKGAQEAHEAIRPAGTEFRIPNQTGLKDRELALYELIWKRTVACQMADARITQLSVLLKVEDAEFRAAGKRIDFPGYFRAYVEGSDDPDAALENQEVILPPLKVGDRPNCREIDTVGHETQPPARYTEASLVKTLESEGVGRPSTYASIIGTIIDRGYVQMRSKALTPTFTAFAVVSLLESHFPDLVDTGFTSRMEQKLDEIAIGKTQWLPYLQGFFLGESGLENQVKVRQDQIDPAIAKAIELENLAAKVKIGKFGPYIEIPQGEEIITASIPQDLTPADLNPEQVAVLLKQKTEGPDKVGIHPETGEPIFILIGAYGPYVQLGEATEENKKPKRTSLPKGVKPEDVTLEMAVGLLALPRNLGEHPESGKNIKASLGRFGPYVVHDQGKDGKDYRSLKGDDDVLTITLERALELLAEPKRGRGSRTPLKQLGLHPEDQEPVNLFKGPYGVYIKHGKVNASLPEGETEETITLEKALPLLAEKAGSGKKTSRKKATTTAKGTKKTTSKKASATGTAKKTTTKRTTRKKAASPDQSSEAG

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DNA topoisomerase 1

883 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

98.296 kDa

MPKLVIVESPTKARTIRNYLPKDYRVEASMGHVRDLPQSASDIPTELKGEKWSNLGVDVENNFAPLYIVPKDKKKIVKTLKDALKDADELILATDEDREGESISWHLLQLLQPRVPTKRMVFHEITQEAIQAALKNCRDVDQRLVHAQETRRILDRLVGYTLSPLLWKKIAWGLSAGRVQSVAVRLLVQRERARRAFRQGSYWDLKAQLTVEAGQFEAKLWTLAGQRLATGNDFDESTGQIIAGRQVCLLDQQEAEALRDRLQTQPWQVKSLEEKPTTRKPAPPFTTSTLQQESNRKLRLSARETMRVAQSLYERGFITYMRTDSVHLSQQAIEAARSCVEQMYGKNYLSPQPRQFTTKSKNAQEAHEAIRPAGNTFRLPQETGLSGAEFALYDLIWKRTIASQMAEARQTMLSVLLEVDNAEFRASGKRIDFPGFFRAYVEGSDDPDAALEDREILLPALKVGDRPTCQELAAIGHETQPPARYTEASLVKMLENEGIGRPSTYASIIGTIVDRGYAQLVSNTLTPTFTAFAVTALLEQHFPDLVDTSFSARMEQSLDDISNGEVDWLPYLSQFYRGDRGLEEQVKLRESEIDPAAARTVALEGLPAKVRIGRFGAYLEAEADGEPIKANLPKELTPADLDVQRVETLLRQKTEGPDQLGTHPETDEPIYLLTGAYGPYVQLGAATEEKPKPKRASLPKGMSLETISLEQAVGLLSLPRTLGEHPETGRRIQAGLGRFGPYVVCDLGGGEKDYRSLKADDDVLTIDLDRALELLAQPKKSRGRGKEPIREVGLHPDDQAPIQIFEGPYGLYLKHGKVNASLPEDEKPETISLETAVAALAAKAGQAKAKGGRRSTGTPKSGETKARTTKTTKKTTTRRTTSR

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DNA topoisomerase 1

876 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

97.91 kDa

MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPTAGQTATPTGKAAAASTKKASTTDKEQQKREKERAALIKKMGVDPYHGWQANYQILPGKEKVVAELQKLAKDADSVYLATDLDREGEAIAWHLREIIGGDEKRYKRVVFNEITKNAIQQAFKQPGELNMDGVNAQQARRFMDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFVPEEFWDINADTLTADKQDFRLLVAQKDGLAFKPVNETEALAAVAALNKAQYEVCKREDKPTTSKPSAPFITSTLQQAASTRLGYGVKKTMMLAQRLYEAGYITYMRTDSTNLSAEAVENLRGYITKHYGEAYLPSQPNVYGSKQNAQEAHEAIRPSDVTVTADDLEGMEADAHKLYALIWNQFVACQMTPAQYDSTTVSVKAAEYTLKAKGRILKFDGWTRVQRPLGKNEDQILPPVKVGDSLKLVSLDPKQHFTKPPARYTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVDQRRFFAEKMGEIVTDRLDENFDDLMNYDFTARMEEKLDQIAEGEVNWTAVLDEFFADFSRDLETAEQDESLGGMKPNHIVMTNILCPTCSRPMGIRTASTGVFLGCSGYGLPPKERCKTTINLGDEEGVINVLEEDVETAALRAKKRCPICETAMDAYLIDDKRKLHVCGNNPNCEGFIVEEGEFKVKGYEGPTVECDKCGSDMVLKNGRFGKYMGCTNDACKNTRKILKNGEVAPPKEEPVHFPELPCENSDAYFVLRDGASGLFFAASNFPKSRETRAPLVEELKRFAERLPEKYQYLTSAPAHDPDGLPAVVRFSRKEKEHYVRTETEGKPSGWMAVYQEGKWLVTDKRKNAK

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DNA topoisomerase 1

868 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

97.283 kDa

MGKSLVIVESPAKAKTINKYLGSQYVVKSSIGHIRDLPTSGSSSSKEPAAKGRKSASEAPALSPKEKARRQLVSRMGVDPEHGWKAKYEILPGKEKVIDELRRLAKDADTVYLATDLDREGEAIAWHLREAIGGDESRYKRVVFNEITKKAIQEAFSQPGELDINRVNAQQARRFLDRVVGYMVSPLLWQKIARGLSAGRVQSVAVKLVVEREREIRAFVPEEYWEVHADLGTAKGANVRFEVTREKGEAFKPLNEAQAMAALEKLKASAYSVAKREDRPTSSRPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSADAIGMVRGFIEDEFGQKYLPGKANVYSSKEGAQEAHEAIRPSDVNLKPTQLSGMERDAERLYDLIWRQFVACQMTPAEYLSTSVSVTAGDFELRAKGRILKFDGYTRVLPQQSKPGEDDVLPEMKEGENLKLIKLDPSQHFTKPPARYSEASLVKELEKRGIGRPSTYAAIISTIQERGYVTTHNRRFYAEKMGDIVTDRLNESFANLMDYGFTAGMEEHLDDVAQGERDWKHLLDEFYGDFKKKLEVAEVSEKGMRANQPTLTNIPCRECGRPMMIRTASTGVFLGCSGYSLPPKERCKATVNLIPGDEIAADDEGESESRVLRGKHRCPICSTAMDAYLLDEKHKLHICGNNPDCPGYEIEEGQYRIKGYEGPSLECDKCGSEMQLKTGRFGKFFGCTNPTCKNTRKLLKNGEAAPPKMDAIRMPELKCEKVDDIYVLRDGASGMFLAASQFPKNRETRAPLVSEIIPHKAELDPKYHYLCDAPQKDPDGRPAVIRFSRKTKEQYVQSEVDGKPTGWRAFYDGGKWKVEDKR

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DNA topoisomerase 1

868 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

98.144 kDa

MSKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEEKAKIKAEKERNALVKRMGIDPYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKPEQLNMDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAIRLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSQDALNMARTYIENHFGAQYLPEKPNFYSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLIWCQFLACQMPPAQYDSSTLTVTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPPARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESFGELMNYDFTANMEDTLDKIASGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNSLVETDIKCPTCGRNMAIRTASTGVFLGCTGYALPPKERCKTTINLIPEAELLNVLDESSETKALMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCGADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRPVKIAELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKKQYVTSEKEGKATKWIVDFTNGKWVERKK

