Protein
Modification methylase SfiI
Organism
Streptomyces fimbriatus
Function
This methylase recognizes the double-stranded sequence GGCCNNNNNGGCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the SfiI endonuclease.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.
Sequence
MRKPSSGQPAGLDAWDETRSLATHAGPDFALYVGDSLDCLAKLPDESINTVVTSPPYWAVRDYEHDEQLGLEDEVDDYVERLVKIFREVYRVLATDGSAWLNIGDSYFNKQITVGGKPPRTGWKRNKQLSLVPFRVALALQDDGWWIRNVAVWHKPNAMPASVRDRLTVTWEPVFLLTKSERYYFNLDEIRVPHQTSDAIERRRAESGTVTGKAQGKKELRKWLNSPRHRATIEGIKEVERRPNAPAAVELASYLRTALKEKKRSIAWVAEQLDLPFERTRHYFRTDEIGSRLPPPEVWEQLKDLLELDATYDEAMTVEVGDNVFRNHPNGKNPGDLLSIPTAPSGANHFAVMPRKLAHFALKATLPMNGSCLDPFMGSGTTGRVVRELGGRFVGVDVNEHYMTDYLVESGVISPETETLW