psiM

Protein psiM

733 amino acids

Belongs to the prespore-cell-inducing factor family.

79.232 kDa

MKKINNNKIFVLFLTILLYLLNITTAQKPVSINIKIFDQLPLYNDNFGSQSRAATIKSMVATSLNTTFKTPSLGSATSSLPSPQLFQYWFQSNFANETKNSGLNAPLTRTITLNYDSDSGNYIFNNPNFFLIDTDGFDKTASNRIYKDSTGTYHNYHYCVAFNYVFKPDGQGKEMFKFTGNDDVWVFVNNKLLLDGGGVRSPEATTVDFTKAGLVKSQETPLDFFYCERSTNSPPSFKIETNLGGLYCKAYDYCGVCNGDGGTCCNPQTDCLTQLENGTLINDKCTIPICPPLDTQIDKNLGIGFFCQHSQIPDPYAGSLCEKGECDSLTGIWTRNTSICPTDKDLAPQCRKQATCDPKLGCQSTYLCNNVCDIGTCNNGTCTQKTSNDCVQELDRGVEDKCFTYKCVPNVGCSKTPICPRGTDPCKNYTCSGGICGTVDIPQSECSCPCPGLNKCNTRICNPDNTCTLFNLTEINDGNPCTDDLCDPVTGDITHVMTTRCSGCNTCDVKTGKCVAQDSHCDDGNPCTDNACVASANSTANSQVGQCTQTPTAKCDLGDKCMVYSCTLEGGCNATQRVCPNSGACKIGYCEPGFGCKLKDRVCSSDWYCIEAQCDAKYGCIQFDRRCAPDNSKCQEGVCVNNTAIGKNDGKCISEDYDPKPFVCQKAALVSTAVIASVVVVGAVVLGAAIFAGKKGYDAWKTSQGNVMAASQANPLYTQSSNGGENPLYNSPT

psiM

Psilocybin synthase

309 amino acids

N-methyltransferase; part of the gene cluster that mediates the biosynthesis of psilocybin, a psychotropic tryptamine-derived natural product (PubMed:28763571). The first step in the pathway is the decarboxylation of L-tryptophan to tryptamine by the decarboxylase psiD (PubMed:28763571). 4-hydroxy-L-tryptophan is accepted as substrate by psiD as well (PubMed:28763571). The cytochrome P450 monooxygenase psiH then converts tryptamine to 4-hydroxytryptamine (PubMed:28763571). The kinase psiK catalyzes the 4-O-phosphorylation step by converting 4-hydroxytryptamine into norbaeocystin (PubMed:28763571). The methyltransferase psiM then catalyzes iterative methyl transfer to the amino group of norbaeocystin to yield psilocybin via a monomethylated intermediate, baeocystin (PubMed:28763571). PsiK kinase can also turn psilocin into psilocybin (PubMed:28763571). This activity may represent a protective mechanism to rephosphorylate the unstable psilocin to the stable psilocybin in case of intracellular ester cleavage (PubMed:28763571).

Belongs to the methyltransferase superfamily. METTL16/RlmF family.

34.434 kDa

MHIRNPYRTPIDYQALSEAFPPLKPFVSVNADGTSSVDLTIPEAQRAFTAALLHRDFGLTMTIPEDRLCPTVPNRLNYVLWIEDIFNYTNKTLGLSDDRPIKGVDIGTGASAIYPMLACARFKAWSMVGTEVERKCIDTARLNVVANNLQDRLSILETSIDGPILVPIFEATEEYEYEFTMCNPPFYDGAADMQTSDAAKGFGFGVGAPHSGTVIEMSTEGGESAFVAQMVRESLKLRTRCRWYTSNLGKLKSLKEIVGLLKELEISNYAINEYVQGSTRRYAVAWSFTDIQLPEELSRPSNPELSSLF

psiM

Psilocybin synthase

309 amino acids

Methyltransferase; part of the gene cluster that mediates the biosynthesis of psilocybin, a psychotropic tryptamine-derived natural product (PubMed:28763571, PubMed:30283667). The first step in the pathway is the decarboxylation of L-tryptophan to tryptamine by the decarboxylase psiD. PsiD does not decarboxylate phenylalanine, tyrosine, or 5-hydroxy- L -tryptophan (5-HTP) (PubMed:30283667). 4-hydroxy-L-tryptophan is accepted as substrate by psiD as well. The cytochrome P450 monooxygenase psiH then converts tryptamine to 4-hydroxytryptamine. The kinase psiK catalyzes the 4-O-phosphorylation step by converting 4-hydroxytryptamine into norbaeocystin. The methyltransferase psiM then catalyzes iterative methyl transfer to the amino group of norbaeocystin to yield psilocybin via a monomethylated intermediate, baeocystin. PsiK kinase can also turn psilocin into psilocybin. This activity may represent a protective mechanism to rephosphorylate the unstable psilocin to the stable psilocybin in case of intracellular ester cleavage (By similarity).

Belongs to the methyltransferase superfamily. METTL16/RlmF family.

34.312 kDa

MHIRNPYRDGVDYQALAEAFPALKPHVTVNSDNTTSIDFAVPEAQRLYTAALLHRDFGLTITLPEDRLCPTVPNRLNYVLWVEDILKVTSDALGLPDNRQVKGIDIGTGASAIYPMLACSRFKTWSMVATEVDQKCIDTARLNVIANNLQERLAIIATSVDGPILVPLLQANSDFEYDFTMCNPPFYDGASDMQTSDAAKGFGFGVNAPHTGTVLEMATEGGESAFVAQMVRESLNLQTRCRWFTSNLGKLKSLYEIVGLLREHQISNYAINEYVQGATRRYAIAWSFIDVRLPDHLSRPSNPDLSSLF