Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Function
Transpeptidase that anchors surface proteins to the cell wall. Recognizes Leu-Ala-x-Thr-Gly and Leu-Pro-x-Thr-Gly, with a preference for the former. Unlike the S.aureus sortase it cleaves not only the Thr-Gly motif but also the Ala-X bond; an Ala-Glu bond is a better substrate than the Thr-Gly motif in vitro. Among its possible substrates are the chaplins ChpA, ChpB and ChpC; this enzyme is more important for ChpC attachment than is SrtE1. A double knockout mutant of srtE1 and srtE2 shows a developmental defect in aerial hyphae formation more dramatic than that due to chaplin deletion.
Similarity
Belongs to the bacterial sortase family. Class E subfamily.
Sequence
MAATTDTEHQEQAGTGGRGRRRPGRIAAQAVSVLGELLITAGLVMGLFVVYSLWWTNVVADRAADKQAEKVRDDWAQDRVGGSGQDGPGALDTKAGIGFLHVPAMSEGDILVEKGTSMKILNDGVAGYYTDPVKATLPTSDEKGNFSLAAHRDGHGARFHNIDKIEKGDPIVFETKDTWYVYKTYAVLPETSKYNVEVLGGIPKESGKKKAGHYITLTTCTPVYTSRYRYVVWGELVRTEKVDGDRTPPKELR