Protein
(E)-2-((N-methylformamido)methylene)succinate hydrolase
Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Function
Involved in the degradation of the pyridine ring of trigonelline (TG; N-methylnicotinate) into succinate and methylamine as carbon and nitrogen sources, respectively. Catalyzes the hydrolysis of (E)-2-((N-methylformamido)methylene)succinate (MFMS) into formic acid, succinate semialdehyde (SSA), methylamine and carbon dioxide.
Similarity
Belongs to the AB hydrolase superfamily.
Sequence
MISKTLQLSNNRTAHYFEQGEGEPLVLIHGVGMQAEAWYPQIEYFSKHYHVISLDMPGHGQSTALAADAQLQDFVDWAIECIHTLNLGPVNLAGHSMGSLITTGVSVTRPDLVKRMAVLNGVYKRTHAAREAVIQRAEALKQGHLDIETPLQRWFGQSEIEKIASERVKLWLENVNMSGYTTAYRAFAQGDLVYADGWSDIECPALVLTGTDDPNSTAEMTIQMAHQAKHGTAIVIENERHMVNLTAPEKVNQAMQAWLETTP