Protein
Ribosomal protein S6--L-glutamate ligase
Organism
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Function
Is an L-glutamate ligase that catalyzes the ATP-dependent post-translational addition of glutamate residues to the C-terminus of ribosomal protein S6 (RpsF). Is also able to catalyze the synthesis of poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected glutamate as substrate. The number of glutamate residues added to either RpsF or to poly-alpha-glutamate changes with pH.
Similarity
Belongs to the RimK family.
Sequence
MKIAILSRDGTLYSCKRLREAAIQRGHLVEVLDPLSCYMNINPAASSIHYKGRKLPHFDAVIPRIGSAITFYGTAALRQFEMLGSYPLNESVAIARARDKLRSMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAAESVIDAFRGLNAHILVQEYIKEAKGRDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGGAASIACITEREREIALKAARTMALDVAGVDILRAERGPLVMEVNASPGLEGIEKTTGVDIAGKMIRWIERHATPEFCLKTGG