Protein
tRNA-guanine(15) transglycosylase
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Function
Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs. Can also utilize guanine as substrate.
Similarity
Belongs to the archaeosine tRNA-ribosyltransferase family.
Sequence
MTFEIKHRDAMGRIGILNINGKKIETPTIMPVIHPNPKKQIVSMDLINKLADVIITNSYITYKTKHLREIAEEKGIHKLIGFDKVIVTDSGSFQLGVYGDVEVEPMEIIEFQERIGVDVGTILDIPTPPDVDRERAEKELEETLKRAKASIELKEERGFKLLLNGTVQGSTYLDLRQKSAKEMAKLGFDIYPIGAVVPLMEQYRYRDVAEIIINSKMYLPTNKPVHLFGCGHPMFFALAVALGCDLFDSAAYALYAKDDRYLTERGTLHLEEIKDLKAFPCSCPVCSSYTPKELASLNKKERERLLAEHNLYVTFEEINRIKQAIRDGSLWELVEERVRCHPKLLEAYRVVRKYIDYIEKFDPVTKKSAFFYTGIESMFRPEVLRHKKRLKRLRYEKVYITTVSSSIEKPYHEHLNVVETDVDILIKDPVFGFIPYYIDTVYPLSQHEIPELFDYEKEINKRFVDEFIDWLKKKIGEDNILDIMTYNYYINYFSANKKINADALRIRKMLQYQYGFDIIDDELMNKIKVVRSKTTGRLRQVLDENGEILFSVRSNDNLLIPSEKGAKLLWKKIPFPKYRVVVNKEAEEFAREGRNVFAKFVIDCDEELRPYEEVLVVNEDDELLAYGTTILNGIELREFNYGLAVKVRGGLKINK