Protein
NEDD8-activating enzyme E1 catalytic subunit
Organism
Caenorhabditis elegans
Function
Catalytic subunit of the dimeric rfl-1 (uba-3)-ula-1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uba-3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of ubc-12 (By similarity). Required for cytokinesis and mitotic spindle orientation during early embryogenesis (PubMed:11847342, PubMed:19528325).
Similarity
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
Sequence
MVSVDPLATERWRSIRRLTDRDSAYKVPWFVPGPENFEALQNTKILVIGAGGLGCELLKNLALSGFRTIEVIDMDTIDVSNLNRQFLFRESDVGKSKAEVAAAFVQQRVVGCQVTAHNCRIEDKGQEFYRKFSIIICGLDSIPARRWINGMLCDLVLEMADGKPDENTIIPMIDGGTEGFKGNARVIYPKFTACIDCTLDLYPPQVNFPLCTIAHTPRLPEHCIEYIKVVVWPEEKPFEGVSLDADDPIHVEWVLERASLRAEKYNIRGVDRRLTSGVLKRIIPAVASTNAVIAASCALEALKLATNIAKPIDNYLNFTQIHGAYTSVVSMMKDDNCLTCSGGRLPFEVSPSSTLESLIIRLSERFHLKHPTLATSTRKLYCISSFMPQFEQESKENLHTSMKDLVSDGEEILVSDEALSRALTLRIQLI