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DNA topoisomerase 1

868 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

98.048 kDa

MSKSLVIVESPAKAKTINKYLGSNYVVKSSVGHIRDLPTVGSSNGEKAKAISTKGLSAEEKSAIKQEKDRNALVKRMGIDPYHQWKANYQILPGKEKVVAELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGDDARFSRVVFNEITKNAIQQAFEKPEQLNLDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLVVEREREIKAFQPEEYWEIDVLTQTQAKQALRLAVTQFKGKKFEPKNATQAQSAVDFLQHADYVVSDLETKPTHSRAKAPFITSTLQQTASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSQDALKMVRGYIQSHYGESYLPSKPVFYSSKENAQEAHEAIRPSDVNTLAEQLTGMDKDAVRLYDLIWRQFVACQMPAAQYDSTTVTVRAGEYELKAKGRILRFDGWTKVLPTMSKNAEDQVLPDVQLNEKLHLEHVLPEQLFTKPPARFSEAALVKELEKRGIGRPSTYAAIISTIQERGYVRVENRRFYAEKMGEIVTDRLNQSFTALMNYDFTANMENILDQIANGEKNWKTELNQFFKDFSQQLSQAELDELEGGMKPNSLVPTDINCPTCGRKMAIRTASTGVFLGCSGYALPPKERCKTTMNLIPEAELLNVLDEASETKALMERKRCPKCGTAMDSYLIDPERKIHICGHNPNCDGYVLEQGSFKIKGYDGPIVECDKCGADMHLKLGRFGKYMGCTSCDNTRKILKSGEVAPPKEEPTHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRAPKVAELALYRDRLPEKLRYLADAPQHDPEGNDAIVRFSRKEKRQYVTSEKNGKATKWIVDYLGNQWVERKK

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DNA topoisomerase 1

865 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

97.303 kDa

MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWDAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFEQPGELNINRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEIDANTTTPSGEALPLQVTHQNDKPFRPVNREQTLAAVSLLEKARYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYIGDNFGKKYLPDNPNQYASKENSQEAHEAIRPSDVAVMAESLKDMEADAQKLYQLIWRQFVACQMTPAQYDSTTLTVGAGEFRLKARGRILRFDGWTKVMPALRKGDEDRTLPAVNKGDALTLLELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMEDSLDQVANHQAEWKAVLDNFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALSPKERCKTTINLVPENEVLNVLEGDDAETNALRAKRRCQKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGIFLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTVVRFSRKTKQQYVAAEKDGKATGWSAFFVDGKWVEGKK

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DNA topoisomerase 1

865 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

97.303 kDa

MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWDAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFEQPGELNINRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEIDANTTTPSGEALPLQVTHQNDKPFRPVNREQTLAAVSLLEKARYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYIGDNFGKKYLPDNPNQYASKENSQEAHEAIRPSDVAVMAESLKDMEADAQKLYQLIWRQFVACQMTPAQYDSTTLTVGAGEFRLKARGRILRFDGWTKVMPALRKGDEDRTLPAVNKGDALTLLELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMEDSLDQVANHQAEWKAVLDNFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALSPKERCKTTINLVPENEVLNVLEGDDAETNALRAKRRCQKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGIFLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTVVRFSRKTKQQYVAAEKDGKATGWSAFFVDGKWVEGKK

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DNA topoisomerase 1

865 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

97.35 kDa

MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK

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DNA topoisomerase 1

861 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

99.74 kDa

MQKSLVIVESPAKAKTINQYLGSEYIVKSSIGHIRDLTKGKLYNKEKNKKFLNENFIEKTNENKILIKQMGIDPYQNWKFEYHILPGKEKIISELKYIANQVKHIYLATDLDREGEAIAWHLKEVIGGDSSKFSRVVFNEITQHSIQKAFKNVGHINMNRVHAQQARRFMDRIVGYMISPLLWKKIARGLSAGRVQSVAVRIIADRESIIKNFVPEEYWKLDVSLISQDKKKINMDVTHYNNKKFRPINENEVSFAVEKIQKSSCIVKNYEEKISYLKAPAPFITSTLQQSASLRLGFSVKKTMFLAQKLYEEGYITYMRTDSNYLSEYAIKKVRKYIKSNYGSNYLPKEPNIYSNEKHSQEAHEAIRPSDIKIKNIDSDHLNSSAKKLYELIWNQFLASQMKSVKYKSITVTVLADMFKLQKSERIVMFQGWNKVLIEEKNVFSQFPILQTGSQLFINKVTPSQKFTKPPPRFSEASLVRELEKKGIGRPSTYSAITSKIQDRGYVKIKKNKFYAEKMGEILTIRLKKSFSNLIDYNFTAHMEKKLDQVAENKVTWRYLLDDFFKKFSEQLEQAKKSPEEGGMELNNIVPTSLNCPICCKKMGIKTAITGVFLSCLGYNNTDNKKRCKKTINLITLNDFNKEQDNQKKISLQLIQKCDICNMYMDSYFINEKLKLHICANNPSCSGYKFEKGVFKSPIYLSKTIQCEKCYNNMKLKIGPFGKFFTCINKICKNTRKILPNGEISDPKLEPIPFPELLCKQSDAWFVLREGISGIFFAANTFPKSRETRSPFVEELVRFQHLLPEKIYYLSSAPVIDNYGNKTIVCFDKKKKTHYIASKKDGKFTGWSAVFIDQKWCVINK

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DNA topoisomerase 1

853 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

98.816 kDa

MCKSLVIVESPVKAKTISKYLGKQYIVKSSVGHVRDLINNKSKNKKNIKKSNIKHNEKTALIQQMGINPYKNWKAEYHILPGKEKIISELKNIANKVHYIYLATDLDREGEAIAWHLKEVIGGNSLKFRRVVFNEITKKSIEKAFQNIGKINMNRVYSQQARRFMDRIVGYMISPLLWKKISRGLSAGRVQSAAVRLITDREYEIKNFIEREHWKISLSLLSKENKKVTMDITHYRNKTFLLNNKKEVNSTIEKIKNLSFIITDRKDKIFKKKPPAPLITSTLQQASNIDLGFSVKKTMFLAQKLYEQGYITYIRTDSYFLSNYAIEKVRSYIENFYGSDFLPKKSNIYSNQKYSQEAHEAIRPSDVQVVNIDNCDPDAKKLYKLIWNYFIASQMKSERYKSIKTTVMADVFKLQSNTKIVLFSGWTKILKKSNNVNFKFPVLDIGTTLILDKILPHQIFTKPPPRFSESSLVRELEKKGIGRPSTYATIITKIKEKGYLKIKKNKFYAAKIGEILITRLKKNFSDLVDYDFTARMEKNLDQVSDKLINWKHLLNSFFDNFSQQLEQAKKPPEEGGMELNTTVPTEIDCSLCNKKMGIKTAVTGVFLSCLGYNSEPNEKRCKNTINLISLNDLSTTKKEEKNFHLKNRCKKCNLVMDVYLINENLKIFICINNPSCNGYNLKKGIFKKSLNCSLKKIKCEKCKNDMLFKTGRFGNFFMCINDTCKNTRKILPNGEISEPKFEPIPFPDILCEKSNTWFVLREGISGIFFAANTFPKSRETRSPFVEELARFEYLLPKKLHYLASAPQRDNKGNKTIVCFNKLNKQQYIASKKEGKFTGWAAFFIDRKWCIVNK

