Function
FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of terrein, a fungal metabolite with ecological, antimicrobial, antiproliferative, and antioxidative activities (PubMed:24816227). The first step in the pathway is performed by the polyketide synthase terA that produces 4-hydroxy-6-methylpyranon (4-HMP), orsellinic acid (OA), and 2,3-dehydro-6-hydroxymellein (2,3-dehydro-6-HM) by condensing acetyl-CoA with two, three, or four malonyl-CoA units, respectively (PubMed:24816227). 4-HMP and OA are not pathway intermediates, but are rather shunt or side products (PubMed:24816227). 2,3-dehydro-6-HM is further converted to 6-hydroxymellein (6-HM) by the 6-hydroxymellein synthase terB (PubMed:24816227). The monooxygenases terC and terD, the multicopper oxidase terE and the Kelch-like protein terF are then involved in the transformation of 6-HM to terrein (PubMed:24816227). Even if they are co-regulated with the other terrein cluster genes, terH and terI seem to be dispensable for terrein production; whereas one or both of the 2 transporters terG and terJ are probably required for efficient secretion of metabolites (PubMed:24816227).
Sequence
MSSKFDVVICGSGTAGLAAATWLAQYGVDCKILESRSGPLDVGQADGIQVRSVEIFESFGMAEELLREAYHNIEVAFWGSNPTSSGMGIVRKRSAHATTPGLSHMPRVILNQARFNEMWLEAMRRRNGQEVDYGHKVTKVTVDEEKATDPDAYPVTIVAQKDGQEQVFEAKYCLASDGAHSAVRKSLGFNMVGETSDSVWGVMDVFPRTNFPDIRKQCIIQSDAGSIITIPREGGSMVRVYVELAAGTNAKEVTLEQIQNASRQVFHPYALDVADVWWWSAYPIGQRIADHFSKANRVFLTGDACHTHSPKAGQGMNVSLQDGYNIGWKLASVLKGHAGPELLETYVLERQKVADVLINWDKVWAKQMCSIAKEDGGVVDANGKIDFSEVFVKAEAFTAGLTVTYGDSIITQAGDSNQQAATNLKVGMRLPAAQVVRFCDAKVMKTVNALPSDGRWRIMIFPGDIRQPSASTRLAQLGTYLFSNHGPIRKYLPPGADIDSLIEVIVILSGERLEIQQDQIPDAFWPTTGKYRMRDLHKIYIDDETYHNGHGHAYDFYGIDPERGAVAIVRPDQYISKVLDMKNHEGISAFFEKFLQKKGQANGSLNSHDEWTLA