spuD

Gene

spuD

Protein

Putrescine-binding periplasmic protein SpuD

Organism

Pseudomonas aeruginosa (strain UCBPP-PA14)

Length

367 amino acids

Function

Putrescine-binding protein probably required for putrescine uptake into cells. Binds putrescine with high affinity, spermidine with relatively low affinity. Does not bind cadaverine or spermine. Putrescine binding induces large inter-domain conformational changes.

Similarity

Belongs to the bacterial solute-binding protein PotD/PotF family.

Mass

40.63 kDa

Sequence

MMKRFGKTLLALTLAGSVAGMAQAADNKVLHVYNWSDYIAPDTLEKFTKETGIKVVYDVYDSNEVLEAKLLAGKSGYDVVVPSNSFLAKQIKAGVYQKLDKSKLPNWKNLNKDLMHTLEVSDPGNEHAIPYMWGTIGIGYNPDKVKAAFGDNAPVDSWDLVFKPENIQKLKQCGVSFLDSPTEILPAALHYLGYKPDTDNPKELKAAEELFLKIRPYVTYFHSSKYISDLANGNICVAIGYSGDIYQAKSRAEEAKNKVTVKYNIPKEGAGSFFDMVAIPKDAENTEGALAFVNFLMKPEIMAEITDVVQFPNGNAAATPLVSEAIRNDPGIYPSEEVMKKLYTFPDLPAKTQRAMTRSWTKIKSGK

Protein

Putrescine-binding periplasmic protein SpuD: spuD

Gene

spuD

Protein

Putrescine-binding periplasmic protein SpuD

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Length

367 amino acids

Function

Putrescine-binding protein probably required for putrescine uptake into cells. Binds putrescine with high affinity (KD=3 nM), spermidine with relatively low affinity (KD=6.8 uM). Does not bind cadaverine or spermine. Putrescine binding induces large inter-domain conformational changes.

Similarity

Belongs to the bacterial solute-binding protein PotD/PotF family.

Mass

40.63 kDa

Sequence

Protein

Putrescine-binding periplasmic protein SpuD: spuD