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recD

Gene
recD
Protein
RecBCD enzyme subunit RecD
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Length
640 amino acids
Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Similarity
Belongs to the RecD family.
Mass
72.864 kDa
Sequence
MLSVLHKLKELRILSQGDYYFAKLIADKQCHTDYAEPVKNLAILLAALCSWRYTQGNTCSQLDRYLEHNLFGLAYRTTEEDYLAEIHEKIGYLPVEDWQNALCGHMAFTQDPVNQIAPMAFQFGALYFYRAWQDEYRIVQYIKNTLKKYRTLAFSYDEIHQKLEKYFPEKQEKTDWQKVAVATAIKSPFSIITGGPGTGKTTTVTRLLLVLQELFDCKLHIKLVAPTGKAASRLEESIKNALGFMQEKMNVSHSLFNAIPQKASTLHSLLGVNAFNDYTRYNSHNPLQLDVLVVDETSMIDLPMMAKLINALKPETRLILLGDQAQLASVEAGAVLGELAQFVTQPYSHEQAAYLLATTGYKVEGSDCSNPIRDCLCHLTESRRFDKDSGIGKLSEFIQKGKADDSLELFDHYPQELHFNSLNDEGDAVNQVVKSAVENYRTFLKMLDDLRKQKIDPNAKNEQGISYAEAIQVQFNSVRFLTALRNNNLGVENLNKEIALALREQKLLWFRNEQDWYIGKPIMITENDHNVRLYNGDIGLCLANGKVWFGNREVLTNRIPAHEPAFMMTIHKSQGSEFKHTVMVLPTEVNPVLSRELVFTGVTRAKKELTVFADEKIWKTAIRQTVKRQSGLGKLLEDLN

Gene
recD
Protein
RecBCD enzyme subunit RecD
Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Length
618 amino acids
Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Similarity
Belongs to the RecD family.
Mass
71.577 kDa
Sequence
MTITSILQKMVKLKIITSSNFYLSTMLFNKNTLATFLSICVNHFTNHGHVCLPHVLIQHQKIFFTNHIALITKLWEIAKINTCGWIEQLLNSNTISNGSKNTPFIFYKNNFYINKFWLAEQKILKFISQKQNFKLINKNKIQKILKNIFNTNIDDTQKLAIILSIINNITFIIGGPGTGKTTIILKILLVLIRLHKKKINIKLTALTGKAANRLSESIHENIKKLNLTEHEKNIFNQKTTTLHNLLDLKPNKSFNYSCCKNKLYNLNLLIIDEASMIDITIMDKLISSIPKTTKIIFLGDYNQLPSISGGNILKNICSYYQEGFSTTTNKFLKQLFPNRITIKTIKKHQFSNINDKIIMLKKSYRFKKKSDIFKISEMLTKNKINNIKTLYQNINNDVNFKQISSKTNYNNIIFEITNSYSKYWSALKQSLPFLKIINIFNNYKILCILKNGLFGITELNLAIEKEMKKIGWIKKVTIINGQTWYFGKPILILKNNEEMNLFNGECGLTLLDANKKLKVFFLPKNQEQIYSIPIHLVPEHQTNWTMTVHKSQGSEFSEVVLILPTIMTSILTKELIYTAVTRSKKKLTIYSDENIFIKSLKKNIIRYSGLSIHQKIKF

Gene
recD
Protein
RecBCD enzyme subunit RecD
Organism
Escherichia coli (strain K12)
Length
608 amino acids
Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid (>1 kb/second) and highly processive (>30 kb) ATP-dependent bidirectional helicase. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator, 5'-GCTGGTGG-3') sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to Chi site, by nicking one strand or switching the strand degraded (depending on the reaction conditions). The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang which facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. In the holoenzyme this subunit contributes ssDNA-dependent ATPase and fast 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Similarity
Belongs to the RecD family.
Mass
66.902 kDa
Sequence
MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSHDAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMILCGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAVALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGKALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDLPMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTGTHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTDIEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFGVAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGTRVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE

