PRPF8

Pre-mRNA-processing-splicing factor 8

2335 amino acids

Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome (PubMed:10411133, PubMed:11971955, PubMed:28502770, PubMed:28781166, PubMed:28076346, PubMed:29361316, PubMed:30315277, PubMed:29360106, PubMed:29301961, PubMed:30728453, PubMed:30705154). Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

273.6 kDa

MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Prpf8

Pre-mRNA-processing-splicing factor 8

2335 amino acids

Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

273.616 kDa

MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

prpf8

Pre-mRNA-processing-splicing factor 8 homolog

2327 amino acids

Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

272.196 kDa

MDDTNSNINQSNESQHLEEKAKKWIQLNNKKYSEKRKFGAVEIRKEDMPPEHLRKIIKDHGDMSNRRFRDDKRVYLGALKYMPHAILKLLENIPMPWEQVKYVKVLYHLSGAITFVNEIPFVIEPIYIAQWATMWVTMRREKRDRTHFRRMKFPLFDDEEPPLDYSDNILDNEVEDPIQMELDENDDSEVIDWLYDSKPLVNTKFVNGSSYRKWRLNLPIMSTLFRLASPLLSDLTDSNYFYLFDDNSFFTSKALNMAIPGGPKFEPLFRDVDDDDEDWNEFNDINKVIIRNKIRTEYKIAFPYLYNSRPRKVKTPTYHTPNNCYIKNDSPDLPGFYFGAALNPIPSYKTSGNKNEQSEYGTEDDEFQLPEEIETILSKTEIEHDNLANGIQLYWAPRPFSLRSGTTRRAEDIPLVKSWYKEHCPSEHPVKVRVSYQKLLKCHVLNKLHHRKPKAQTKRNLFKSLKATKFFQSTEIDWVEAGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFYLKPIKTLTTKERKKSRFGNAFHLCREILRLTKLVVDVHVKFRLGDADAFQLADAIQYLFSHLGLLTGMYKYKYRLMRQIRMCKDLKHLIYYRFNTGAVGKGPGCGFWAPMWRVWLFFLRGIVPLLERWLGNLLARQFEGRQTKGMAKTVTKQRVESHFDYELRAAVMHDILDMMPEGIKANKSRIILQHLSEAWRCWKSNIPWKVPGLPIPIENMILRYVKSKADWWTNIAHYNRERIKRGATIDKTASKKNLGRLTRLWLKAEQERQHNYLKDGPYVSAEEAVAIYTTTVHWLEKRRFSAIPFPQTSYKHDIKILTLALERLKEAYSVKSRLNQSQREELSLVEQAYDNPHDALARIKRHLLTQRTFKEVGIEFMDMYTHLVPIYDVDPFEKITDAYLDQYLWYEADKRQLFPNWVKPSDNEPPPVLIHKWCQGINNLDQVWETSQGECVVLLETQFSKVYEKMDLTLMNRLLRLIVDQNIADYMSGKNNVVINYKDMNHTNSYGLIRGLQFASFIFQYYGLVLDLLVLGLERASALAGPPNLPNSFLTFPSVQTETAHPIRLYSRYVDRIHVLYKFTADEARKLIQKYMSEHPDPNNENVVGYNNKKCWPRDCRMRLMKHDVNLGRAVFWQIKNRLPRSLTTIDWEDSFVSVYSKDNPNLLMNMAGFDIRILPKCRTPLDQLAPKDAVWSLQNVNTKERTAQAFLRVDTESQERFENRIRMILMASGSTTFTKIVNKWNTALIGLMTYYREAVVTTREMLDILVRCENKIQTRVKIGLNSKMPNRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLKYSKQTDTGITHFTSGMSHDEDQLIPNLYRYIQPWEQEIKDSQRVWAEYAIKYEEAKSQNKNLTLEDLEDSWDRGIPRINTLFQKSRHTLAYDKGWRVRTDWKQYQVLKNNPFWWTNQRHDGKLWNLNNYRTDIIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTHAQRSGLNQIPNRRFTLWWSPTINRKNVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESLVMDLCQVFDQELDNLEISVVNKEAIHPRKSYKMNSSCADILLRATHKWQVSRPSLLNDNRDTYDNTTTQYWLDVQLKWGDFDSHDIERYSRAKFLDYTTDSMSLYPSPTGCLIGLDLAYNIYSSFGNWFLGVKPLVQKAMAKILKSNPALYVLRERIRKGLQLYSSEPTEPYLSSQNFGELFSNKIMWFVDDSNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTDVWLGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIATRKGMMDPLEVHLLDFPNIVIQGSELQLPFQACLKVEKFGDLILKATEPKMVLFNIYDDWLSTIHSYTAFLRLILILRALHVNLERTKIILKPNKNVITQPHHIWPTLTEQEWLTVEGSLKDLILADFGKRNNVNVASLTQSEIRDIILGMEISAPSQQREDQIAEIEKQKTEASHLTAVTVRSTNIHGEEIITTATSPHEQKVFSSKTDWRVRAISATNLHLRTNQIYVNSDNAKETGGFTYVFPKNILKKFITIADLRTQIMGYCYGISPPDNPSVKEIRCIVMPPQWGTPVHVTVPNQLPEHEYLKDLEPLGWIHTQPTELPQLSPQDVITHSKIMSDNKSWDGEKTVIISVSVAWPCTLTAYHLTPSGFEWGKNNKDSLNYQGYQPQFYEKVQMLLSDRFLGFYMVPDRGSWNYNFMGVKHSTNMTYGLKLDYPKNFYDESHRPAHFQNWTQMAPSANDDEENQPENENLFE