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DNA topoisomerase 1

848 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

97.663 kDa

MQNKKLIIVESPTKAKTIKKFLDESFLVEACIGHVVDLPNNAKEIPKEYKKYEWANISIDYNNGFNPIYIIPSNKKPIVSKLKKLVKTINEIYLATDQDREGETIAFHLKEVLKIKNYKRMIFHEITETAITESLKNTRNIDMNLVNAGEARRILDRLYGYTISPLLWKKVAYGLSAGRVQSVGLKLLIEKEKTRINFKKANYYSILLQCKHEKKNLLLEAKLEEIDGKNIAEGKDFVNETGKLKNIAKTTIITQDLMIELEKELKNGQKIELISIETKKIKIPPPKPFTTSTLQQEINKRLKIGTKQIMQHAQKLYEHGYITYMRTDSHNIAKIAKDKITKIIKNKYGKEYIEEKDRIYEKEKMAQNAHEAIRPSEIFIPNETIEIESKTAKEIYKIIWDRTIISGMKDAIKENIKLTFKYKNLIFRSSFTKIIFDGFLKHTKEQDEHLNINFDLIKKGDTFSIVKMKTSEHETKAPFRYTEASLVQKMEKEGIGRPSTYSTIISTLLEREYAFKLNNTLMPTIKGAAVINLLEKYFPVLIELNFTSNMEEKLDKIAIGKLDKIKYLSKFYNGKKGLKDTVMQLEPKIDSSEFRTVIESQKIENKNSINYTINIGKYGPYLIFKGHNYSINAKTPLENLYKKDEIEKIINEKELKPNILGVDPLTGLNVIFKNTIYGNIVQLGEDTHAPQEYTKKGKPKKLKIIKAKKASTKKIDPENITLELALKLLSLPKPIGKHPQTNEQIIAATGVFGDYIKTESGSIACSLKKDLKAYDITLDKAISLLNERANKVGIIVKTITFSKNKIGNKIYIYKKNDKFYAKIKRKKIDLPDNINLEEINEKYVFSLL

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DNA topoisomerase 1

815 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

91.803 kDa

MPKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPENGFAMRYDLIDKNEKHVEAITKAAKTADSIYLATDPDREGEAISWHISEILKERGLLKDKPMQRIVFTEITPRAIKEAIQKPRMIASDLVDAQQARRALDYLVGFNLSPVLWRKVQRGLSAGRVQSPALRMIVEREEEIEAFITREYWSIHAECAHPAQHFSAKLIKLDGQKFEQFTITDSDTAAAAQRRIQQVAQGRLHITDVTNKERKRRPAPPFITSTLQQEASRKLGFTTRKTMQIAQKLYEGIALGEEGSVGLITYMRTDSVNLSLDALSEIRDIIARDYGTNALPDKPNVYTTKSKNAQEAHEAVRPTSALRTPTQVAPYLSNEEHRLYELVWKRAVASQMIPAILNTTSVDLAAGNEHVFRATGTTVVVQGFLAVYEEGKDNKNAEDDDEGRKLPVMKTGENVPLERILTEQHFTQPPPRYTEAALVKALEEYGIGRPSTYASIIQTLLFRKYVDMEGRSFRPTDIGRAVSKFLASHFTRYVDYDFTAHLEDELDAISRGEEEWIPLMKKFWVPFKELVEDKKDSLDKTDAGSVRLLGIDPTSGKEVSGRIGRFGPMVQIGTVDDEEKPRFASLRPNQSIYSISLEEAIELFKMPRVLGEDQSQQVSVGIGRFGPFAKRGSTYVSLKSEDDPYTIDLARATFLINEKEEIARNRIIKNFENSQIQVLNGRFGPYISDGKLNGKIPKDREPASLTLEEAQQLLIDTGKPARKNFSTKKTATKNETRKQTTKKRTTDAKATKKVSDKPVKKQIKKRIAPNITE

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DNA topoisomerase 1

815 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

91.802 kDa

MPKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPENGFAMRYDLIDKNEKHVEAITKAAKTADSIYLATDPDREGEAISWHISEILKERGLLKDKPMQRIVFTEITPRAIKEAIQKPRMIASDLVDAQQARRALDYLVGFNLSPVLWRKVQRGLSAGRVQSPALRMIVEREEEIEAFITREYWSIHAECTHPAQHFSAKLIKLDGKKFEQFTITDSDTAAAAQRRIQQAAQGRLHITDVTNKERKRRPAPPFITSTLQQEASRKLGFTTRKTMQIAQKLYEGIALGEEGSVGLITYMRTDSVNLSLDALSEIRDIIARDYGTNALPDKPNVYTTKSKNAQEAHEAVRPTSALRTPTQVAPYLSNEEHRLYELVWKRTVASQMIPAILNTTSVDLAAGNEHVFRATGTTVVVQGFLAVYEEGKDNKNAEDDDEGRKLPVMKTGENVPLERILTEQHFTQPPPRYTEAALVKALEEYGIGRPSTYASIIQTLLFRKYVDMEGRSFRPTDIGRAVSKFLSSHFTQYVDYDFTAHLEDELDAISRGEEEWIPLMKKFWVPFKELVEDKKDSLDKTDAGSVRLLGIDPTSGKEVSARIGRFGPMVQIGTVDDEEKPRFASLRPNQSIYSISLEEAIELFKMPRVLGEDQSQQVSVGIGRFGPFAKRGSTYVSLKSEDDPYTIDLARATLLINEKEEIARNRIIKDFENSQIQVLNGRFGPYISDGKLNGKIPKDREPASLTLEEAQQLLINTGKPARKNFSTKKTATKNETRKQTTKKRTTDAKATKKVSDKPVKKQIKKRIAPNITQ

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DNA topoisomerase 1

800 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

91.229 kDa

MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVIPDENFSMKYDISEKAGKYVDAIIKDAKKAESVYLATDPDREGESISWHIAEVIKEKNKVKSDDFFKRVAFNEITKKAITHAIENPRKLDNNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICEREDEIERFKSEEYWDISLKMLNSNNELFTAKLTHINDQKLEKFSITNDKEAKDLTEKLKSQNFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAVDEIRKLINKDYGDKYLPSSPRIYKSKVKNAQEAHEAIRPTNINYIPNDLKEKLEKDYYKLYELIWKRTIACQMENVIMDLVNATLASENKEYLARANGSTIAFDGFYKVYRESIDDEAEEENKMLPPLKEQEHLKTKEIIPNQHFTEPPPRYSEASLVKKLEELGIGRPSTYATILSVLQDRKYVTLEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDEIAAGKLEWKSALGNFWNGFNHNIESVNKQNITEIISYVQQALDYHIFGENKDSKVCPSCKTGELSLKLGKFGAFLACSNYPECNFRKSIVSGNDNNEADGEAKDIVNENKVLGKDKEGIEIYLKKGPYGPYIQYGEQVDSKIKPKRSPLPAGLNQNDITLDMALKLLSLPLKIGNHKESGEEVLVGYGKFGPYIKYMGKFISIPKKYDFLNLSLDDAMKLIEEKLNAIAAKQPNPLNMDEVTNLVDKKIKVKKTKK

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DNA topoisomerase 1

779 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

89.083 kDa

MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDKNFAMEYDISDKASKYVDAIVKYARKAEAVYLATDPDREGESISWHVAEVIKEKNKVESDDFFKRVAFNEITKKAIIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDREDEIERFKAEEYWDISLKMQNSNNELFTAKLTHVNDQKLKKFSIINEKEAKDLTRKLKLQKFYVEKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGNKYLPINPRIYQSKVKNAQEAHEAIRPTNITYTPDNLKQKLEKDYYKLYELIWQRTIACQMENVIMDLVIANLASENKEYLAKANGSIIAFDGFYKVYRESLDDEDEEDNKMLPPLKAQEHIKTKEVIPNKHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVTLEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDEIAAGKLEWKTSLNNFWSGFNNNIESVNEQKITEIINYLQKALDYHLFGENKESKVCPSCKTGQLSLKLGKFGAFLACSNYPECTFKKSIVSGNDNNEDEGNLSTILNDNKILGTDKDGVEIYLKTGPYGPYIQLGEQCGKVKPKRTPVPTNLKQSEITLEVALKLLSLPLKIGIHKDSGEEIIIGYSKFGPYIKYMCKFISVPKKYDFLNLNLDDAIKLIENNKAKLEKKHRSMV

