Protein
E3 ubiquitin-protein ligase RNF12-A
Function
Acts as an E3 ubiquitin-protein ligase specific for ldb1, mediating ubiquitination and proteasome-dependent degradation of excess ldb1 in a RING-dependent manner. Does not degrade ldb1 bound to lhx1/lim1, nor lim1 itself and thus contributes to the establishment of proper ldb1-lhx1/lim1 stoichiometry and the formation of a ldb1-lhx1/lim1 complex. Interferes with Spemann organizer function and suppresses secondary axis formation induced by ldb1 and lhx1/lim1.
Similarity
Belongs to the RNF12 family.
Sequence
MESADSTGKGSIEQSESQRQSQMDRLDREEAFYQFVNNLNEDDYRLMRDNNLLGTPGEITKEELLRRLQQIKEGPPQPSTEETRGDSVSTGGDPAEDSSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRTSGNPSMPSLDQSAEMPGAEDMEVSSQGEAENEPEPIPIATRSAPAEVTVEEAPIQRGQRRARSRSPDQRRTRARTDRSRSPLHHAVDPPIRRAPHSSSQTVDTSNTEEAEGSSRTRHHVSSQVQNSSSSNETEGSSRTRQHIPARQQVLGTEGQSQSTVHLSNPETRSSSQTPQTDSSTNAETTGTGQRPPTIVLDLQVRRVRPGDYRQRDSIANRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYNDNDTDPNNPTAVSPTAAVPGEAQNNANAEVRAPSAEPTEPVAPVETDEGSNVSTTATRREGRNSRGAVTFEESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLSTRNFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHIHCIDRWLSENSTCPICRRAVLVASNRESIV