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rpfB

Gene
rpfB
Protein
Resuscitation-promoting factor RpfB
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Length
362 amino acids
Function
Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity (By similarity).
Similarity
Belongs to the transglycosylase family. Rpf subfamily.
Mass
38.271 kDa
Sequence
MLRGVVGAFLVSLTVAGSYAVASHKTVTLSVDGAPMTVTTMKSRVIDIVEENGFEVSDRDDLFPAATETVHQSDTIVLRRSRPLEVSLDGEGTKQVWTTASTVDEALAQLRMTDKAPAAASRGSRVPLAGMALPVVSPKTVQIDDGGKVSTVHLAAPNVAGLLEAAGVPLEQNDKVVPAASSPVVDGMQIQVTRMRIEKVTERVPLAPGNERIEDPTMNMSRQVVEDPGAPGLQDVTFAIAKVNGVETGRLPVANQVIEPARNGVLRVGAKPGTEVPPVSNGHTWDALAQCEAGGNWAINTGNGFYGGVQFDQNTWERNGGLRYAPRADLATREEQIAIATVTQSRQGWGAWPVCSGRIGAR

Gene
rpfB
Protein
Resuscitation-promoting factor RpfB
Organism
Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman)
Length
362 amino acids
Function
Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity (By similarity). Plays a role in reactivating bacteria from chronic tuberculosis (TB) in mice.
Similarity
Belongs to the transglycosylase family. Rpf subfamily.
Mass
38.078 kDa
Sequence
MLRLVVGALLLVLAFAGGYAVAACKTVTLTVDGTAMRVTTMKSRVIDIVEENGFSVDDRDDLYPAAGVQVHDADTIVLRRSRPLQISLDGHDAKQVWTTASTVDEALAQLAMTDTAPAAASRASRVPLSGMALPVVSAKTVQLNDGGLVRTVHLPAPNVAGLLSAAGVPLLQSDHVVPAATAPIVEGMQIQVTRNRIKKVTERLPLPPNARRVEDPEMNMSREVVEDPGVPGTQDVTFAVAEVNGVETGRLPVANVVVTPAHEAVVRVGTKPGTEVPPVIDGSIWDAIAGCEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLATREEQIAVAEVTRLRQGWGAWPVCAARAGAR

Gene
rpfB
Protein
Resuscitation-promoting factor RpfB
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Length
362 amino acids
Function
Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity (By similarity).
Similarity
Belongs to the transglycosylase family. Rpf subfamily.
Mass
38.078 kDa
Sequence
MLRLVVGALLLVLAFAGGYAVAACKTVTLTVDGTAMRVTTMKSRVIDIVEENGFSVDDRDDLYPAAGVQVHDADTIVLRRSRPLQISLDGHDAKQVWTTASTVDEALAQLAMTDTAPAAASRASRVPLSGMALPVVSAKTVQLNDGGLVRTVHLPAPNVAGLLSAAGVPLLQSDHVVPAATAPIVEGMQIQVTRNRIKKVTERLPLPPNARRVEDPEMNMSREVVEDPGVPGTQDVTFAVAEVNGVETGRLPVANVVVTPAHEAVVRVGTKPGTEVPPVIDGSIWDAIAGCEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLATREEQIAVAEVTRLRQGWGAWPVCAARAGAR

Gene
rpfB
Protein
Resuscitation-promoting factor RpfB
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Length
362 amino acids
Function
Reduces lag phase and enhances the growth of quiescent (1 month-old culture) M.tuberculosis; works best between 8 and 128 pM. Increases the number of bacteria that can be recovered from a 3 month-old culture. Stimulates growth of stationary phase M.bovis (a slowly-growing Mycobacterium) as well as M.smegmatis cells (a fast grower). Binds N,N',N''-triacetylchitotriose (tri-NAG). A fragment (residues 194-362) hydrolyzes an artificial lysozyme substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (MUF tri-NAG). By itself has little activity on cell wall, in combination with RipA is active against cell wall extracts from a number of Actinobacteria; this activity is inhibited by PBP1A (ponA1). Sequential gene disruption indicates RpfB and RpfE are higher than RpfD and RpfC in functional hierarchy.
Similarity
Belongs to the transglycosylase family. Rpf subfamily.
Mass
38.078 kDa
Sequence
MLRLVVGALLLVLAFAGGYAVAACKTVTLTVDGTAMRVTTMKSRVIDIVEENGFSVDDRDDLYPAAGVQVHDADTIVLRRSRPLQISLDGHDAKQVWTTASTVDEALAQLAMTDTAPAAASRASRVPLSGMALPVVSAKTVQLNDGGLVRTVHLPAPNVAGLLSAAGVPLLQSDHVVPAATAPIVEGMQIQVTRNRIKKVTERLPLPPNARRVEDPEMNMSREVVEDPGVPGTQDVTFAVAEVNGVETGRLPVANVVVTPAHEAVVRVGTKPGTEVPPVIDGSIWDAIAGCEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLATREEQIAVAEVTRLRQGWGAWPVCAARAGAR