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DNA topoisomerase 1

776 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

88.145 kDa

MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYVDAIVKDAKKADAVYLATDPDREGESISWHVAEVIKEKNQVKSDDFFKRVAFNEITKKAIIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICEREDEIERFKSEEYWDISLKMQNSNNELFTAKLTHVNDQKLEKFSIINEKNAKDLTKKLKSHKFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADIRKLIDKSYGDKYLPTSPRIYKSKVKNAQEAHEAIRPTNITYTPDSLKEKLDKDYYKLYELIWKRTIACQMENVIMDLVVANLASENKEYLAKANGSTIAFDGFYKVYRESMDDEAEEENKMLPPLKEQEPLKTKAVIPNQHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVALEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDAIAAGKLEWKAALNNFWSGFNHNIESVNEQKITEIISYVQKALDYHLFGENKESKICPSCNTGELSLKLGKFGAFLACSNYPECTFRKSIVSGNDNNENEGEPAAMPNENKVLGTDKDGVEIYLKKGPYGPYIQLGKQEGKVKPKRSPVPASLNQNDITLDIALKLLSLPLKIGIHKDSDEEIMIGYGKFGPYIKYMGQFISVQKKYDFLNLSLDDAMKLIEENKAKLEKKQA

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DNA topoisomerase 1

776 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

88.141 kDa

MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYVDAIVKDAKKADAVYLATDPDREGEAISWHVAEVIKEKNKVKSDDFFKRVAFNEITKKAIIHAVENPRKLDANLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICEREDEIERFKSEEYWDISLKMQNSNNELFTAKLTHVNDQKLEKFSIINEKDAKDLTEKLKSQNFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGDKYLPNSPRIYKSKVKNAQEAHEAIRPTNITYTPDSLKEKLEKDYYKLYELIWKRTIACQMENVIMDLVVASLASENKEYLAKANGSTIAFDGFYKVYRESVDDEAEEENKMLPPLKEQEPLKTKEIIPNQHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVSLEKKRFMPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDEIAAGKLEWKAALNNFWSGFNHNIESVNEQKITEIISYVQKALDYHLFSENKESKACPSCKTGELSLKLGKFGAFLACSNYPECTFRKSIVSGNDNNENDGDLAATPNENKVLGTDKDGIEIYLKKGPYGPYVQLGEQEGKVKPKRSPVPASLNQNDITLEMALKLLSLPLKIGIHKDSGEEIMIGYGKFGPYIKYMGKFISIPKKYDFLNLSLDDAMKLIEDNKAKLEKKQA

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DNA topoisomerase 1

776 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

88.722 kDa

MKLVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDKNFLMEYDISDKAGKYVDAIVKEARKAEVVYLATDPDREGESISWHVAEVIKEKNKVESDDFFKRVAFNEITKKAIMNAVANPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDREDEIERFKSEEYWDISLKMQNSNNDLFTAKLTHVNDQKLKKFSIINEKEAKDLTQKLKLQKFYVEKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGNQYLPIKPRIYQSKVKNAQEAHEAIRPTNITYTPDSLKQKLEKDYYKLYELIWHRTIACQMENVIMDLVIANLASENKEYLAKANGSIIAFDGFYKVYRESLDDEDEEDNKMLPPLKEQEHIKTKEVIPNKHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVALEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDEIAAGKLEWKTALNNFWRGFNHNIESVNEQKITEIINYLQKALDYHLFGEDKESKVCPSCKTGQLSLKLGKFGAFLACSNYPECTFKKSIVSGNDNNEDEGDPSTILNDNKILGTDQDGVEIYLKKGPYGPYIQLGEQCGKVKPKRTPVPANLKQSEITLEIALKLLNLPLKIGIHKDSGEEIIIGYSKFGPYIKYMCKFISVPKKYDFLNLSLNDAMKLIQNNKAKLEKKYG

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DNA topoisomerase 1

770 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

87.667 kDa

MDGFRIIIAEKADAGRRIAYFLSGGQVKSHRAKGTSYLEFEYNGSKTYLIPLSGHIVEADFESGYSDWNKIDLSDLIDARIVKNIKNKVAYQTLQAFRGKVEEIVIATDYDREGELIGVEALDIIKEGKEEIRRAKFSALTKNEILDSFKNLIGVNYSLADAADARESIDLIWGSVLTRFFSVTTGRLGKSFLSAGRVQTPTLAIVVDREREIQSFRPERYWTISITFDKDGQFKARYPENIKDQDTAEKIYEAIKGKNGRVSSYTSKEDHIRRPAPFSTTEFLREASRIGIMPTKAMSIAENLYMRGLISYPRTDNTVYPRSINLKSVLKKLENTAYSKYVKEIETFDRILPSRGRIETTDHPPIYPVDSPKEQLKGDYGRVYDLILRHFLSTLYRDGKKTVAEAEIYVNGYTFKAAGQHTTDRGWTEIYGYDPKDVYLPELTEGEDLKAIDWNIQREETKPPPRYDMSSLLKKMEELNLGTKSTRHDIIGKLIERGFIEGNPVKPTPLGMAFIDAVRSVNSHIADPEMTAKLEEDMDRIEKNEMSKNDVVEESKKMLHEVLSHFLTKTADVKDIITKGINAGQEIGDCPFHEGKKIMVIRDRFTYTVRCEDPSCKINFRIKRNGSITLSDQKCPVCGLPMIKIIRKGQSPEIKCIDPDCSYNRENEDYGECPADHGRLVLRQSKYGKRFLGCSNYPKCTVTYPLPQMGRITKTGEVCPYCGAPILALSRNGRKWKFCPNMQCEYNKKRKPEAAVEKSVRKKGRNASKS

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DNA topoisomerase 1

761 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

87.833 kDa

MAEIMTALIICEKPSVAKKIANALGKAKKKSIDGVPYYELERDGKKIIVASAVGHLFTLVEKENKEFGFYPVFDIKWVPASVDKGKEYVNKYIKALKKLSKDADEFYIATDWDIEGELIGYHALKYCCGREKAKRMRFSSLTKKEIVRAFENPDEIDYGLVDAGESRHILDWYFGINLSRALMNAIRAVNRWKTMSVGRVQGPALAFLTERELEIKKFIPKPYWVIEALLKDNLKAIHEKEKFWNEKEAKNVYEKIKDEKSAKVVEIKKTKRKLKPLPPFDLGTLQREAYSYFKISPKETQEIAQKLYENALISYPRTSSQKLPKDRKYLEDILNIIKNHPVYGKWAERILKENLKPVEGKKEDPAHPAIHIVDIPKEELSEKEKEIYDLIARRTLAAFWDNAEREYLNVKIDIKGEKFKLSGSRTVKEGWHEIYYFPKFDEIELPPLKKNDIIKVEKITITRKETQPPKRYTVASIIKELEKRGLGTKATRAEIIDKLIKRGYVIDDGSLKVTDLGISVIETLKRFCPEIIDEKMTRDLEEKLEKIQFRKIKKDDVLDEAEKRLRKILEEFKKKEEDIGIYLIKNLDATNKKAKIVGKCPKCGGDLILIRHKKGRFVGCSNYPECDVKYSLPDKGRIKIPNKVCDACKSPILKIGDREICINPECPLKQVEVKEEDRICPKCGAKLILKKGVYGAFYGCSNYPKCKYTEPINKKEVVGKCPKCGGDLVVREGKFGKFVGCSNYPKCRYTEKLKLNEKEEK

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DNA topoisomerase 1

736 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

83.263 kDa

MKHLIIVESPAKAKTIKNFLDKNYEVVASKGHVRDLSKFALGIKIDETGFTPNYVVDKDHKELVKQIIELSKKASITYIATDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILNVLKTPRKIDMFKVNAQQARRLLDRIVGFKLSSLIASKITKGLSAGRVQSAALKLVIDREKEIRPFKPLTYFTLDALFEPHLEAQLISYKGNKLKAQELIDEKKAQEIKNELEKESYIISSIIKKSKKSPTPPPFMTSTLQQSASSLLGFSPTKTMSIAQKLYEGVATPQGVMGVITYMRTDSLNIAKEALEEARAKILKDYGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALKDYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKGEFKASGRKLLFDGHYKILGNDDKDKLLPNLKENDPIKLEKLESNAHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISLLQNRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQVLKDFYYPFMDKIEAGKKNIISQKVHEKTGQSCPKCGGELVKKNSRYGEFIACNNYPKCKYIKQTENANDEAKQELCEKCGGEMVQKFSRNGAFLACNNYPECKNTKSLKNTPNAKETIEGVKCPECGGDIALKRSKKGSFYGCNNYPKCNFLSNHKPINKRCEKCHYLMSERIYRKKKAHECIQCKERVFLEEDNG