Gene
recD
Protein
RecBCD enzyme subunit RecD
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)
Length
602 amino acids
Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Similarity
Belongs to the RecD family.
Mass
69.495 kDa
Sequence
MLILLKKAVKLKIIRPIDFYFSQFIAQKNNIVMLVAACVSYESSRGYISLPIKYFEKHYFFSSSNEVFIKKILTLLEKKINWPVELLKHASIGNGGTSTPLVLHKKKIYLYKMWKSESNIFNYLYTKNKKNKINQKKCSKILENLFPQKNMSFQKIAVALTLINNITFIIGGPGTGKTTTILKIIIALIKSSKKSIKIQLSAPTGKATTHLNEILKNNIFDLYFSEKEKCSLPSTATTIHQLLGIQKISQKSFFNKSNCLDLDVLIIDEISMVDILMMEKILSSISKNTKLIFIGDHNQLGPIESGSILRKICYYANDGYSFKSMISIEKLTQYKLCKKINKKTTNFISDNICVLNKNYRFNKNSGIYTLSNAIFKKKTRIIESLFDNSIKNIFFYETNSSEQYKKMIKNICLNYEDFWEKIYKKATMKEIIESFQNYQVLCILHDGLFGVNIINKKLEENMYKKNIIKYFYIDGEEWYIGKPIMIINNNRALNVSNGNIGITNINKNGILQVSFLKENNTINNIPVKILRNYKTAWAITVHKSQGSEFMNTALILPNFNSHILNKDTLYTGITRSRKILSIFSDKKIFLNTIFKNTNKILF

Gene
recD
Protein
RecBCD enzyme subunit RecD
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Length
602 amino acids
Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Similarity
Belongs to the RecD family.
Mass
70.408 kDa
Sequence
MNMSHLLQNFANKKIITLVDFYFSQFISKKNSIIMLISACVSFESKNGHIFLPIEYFEKNCFFSISNKQFINKILKCLNKKKINWSLELSEHISCGDGSIITPLVFYKDKIYLYKIWKAEKKILERLYEKNQFDTIDTQQCLNILNNLFSKKKHDLQKIAVILTLINNIIFITGGPGTGKTTIILKIIIALIKNAKKKIKIQLSAPTGKATENLIEILNDKWLNRYLLKEEKKQFSFNPIMTIHQLLGISKKSEKIFFNKNNLLMIDILIIDEASMIDILMMSNILSALSKKTKIIFIGDHNQLKPVKSSSILKYICSYAEDGYSLKTQSILQEITQNSIINYKINKKNTSYISDKICVLKKSYRFEKKTGIYILSNAVYNKETKIFEKLFKNSIENVFFYEINSEIEYKKMINIIINNNKIFWRKIYEKKNIKEIIKTFKNHQILCIVKNGFFGVNFINKILEEEMYKRNIFNKRFYINNKLWYIGKPIIITENNQCLGIFNGEIGITNLSQKNTLQVSFLKKDNFIENIPIEILKNYKTSWSITVHKAQGSEFNNISLILPNKNLKILKKDILYTGITRTRKQLNIFSTKEIFIKTVLKN

