Protein
Probable tRNA sulfurtransferase
Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Function
Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.
Similarity
Belongs to the ThiI family.
Sequence
MNEDYILIRFGEISTKGKNRKLFVDRLKRNIKMVLRDFRNVRYESTRDRMTLVLNGEDAEAVMARLKHVFGIQSFSLAVKCRTDLESIKETALASVQEQYKPGDTFKVSTKRAYKQFELNTNEMNAEIGGHILRNTDDLTVDVHSPDIHLRIEIREDATYLTFRDEKGAGGLPVGSGGKAMLMISGGIDSPVAGFYAMKRGLEIEAVHFFSPPYTSERAKQKVIDLTKRLTAFGGDIKLHIVPFTKTQELIQKQIPENYSMTATRRLMLQIADKLRERHNALAIFTGESLGQVASQTLESMYAINAVTSTPVLRPLIGMDKTEIIEKAKEIDTYDISIRPYEDCCTIFTPSAPKTRPKKEKIEHFESYTDFEPLINEAVENTETIVLSSKAETKDQFADFF