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DNA topoisomerase 1

736 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

83.196 kDa

MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDHKELVKQIIELSKKASITYIATDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILNALKTPRKIDMSKVNAQQARRFLDRIVGFKLSSLIASKITKGLSAGRVQSAALKLVIDKEREIKAFKPLTYFTLDAYFESHLEAQLISYKGNKLKAQELIDEKKAQEIKNELEKESYAISSIVKKSKKSPTPPPFMTSTLQQSASSLLGFSPTKTMSIAQKLYEGVATPQGVMGVITYMRTDSLNIAKEALEEARNKILKDYGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALKDYLKPEELRLYTLIYKRFLASQMQDALFESQSVVVACEKGEFKASGRKLLFDGYYKILGNDDKDKLLPNLKENDPIKLEKLESNAHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISLLQNRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQVLKDFYYPFMDKIEAGKKNIISQKVHEKTGQSCPKCGGELVKKNSRYGEFIACNNYPKCKYVKQTESANDEADQELCEKCGGEMVQKFSRNGAFLACNNYPECKNTKSLKNTPNAKETIEGVKCPECGGDIALKRSKKGSFYGCNNYPKCNFLSNHKPINKRCEKCHYLMSERIYRKKKAHECIKCKERVFLEEDNG

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DNA topoisomerase 1

731 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

82.441 kDa

MEVRGLQPKRQKTFARKHLVIVESPAKAQTIEKYLGTQYVVRASMGHVIDLPKSRLAIDIEHDFQPEYITVRGRAQCLKELRTLSKQSLQVFLASDRDREGEAIAYHLAQSIQAYCDTPIKRIVFNEITPHAIRAAIGHPVPIDTAKVNAQKARRVLDRLVGYHLCPLLWHKVKNGLSAGRVQSVALRLICEREVEVKRFVPEEYWTVEGTFEKDKKSFSALLILIQGKKAVFKSKQEATSAIGLFSQSEARVSQIRSFEKNVRPKQPFTTSTLQQCAANRLGFTSRKTMQVAQQLYEGVSLGTHRVGLITYMRTDSVRVSEAAVKEVRAWIATHFSDALPGTPNRYAAKGKSQDAHEAIRPTYVAHTPERIKAHLTRDQIRLYTLIWERFVASQMTDARVRSLTFEITAGPAVFSATETQVIEQGFYRVLKMLSPKDLSKAVLPPTKEGEVVALHNVQSVQHFTQGPVRYTDASIVKMLEEKGIGRPSTYAPTISVLLDRYYVTRIQKQLMPTPLGKVISDLLTTYFHDVVDVSFTARMESKLDEVEEDKIKWNCVIADFYPAFSEKVSTVMKDLNSMRGVFDEKTDVVCSQCGDTMVKKLGRFGFFLACGKFPECRNTQPVPLAKCPRPACDGNIVGKKTRGRKEFYGCTRFPVCDFVTHFKPTFAVCPQCRCFLVEKSNRRVGTYTACVNPECRYVSPTRKNLSLGKEAVLDGSHRGAQDKGAVQHYE

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DNA topoisomerase 1

719 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

82.773 kDa

MTNHSSKILVIESPNKVKTIKKYLTDDYEIVATVGHIRDLPKYTLGFNTEDFVPKWEIIQEKKPAKGVSATKKAKTVKKAVKNKKEIIDELVDKAKKADEIYLATDPDREGEAISWHVYDVLKEKGVNVNKCKRIIFNEISEKAVKNAIANPREIQKDWVSSQITRRRLDRLIGFKLSSLMKSKINADSAGRVQSIALKFITEREELINKFVPRFWWTLDVILKDGTELMLRKIDEKLKDKLGFKELEEVSGIDFNTKEDAELVKQHLSDKYRVYAIDTPKLKSSYPKEVYKTSTLQQDAINKLKWRSMKITSVAQELYEGVTIDDEQIALISYPRTDSTRLSPEYGKTVLDFVAKTYGKDYVATQSQLNGETKANKKQKAKVQDAHEAIHPIDISITPDSVKNKISSDQYSLYKLIWTRTVAHYMAAAVYELVNIRFINNNNKFYAVNNTLKFDGYKKIYTHYDDKDHLRKLDLNHFAIDKEFEAKDVLLNEHQTKPPARFTQATLIEALETEGIGRPSTYSTILDIVLKRNYAELVNGRYYKTTDLGQKLAFELDKNFPTIINKEFTKNMETTLDEIAQGTVNDVSYLQAFWNDFSEVLNEAKTNIVKKVEYIEGKNCPNCSSQLVRREGRYGPFVGCSNFPKCKFIEKSEQKAKEIVYEEGVKCELCHAKLIRRVAKKKGRNKDNTFLGCSNFPKCKYTKSLEPEKDETIRDSTII

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DNA topoisomerase 1

718 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

81.521 kDa

MRSLSGKMHEVIICEKPKSSEKIAGALFPDAMKKKHGKVSYWEHVEGDKRVTIVSAVGHLYSLRPRQSNEEHFFDLEWAPIHEIDKKKGYVKDYLNVIRKFAAGADRYIHACDYDIEGTLIGFNALKYGCGEEALRKTSRMKFSTLTREEIQRAYKNPIEVDYGQVDSGAARHILDFIFGVNISRSLMKSVKAATNRFIKLSAGRVQTPTLAILVEREKEIRDFKPVPYWIIRAELGEGIIAESKRGKIFKRELVDSILEKCQGSDAEVKDVRVRDTIRKPPVPFDLGTLQSEAYRVFGFSPKKTQTIAQNLYTEGYTSYPRTSSQKLPESIGYEKILKNLAKNPRFGVHIERLRGPLKPHEGKKEDDAHPAIHPTGLLPSELSKDEKKVYDLIVHRFISVFGEDAILQTMKVELEIGEEEFSFSRKRVSKAGWMESYPYTKMEDEEFPEISGGDSLAVRSVSADERETKPPARYNEASLIRELERRGLGTKSTRADIIAKLYDRKYIEGKKIRVSPLGENIIDTLTRYCEKIISEELTRQFERELEDIMRGKISKDRVIDEAITEVRSILSDIEENLRDIGKELYRAYQDSRVVGECPACGGKLVIKYSPRNRSTFVGCSSYPDCRTVYSLPRGASVLKSLCEKCGLPMISYGRPRQRACLDPKCGKKKSEVEEVVGKCPECGSDLIKRSGRYGEFVGCKGFPKCRFTCSVDEVPEG

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DNA topoisomerase 1

711 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

81.965 kDa

MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWEDWTKSKKKFSSLSFKGNLKGKKLLSKYDIIKSIKEKASKATNIYLATDPDREGEAISWHVYDVLDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFWWTLDVLLNPKAEGVREACANRSIPIVLREINPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINITPEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSKHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS

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DNA topoisomerase 1

710 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

80.741 kDa

MPTSTKSKTKTTTKKKTTRKRVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKKSTMSVDIENDYEPQYINIRGKAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQARRVLDRLVGYSISPILWKKVKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHPLKTNEDVIKVMERLDGPDFNVDKVEKKERRRNAPLPYTTSSMQQDAANKLNFRTRKTMMVAQQLYEGLTLGSQGHQGLITYMRTDSTRISPVAQSAAHNYIADQFGEKYSKHGSKVKNASGAQDAHEAIRPSNVFNTPDAIAKYLDKDQLKLYTLIWNRFVASQMVAAIFDTVKVNLSQNGVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENETVKKANVKPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLETIQKRYYVRLSAKRFEPTELGEIVNKLIVEFFPNIVNTEFTAEMEKDLDEVEEGKRQWIEVVDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEGNIIERKTKKNRIFYGCDRYPDCEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK

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DNA topoisomerase 1

709 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

82.545 kDa

MIKNLVVIESPNKVKTLKQYLPSDEFEIVSTVGHIREMVYKNFGFDENTYTPIWEDWTKNKQKNPKQKHLLSKFEIIKSIKAKASDAQNIFLASDPDREGEAISWHVYDLLDQKDKAKCKRITFNEITKKAVVDALKQPRNIDLNWVESQFARQILDRMIGFRLSRLLNSYLQAKSAGRVQSVALRFLEEREKEIAKFVPRFWWTVDVLLNKENNQKVVCANKSIPLVLREINPELSASLKLDFEAAENVSGIDFLNEASATRFANQLTGEYEVYFIDEPKIYYSSPNPVYTTASLQKDAINKLGWSSKKVTMVAQRLYEGISVNGKQTALISYPRTDSIRISNQFQSECEKYIEKEFGSHYLADKNKLKRHKKDEKIIQDAHEGIHPTYITITPNDLKNGVKRDEFLLYRLIWIRTVASLMADAKTSRTIVRFINQKNKFYTSSKSLLFDGYQRLYEEIKPNTKDELYIDLSKLKIGDKFSFEKISVNEHKTNPPPRYTQASLIEELEKSNIGRPSTYNTMASVNLERGYANLVNRFFYITELGEKVNNELSKHFGNVINKEFTKKMEKSLDEIAENKVNYQEFLKQFWTNFKSDVKLAENSIQKVKKEKELVERDCPKCNQPLVYRYTKRGNEKFVGCSDFPKCKYSEFSNPKPKLTLETLDELCPECNNKLVKRRTKFNAKKTFIGCSNFPNCRFIKKDNAAEFKQ

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DNA topoisomerase 1

707 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Belongs to the type IA topoisomerase family.

80.296 kDa

MYAILAEKPSAAKAYAQALNGKRQGRIYVAPPSSLLPEGALICAAVGHILEFLEPGELNEKYKSYSLDSLPIIIDLFQYKVVSDKKEVLQRIKDTIFDKRVKTIILATDAAAEGEYIGRNILYRLQCKKTIKRLWTSSMTATSIQKAFSQLKADAETLPLYYQAKARAESDYMIGLTLSRAYGILLKEQGIVPHNTTISLGRVQTPLLAEIVKRERLIEQFTAENFWTVKATFNNQGNVYEGEWFHEKENRIFKEEQAEQLCELVRNQSSTIMEMKEEMRTYQPPLLYXLSTLQMDAGNAFGFKPAETLKYAQSLYDKGYLSYPRTQDERITESDARELENNIQFLSGHDTFGALFPLPVSTLMNNKRYIGEVTDHHALLITDKIPKDKDLSEDEKSIYHLVVKRILAAHYPDVAMSHKEIITKVMDRFTFRSKGKELLSKGWHHIIPPTNENDIMLPTLLKGSEGVVTDTLTTKSKTKPPNRYTSSSLIGFMKNAAQAIEDEDRKSISNLPLGTEATRAGLITLLESRKYIEWKKNKVYPTLLGITVVDSIKRGSVIKSPILTAKWDVKLNEIGASLYNHKDFIAHSKKLSSVLFEEVKTYSSTWNQNGVERIKSESIGACLLCGSNVVLRKGKHGEFYGCSNYKDSGCTFNLPFKVLNKKLSKKQLMELLKNEKTDIIKGFKWKDKTFNAPLVWNREDQKVQFGK

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DNA topoisomerase 1

700 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.148 kDa

MKKNLIIVESPAKAKTIGNFLGKDYEVIASKGHIRDLPKSSFGIKIEDDEFIPEYRITSDHSALVKELKSKAKDAKEVYLATDEDREGEAIAYHIAKAIGKDENTLPRIVFHEITKSAIENALKNPRKLNMHSVNAQQTRRLLDRIVGYKLSPLLGQKIQRGLSAGRVQSAALKIIVDREKEIRAFVPLEYFSIDMIFQKDLDAELVEFDKAKIEKLTITNKDRAKLILEACKNEVYSISDIESKERKIAPPPPFMTSTLQQSASNRLGFNPKKTMMIAQKLYEGVNTHEGVMGVITYMRTDSLNLAKEAVENARKFIQANFGKDYLPSKANVYTTKTKGAQEAHEAIRPTNLSFTPEIAAKFLDKDELKLYTLIYNRFLACQMSPAISQTQNVFVKNDRVVFKISGRKILFDGYYKVYGDMDKDKILPNFKIGQNLKVQNLEMNSHFTEPPSRYSEAGLVKKLESLGIGRPSTYAPTISILTSRDYVTIDKKQLIPSDVAFNVTEVLEKNFSDIVDSKFTSNLENTLDEIAEDKADWQETLKEFYYPFMRKIEEGKTKIASQKTVTKLGESCPDCGGELAIRKGRFGEFVACLNFPKCKYSRNLKSESKNESENTAAKAKANGTGITCPSCQKGEIVERFSKRGKFYGCSAYPKCNFISKYKPSEEKCEECGETLVIKELKKGTFLECLKCKIKKEMKD

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DNA topoisomerase 1

699 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.612 kDa

MAKSKVVEKDKKNELDNQSADIELKGQSKNEESKGGKKKVIIVESPAKAKTIERILGNDYQVISSKGHIRDLPQKQFGVDLNSLKLDFEIIPGKESVVEQIKKMTSGKEVLLPSDQDREGEAIAWHLSTILGVKGKNIITFTEITERAIKEAVKNPREIDMNKVNAQLARRVLDRIVGYMISPLLWRIIKDARSAGRVQSAALKIICERERERYRFVPQKYFKVWIDIAGLKAYLTKVDGKKIKPTDITEEISKDVLQKVKSVRLVDIDVKEVRKNPPAPFITSTLQQDAASKLGFPVSKTMKIAQELYEGVDTKEGHTAFITYMRTDSTRVSDVAKEAAEKFILKNFGREYLNESSEVASKKGSKTKGKVQDAHECIRPVDVNITPEKAKELLDKDHHKLYELIWKRFIASQMSSAIYKQYSYDFESGKYVFEASIRERIFDGFEKVYTIDNEPSEKHKELKVDQEYLVEPKSAEAQTTPPDRYTEASLVKTLEMEGIGRPSTYATIIQTLLDRGYVVKKRKTLIPTILGFVVNHYLEQRFPDIVDKGFTAEMEKELDEVENGKKDWKEVVKSFLKEFNKDLERAKNEFFAIDFDTDITCEDCSGNYKLKVGKYGLYLHCPNCKTNKALKSDVFGVIDGNKLYVLEEQESQEENGEKNSVQSEESSANSGNRKFYRKRRTSGSKKSSTKSASSKAKKK

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DNA topoisomerase 1

691 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.078 kDa

MSDYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGKGPVLKELKTAAKKAKKVYLAADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKESFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKKGLSAGRVQSVALRLIIDREKEINDFKPEEYWTIDGTFLKGQETFEASFFGKNGKKLPLNSEADVKEILSQLKGNQYTVEKVTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGREGTVGLITYMRTDSTRISNTAVDEAAAFIDQTYGKEFLGGKRKPAKKNENAQDAHEAIRPTSVLRKPSELKAVLGRDQMRLYKLIWERFVASQMAPAVLDTMSVDLTNNGLTFRANGSKVKFSGFMKVYVEGKDDQMEEKDRMLPDLQEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEERGIGRPSTYAPTLDTIQRRGYVALDNKRFVPTELGQIVLDLIMEFFPEIINVEFTAKMERDLDHVEEGNTEWVKIIDNFYTDFEKRVKKAESEMKEVEIEPEYAGEDCELCSSPMVYKMGRYGKFLACSNFPDCRNTKPIVKQIGVKCPSCGEGNIVERKSKKKRVFYGCDRYPDCEFVSWDKPIERKCPKCGKMLVEKKLKKGIQVQCVECDYKEEPQK

