Protein
Modification methylase ScaI
Organism
Streptomyces caespitosus
Function
This methylase recognizes the double-stranded sequence AGTACT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the ScaI endonuclease.
Similarity
Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.
Sequence
MSGRDFGYVIQSSAALWNRLSTFSQRGKALDTRLADIKKALGKPYYETSDVLLYHGDSLELLKSMPQQIFDLTVTSPPYNIGKEYEGVLSIEEYISWCETWMSRVHRATSAGGAFWLNVGYVPVPNQGKAVPIPYLLWDKSPFYMIQEVVWNYGAGVASRKSFSPRNEKFLWYVRDPLNYYFDLDSVRDPNVKYPNQKKNGKLKCNPLGKNPTDVWQFPKVTSGAKRSSVERTAHPAQFPSAVIERVIKACSPSDGVILDPFLGSGTTSLTARKQGRCSVGIEIREDYLDIAVGRLEAEAQSLF