puf

Ubiquitin carboxyl-terminal hydrolase puf

3912 amino acids

Ubiquitin hydrolase that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed:24173801). Essential for Myc-mediated cell growth and proliferation in developing eyes and wings (PubMed:24173801). In the wing and eye, the deubiquitinating activity acts as an antagonist to the SCF E3 ubiquitin-protein ligase member archipelago (ago) to regulate Myc and CycE stability and thus control cell growth and proliferation (PubMed:24173801). Also appears to regulate ago by modulating its induction by Myc (PubMed:24173801). May also promote cell apoptosis in the wing imaginal disk, acting in an apoptotic pathway that appears to be largely independent of Myc (PubMed:24173801). Required for preventing the activation of the immune deficiency (Imd) and Toll signaling cascades under unchallenged conditions (PubMed:25027767). Also appears to be involved in modulating the differential expression of certain antimicrobial peptides (AMP) in response to infection by either Gram-positive or Gram-negative bacteria (PubMed:25027767).

Belongs to the peptidase C19 family.

440.375 kDa

MCEVCADFQNLLELYEVRVASSDLKFQLLLKSEIETTFNYIQSWPQRQCMCLYRDTKNYDRFNLVVQSLICLTVQHLKHIDHLIDNYKRLTASAAQVVAQAQQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTANPQHKSHLQYTEEPWILPEVEKLLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQAEEAHHLALICDLHDNDLPIYLLRNVSLFCNSGGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKLWLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVAVNFDTEGLALAFKYFTSTTLTMRLAGMAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVEIVKQAHVLLNFLAVENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDLLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFASFQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALWEWLAHDPECADCYFSWLQAQAKGGDQHALGIEALQHLYLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISASASAVGMYHLWKIALRAQSNDVSLAAIQYINMYYMGQQLRLEKEFVSQCMENLVQAATALESIDDENALMRVQRGLLLLNTHLDTFRRRYAFHLRRWAIEGKGIGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSLGGRGARRKESNLEHPSMLPPPPKECLPTVLLLQPKYFEKLFCLMQTLGDMQPQASTVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLATKRKQIKQKFRDLLDPNNLQKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQHQASSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDDGLVGLLNLMSNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGPKQPYPWDYWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQKDMAEFFIDLVSKLEDMTPDLKHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFDPSQIAAECFGGEMTSKTYDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMAAKLSVSFFIETFIHAKEKPTMVPWVELLTKQFNASQEACEWFLSHMSQEPYWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFSAIAGGKGFPFLYQQIKDGINPHQTKHLIHALCRWDERLATQIIGMLFASVTKHTELCAPFFKLLTLLTETQGGPVGLPCFTQLILPRMWDAAEYCPQSVLDWLSLQATKNKIAHAWILQSAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLALNPHSYRDINSDAQAVLHQVITLLLRLLRPARVYADIGAHGTTKLTAYFNLLSYCMVSKTEKLMASSYMRSLWELFHPRLSEPSVPAHHNKHALLTFWHHSLVDCPENAAQVANCPEITRNIAFNYILADHDDAEIVTYNRSMLPAYYGLLRLCCEQSRALTRQLSQHQNLQWAFKNITPHPTQYAAAVDELFKLMALFATRHPDASEQEKLDVTQFRRAVIVSYTSSLDARVSWSTLISALKILVDNEEDRTMVIFNGGIEMCFEALHTLHSMHHEATACHVAGDLFDLLGEMLLLLATLRTRTDSPAQKKQQQQQQQLEQQLQLQQQQMLQKQQQQSQSQTQTPQSPQQKEKQLQQQMQQHLQLQQLQQMQFQQQHFLRQQHQHSALAKALPDAVKRLATLLNTFNSPEISRMALEVLKELVRNPSLETISILAPILINCHLSVANAPNAIGPLGPYFPRRGAKHTPWPLGAKNSPRPPRPMVQMCIALAELTPRGLDADYDVQVESFYRPYHDFIDVMMRMCVNTGTLNDTLVKLQCLVAIESTPLHFTYFPKFWVGIHNNALTHKYVELLVKNQLLVEYLHNVLRDERSMLKDACVREFLELYYHKVAAQLPVARMIYTINYGMHSKDDIDELCGDLFAIRIIAQATGVPASVRKELRGSLRALQNKSERFRKESERDPFPNKKQKRDSQKIKEKEHPQPESEKETSTENDKPSDVSMESSGNAEQATDSTKPPTPAGSDDEQMDKTSVPSSDDETELEDELQPTPKRNKKASNKKTAQDRVNEEREKRLITLITMESYIQSIFAILKRDASVPSSGATKEPSEEPCGEASTSAAAAVKLSRPKGACTPPEPITDVNDTRCNIDTETEAEADEQSPKTSQTNGSQQNESPPAATSADTAPANPSPAPAAAVASTSQAASPTQI