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DNA topoisomerase 1

690 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

78.911 kDa

MADYLVIVESPAKAKTIGKYLGKKYTVKASMGHVRDLPKSQMGVDIEDEYSPRYITIRGKGPVLKELKSAAKKVKRVYLAADPDREGEAIAWHLAHSLDIDETSECRVVFNEITKQAIKDAFKSPRPINMDLVDAQQARRVLDRLVGYNISPLLWKKVKKGLSAGRVQSVAVKMIIDREKEIQAFEPEEYWSIQGTFALDGEPFEAKFYGTNGKKVELKSEDDVNNVLAKLKGDQFHVDSVQKKERKRNPVSPFTTSSLQQEAARKLNFRAKKTMMLAQQLYEGIDLGKEGTVGLITYMRTDSTRISDTAKAETKEYIESKFGKEYAQNGTKAVKKDKKSQDAHEAIRPTSVERDPKSLKEYLSRDQLRLYKLIWERLVASQMAPAIMDTMTVDLSNEGVLFRATGSKVKFPGFMKVYIEGNDEGKKEEDRLLPNLQEGQTVTKEEIEPSQHFTQPPPRYTEARLVKTMEELGIGRPSTYAPTLDTIQKRGYVALDERRFVPTELGEIVLELMTEFFPEILDVEFTAKMEDDLDSIEEGKQQWIKIIDDFYKGFEERLKVAEEEMKEVEIKDEPTGEMCEKCGHEMVYKMGRYGKFMACSNFPDCRNTKPIVKEIGVKCPKCEKGEIVERKSKKRRVFYGCNQYPECDFVSWDKPIARSCPKCSSYLVEKRTKKQVQVQCSSCDYKEEAN

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.113 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.113 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.123 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWIIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDIEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDIDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.113 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.071 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.071 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.071 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.071 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.1 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITTALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITNKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.071 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.519 kDa

MADNLVIVESPAKAKTIEKYLGKRYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKDLKKHAKKAKKIFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKDSFKHPRGIEMDLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWSIEGEFRYKKSKFTAKFLHYKNKPYKLNNKDDVQRITEALNGDQFEITNVNRKEKTRYPAHPFTTSTLQQEAARKLNFKARKTMMLAQQLYEGIDLKRQGTVGLITYMRTDSTRISTSAKSEAQQYINDKYGEQYVSQRKSSGKQGDQDAHEAIRPTSTMRTPDDMKAFLTRDQHRLYKLIWERFVASQMAPAILDTVALDVTQNDIKFRANGQTIKFKGFMTLYVEAKDDKENDKENKLPQLDKGDKVTATKIEPAQHFTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFIPTELGEIVYEQVKEYFPEIIDVEFTVNMETLLDKIAEGDMNWRKVIGDFYNSFKQDVERAESEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKTIGVTCPKCNEGDVVERKSKKNRIFYGCSRYPECDFISWDKPVGRDCPKCHHYLVNKKKGKSSQVVCSNCDYEEEVQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.519 kDa

MADNLVIVESPAKAKTIEKYLGKRYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKDLKKHAKKAKKIFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKDSFKHPRGIEMDLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWSIEGEFRYKKSKFTAKFLHYKNKPYKLNNKDDVQRITEALNGDQFEITNVNRKEKTRYPAHPFTTSTLQQEAARKLNFKARKTMMLAQQLYEGIDLKRQGTVGLITYMRTDSTRISTSAKSEAQQYINDKYGEQYVSQRKSSGKQGDQDAHEAIRPTSTMRTPDDMKAFLTRDQHRLYKLIWERFVASQMAPAILDTVALDVTQNDIKFRANGQTIKFKGFMTLYVEAKDDKENDKENKLPQLDKGDKVTATKIEPAQHFTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFIPTELGEIVYEQVKEYFPEIIDVEFTVNMETLLDKIAEGDMNWRKVIGDFYNSFKQDVERAESEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKTIGVTCPKCNEGDVVERKSKKNRIFYGCSRYPECDFISWDKPVGRDCPKCHHYLVNKKKGKSSQVVCSNCDYEEEVQK

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DNA topoisomerase 1

689 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.373 kDa

MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDVEDNYEPKYITIRGKGPVVKDLKKYAKKAKNVFLASDPDREGEAIAWHLSKILELDDSKENRVVFNEITKDAVKESFKHPRGIEMDLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWSIEGEFRYKKSKFTAKFLHYKNKPFKLKTKKDVEKVTAELDGDKFEITNVNKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMLAQQLYEGIDLKRQGTVGLITYMRTDSTRISQTAKDEAKQYIEDKYGKDYLSNRTAKGKQGDQDAHEAIRPTSTLRTPYEMKAYLTRDQHRLYKLIWERFVASQMAPAILDTVALDVTQNNIKFRANGQTIKFKGFMTLYVEAKDDKDNEKENKLPNLSKGDEVTATQIEPAQHFTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEIVYEQVKDYFPEIIDVEFTVNMETLLDKIAEGDIGWRKVIDNFYGSFKLDVERAEEEMEKVEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKTIGVTCPKCKDGDVVERKSKKNRLFYGCSNYPECDFISWDKPVGRDCPKCNHYLMEHKKGRSSQVICSNCDYKEEVQK

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DNA topoisomerase 1

688 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

79.13 kDa

MAENLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDAENDYEPKYITIRGKGPVVKELKKHAKKAKKVFLASDPDREGEAIAWHLANILNLEDSTENRVVFNEITKDAVKDSFKHPRGIEMELVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWKIEGEFRYKKTKFTAKFLHFKNKPFKLTEKADVEKITTQLDGDQFEVTKVTKKEKTRYPANPFTTSTLQQEAARKLNFKARKTMMLAQQLYEGIDLKKQGTVGLITYMRTDSTRISDQAQSEAKNYIQETYGNDYTSNRKSKGQGDQDAHEAIRPSSTLRTPNEMKNFLTRDQHRLYKLIWERFVASQMAPAILDTVAMDLTQNDIKFRANGQTIKFKGFMTLYVETKDDSEDGKDNKLPNIGEGEMVTATNIEPSQHFTQPPPRYTEARLVKTMEELKIGRPSTYAPTIDTIQKRNYVKNESKRFVPTELGEIVHEQVKDYFPEIIDVDFTVNMETLLDKVADGEIGWKKVISDFYSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKTIGVTCPKCKEGDVVERKSKKNRIFYGCSKYPECDFVTWDKPIGRDCPKCEHYLVEKKQGRKSQVVCSNCDYKEEEQK

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DNA topoisomerase 1

686 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

78.637 kDa

MILIIAEKPNVARKIAGALSERRPIKKSLFGVPYYEIFREGKKLIVASAVGHLYGLAPKRDVFGYPIFDIEWVPVYIAEKGKEYAREYIKALSVLAKRVREFIVACDYDTEGEVIGYTALKYACGVDPRVAKRMKFSALTKRDLLNAWRNLEPTINFGMANAGIARHILDWYWGVNLSRALTHAIKKASGKWVVLSTGRVQGPTLKFLVEREREIQSFVPRPYWVIKLIFEKNGQKFTANYEKDKIWEEEEGKRIVLEVKKSIPRVSNVEIKRQKRTPPHPFDLGTLQREAYSAFGFSPKKTLDIAQSLYEKGFSSYPRTESQKLPRNINFRMIIQNLAKMPQYRPYAHILLGLPELKPVEGKKEDPAHPAIYPTGEIPRPGDLTKDEEKLYDMIVRRFLAVFMEPAIRESVKVTIRAGPHKFFLSGGRTVKKGWLSVYGKYVKFEEVTLPEFFIGERIKVIQVKREKKKTKPPARYSPAAVIKKMEDLGLGTKATRAQILETLYQRGYIEGKKSIKVTPLGMKVIETLEKYVPEIISVELTREFEKKMELIMEGRLTKEEVIEEAKERLTKILEEFKKRELEIGIELAKIVVGEDEVKPLVVGKCPKCGGDLIVKYNKKTGKRFVGCSNWPKCDVTYPILQRGEIIPTNKTCCNGAPVVIIREEDGREFEICLDINCKDWKAKSH