Gene
recD
Protein
RecBCD enzyme subunit RecD
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Length
575 amino acids
Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Similarity
Belongs to the RecD family.
Mass
61.747 kDa
Sequence
MKLTDVDFAVEASGMVRAFNQAGVLDVSDVHVAQRLCALAGESDERVALAVAVAVRALRAGSVCVDLLSIARVAGHDDLPWPDPADWLAAVRASPLLADPPVLHLYDDRLLYLDRYWREEEQVCADLLALLTSRRPAGVPDLRRLFPTGFDEQRRAAEIALSQGVTVLTGGPGTGKTTTVARLLALVAEQAELAGEPRPRIALAAPTGKAAARLAEAVRREMAKLDATDRARLGDLHAVTLHRLLGAKPGARFRQDRQNRLPHNVIVVDETSMVSLTLMARLAEAVRPGARLILVGDADQLASVEAGAVLADLVDGFSVRDDALVAQLRTSHRFGKVIGTLAEAIRAGDGDAVLGLLRSGEERIEFVDDEDPAPRLRAVLVPHALRLREAALLGASDVALATLDEHRLLCAHRDGPTGVLHWNRRVQAWLAEETGQPPWTPWYAGRPLLVTANDYGLRVYNGDTGVVLAGPTGLRAVISGASGPLDVATGRLGDVETMHAMTIHKSQGSQVDEVTVLMPQEDSRLLTRELLYTAVTRAKRKVRVVGSEASVRAAIARRAVRASGLRMRLQSTGCG

Gene
recD
Protein
RecBCD enzyme subunit RecD
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Length
575 amino acids
Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Similarity
Belongs to the RecD family.
Mass
61.747 kDa
Sequence
MKLTDVDFAVEASGMVRAFNQAGVLDVSDVHVAQRLCALAGESDERVALAVAVAVRALRAGSVCVDLLSIARVAGHDDLPWPDPADWLAAVRASPLLADPPVLHLYDDRLLYLDRYWREEEQVCADLLALLTSRRPAGVPDLRRLFPTGFDEQRRAAEIALSQGVTVLTGGPGTGKTTTVARLLALVAEQAELAGEPRPRIALAAPTGKAAARLAEAVRREMAKLDATDRARLGDLHAVTLHRLLGAKPGARFRQDRQNRLPHNVIVVDETSMVSLTLMARLAEAVRPGARLILVGDADQLASVEAGAVLADLVDGFSVRDDALVAQLRTSHRFGKVIGTLAEAIRAGDGDAVLGLLRSGEERIEFVDDEDPAPRLRAVLVPHALRLREAALLGASDVALATLDEHRLLCAHRDGPTGVLHWNRRVQAWLAEETGQPPWTPWYAGRPLLVTANDYGLRVYNGDTGVVLAGPTGLRAVISGASGPLDVATGRLGDVETMHAMTIHKSQGSQVDEVTVLMPQEDSRLLTRELLYTAVTRAKRKVRVVGSEASVRAAIARRAVRASGLRMRLQSTGCG

Gene
recD
Protein
RecBCD enzyme subunit RecD
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Length
554 amino acids
Function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity (By similarity). Unlike the case in E.coli, suppresses RecA-dependent homologous recombination, is instead required for single-strand annealing pathway repair of DSB.
Similarity
Belongs to the RecD family.
Mass
59.516 kDa
Sequence
MSLLEAFAEILEPADVHVAQRLTVLADEPDASVELAVALAVRALRGGSVCVDLRSVAGQVELPDLPWPDPDAWLAALAASPLLGQPPVLRLFGDLLYLDRYWLEEQQVCDDVLALVSARPGGAVPDVSRLFGAGFEEQRAAAKVALSQGLTVLTGGPGTGKTTTVARLLALLAEQAALAGKPSPRIALAAPTGKAAARLQEAVQLEIDQLDLIERRRLTGLHATTLHRLLGPRPDTSSRFRHHRANRLPHDVIVVDETSMVSLTMMARLLEAVRPQTRLVLVGDPDQLASVEAGAVLADLVEGLGSRGVAELKTSHRFGESIGALASAIRTGDADAALAVLAAGGEHIEWVHEDPVEQLREVVVPHAMRLRQAAILGDQREALATLDEHRLLCAHRRGPAGVDHWNRQVQRWLGEETGEPLWGSWYVGRPILVTANDYGLKLYNGDTGVVVATPDGMRAVIGDAGTFATGRLTDVETMHAMTIHKSQGSQADEVTVLLPSEDSRLLTRELFYTAVTRAKTRVRVVGAEAEVRAAIERQAVRATGLRKRLRPAGV