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DNA topoisomerase 1

685 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

78.196 kDa

MILVIAEKPNVARKIAGALSERRPIRKTIFGVPYYEVFRDGKKLIVASAVGHLYGLAPKQDFFGYPIFDIEWVPVYIAEKNKDYAKDYIKALSVLAKRVKEFIVACDYDTEGEVIGYTALKYACGVDPSRAKRMKFSALTKRDLLNAWRNLEPTINFGMANAGIARHVLDWYWGVNLSRALTHAIKRASGKWVVLSTGRVQGPTLKFLVEREREIQSFVPKPYWVIKLVIEKNGQKLVANYEKDKIWSEEEAKKIVTEVKKSKARVSNIEVKRQKRNPPVPFDLGTLQREAYSAFGFSPKKTLDIAQSLYEKGFTSYPRTESQKLPKNLNFRLIIQNISRMPQYRPYAHLLLGMPELKPVEGKKEDPAHPAIYPTGEIPGPGDLSKDEEKLYDMIVRRFLALFMEPAVRESVKVTILAGPHKFMLSGARTVKQGWLSVYGKYIKFDEVTLPEFFIGERVRVLQVRREKKKTKPPARYSPAAVIKKMEDLGIGTKATRAQILETLYQRGYIEGKKSIKVTPLGMKVIETLEKYVPEIISVELTREFERKMDLIMQGKLTKEEVIEEAKVKLTKILEEFKKRELEIGLELARIVVGDVKDEIKEEKPIVVGKCPKCGGDLIVKYNKKTGKRFVGCSNWPKCDVTYPILQRGEIIPTNKTCCNGAPVVIIRDKGRDIEICLDIKCKKW

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DNA topoisomerase 1

673 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

75.571 kDa

MVAEKPKAAAKIAYALSDGRGVLRCSEYGVPYWIVRRDGAAIVVAPSAGHLFGPHTDSRGFPVFDFEWRPIFEFDRGAGYLSKFYRMLSRILPGASLYVNACDYDIEGSVIGFKIIEAFGDVNRARRMKFSTLAPQDIRRAYARMERLDVEMIEAGMARSEMDWLWGINVSRALMEAARRAAGRRVILSAGRVQSPTLVEAYRRWREINLHVPKASVAVKITAEKGGGVFDARPHGWKPQSLETARSIKSELRKNPWLAVEEVRSERSILRPPPAFNLGDLQKEANRILGLPPLRTQSIAEELYLEALISYPRTNSQKLPPSINYRAILDKLAHGPLGREARELLKETGGVLRPVQGSKDDPAHPAIHPTGEKPSQRLSKEHMAVYELIVRRFLAAFSREAIVSKSSVLLRDFQGRVWRAEGLRVEDLGWLKYYHYSTPGEKPMPPLDRGDKARVVRVDVRVEWSQTPVRLDKASLLRWMESVNIGTEGTRARIIETLYKRGYLEGSRKSEVTPLGEAVAVIIQTLFPELSKPDLTRRFESMIEDIRSGRRTRQEVIDMSKKTISKLLESFLDRLDTAVREIGVSLGSVEVEAACHLCGRKAVSAVSGYRLCSHHMEAFDRLRKALPNLASTISSTPREALEAIARGRSRAGAWVRDVAALALRDDGLYKALL

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DNA topoisomerase 1

663 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

76.018 kDa

MVMSWLIITEKDNTARRIASILFKDVKTLKKGRVSYYHSPSNDAYVVGLKGHIVELDFPKELNNWTKTPLEKLLQAELVKKVKERTISSILKEIAKKADRVTVATDYDREGELIGVEALEIVKSVNPTVKVDRVRYSAVTEKEIRSAFSKPVKVDFNLANAALARQKIDLIWGATLTRLISVHSGRMGKDFLSVGRVQTPTLRLIVDRELEIQNFKPEKYYEIFAEFEGFIAKHPKRYGSKEEAEKLFAKIGETAKVEEFTRRRMEENRPTPFNTTEFLREASKFMSPHKAMNIAETLYMNGYISYPRTDNTVYPPTINLIEIVSALSSVFPREAQIVLSQDKITPSRGRRETKDHPPIYPTGVAKRGELSKDEWTIYELVVRRFLATLAPKAVWDVRRVVLDSNGVKFVANGRQLVEAGWRDIYIYSKAEETELPLLKKGDVLRILKKRLEEKETKPPGRYSASSLIKMMEKLNLGTKSTRHEIIQKLVSRRYIHGNPFRPSETAFSVINVLKETAETITLPDMTAKLENEMDLIAEGKKREPEVVDESREMLLQILRAIDYRKLSKDLREGVKKDKIVGKCPECGGELVVRQSKAGKRFIGCSNYPDCTFTLPLPQNGTLYITAKQCKEHEIKEVKIRTKKGYWNLGCPYCNYLNWKKENS

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DNA topoisomerase 1

633 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

72.695 kDa

MSKKVKKYIVVESPAKAKTIKSILGNEYEVFASMGHIIDLPKSKFGVDLEKDFEPEFAVIKGKEKVVEKLKDLAKKGELLIASDMDREGEAIAWHIARVTNTLGRKNRIVFSEITPRVIREAVKNPREIDMKKVRAQLARRILDRIVGYSLSPVLWRNFKSNLSAGRVQSATLKLVCDREREILRFVPKKYHRITVNFDGLTAEIDVKEKKFFDAETLKEIQSIDELVVEEKKVSVKKFAPPEPFKTSTLQQEAYSKLGFSVSKTMMIAQQLYEGVETKDGHIAFITYMRTDSTRVSDYAKEEARNLITEVFGEEYVGSKRERRKSNAKIQDAHEAIRPTNVFMTPEEAGKYLNSDQKKLYELIWKRFLASQMKPSQYEETRFVLRTKDGKYRFKGTVLKKIFDGYEKVWKTERNTGEFPFEEGESVKPVVVKIEEQETKPKPRYTEGSLVKEMERLGIGRPSTYASTIKLLLNRGYIKKIRGYLYPTIVGSVVMDYLEKKYSDVVSVSFTAEMEKDLDEVEQGKKTDKIVLREFYESFSSVFDRNDRIVVDFPTNQKCSCGKEMRLSFGKYGFYLKCECGKTRSVKNDEIAVIDDGKIFLGRKDSESGSPDGRSVEGKGNLSEKRRKGKKGS

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DNA topoisomerase 1

540 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Belongs to the type IA topoisomerase family.

63.427 kDa

MELFIVESPTKAKTIQKFLGKGFLVKATLGHVKDLPEKELGVDLRTLKAKYVYKRGKKKLVEQLKKLSRRSSIVYLGTDPDREGEAIAYFLKKDLEKVNKNIKRAVFYEITPEAIRESIRNAGDVNMNLVYAQFARRILDRLIGYLISPILWKEFKNYKLSAGRVQSPALRLIVEREREIQNFKVKKYYYVKALLRKGSEEFWAIYDYRYENPSDAKIIAKKLEKGYFSVYKVEKKKEKVSPPKPFITSDLQSEANAKFGFSSERTQKLAQELYEKGYITYPRTDSYRMNEKKAKEFMNYIEKKYGKEYVGRLRRFREKATAQGAHECIRPTSLREEIPEREELRLLYDLIFRRTIASLMKEMLLEREKVTVEAITPELKHPVYLVAKGLKIVFDGWSRVYPSEITEEKLPELYEGDLLDLVKTTLEERKTQPPPRYTEGTLIKTLEKLGIGRPSTYATIVKTLKERGYVEVKKKALVPTEIAFQVVEFLMERFPTLMDYKFTAQMEEKLDLVEEGKLNWKSVVYEFMEKIFGKELEMVR

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DNA topoisomerase 1

18 amino acids

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Belongs to the type IA topoisomerase family.

2.043 kDa

single

ATGWSAFFIDGKWTEAKK