Protein
Nonribosomal peptide synthetase pngA
Organism
Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Function
Nonribosomal peptide synthetase that mediates the biosynthesis of phenguignardic acid (PubMed:26851300). PngA alone is sufficient for phenguignardic acid synthesis (PubMed:26851300). PngA first activates phenylpyruvic acid (PPA) through its A domain to AMP-PPA (PubMed:26851300). The PPA unit is then loaded to the T domain and eventually transferred to the TE domain (PubMed:26851300). Another PPA unit is then loaded onto the T domain (PubMed:26851300). The TE domain likely promotes the enolate formation on the attached unit, followed by a nucleophilic attack on the carbonyl to yield an ether linkage between the two units (PubMed:26851300). Finally, the TE domain probably catalyzes a similar reaction to give the cyclized dioxolanone core and releases phenguignardic acid (PubMed:26851300).
Similarity
Belongs to the NRP synthase family.
Sequence
MNKKLKLFSMPGAQTSQIVIMLFQSLLHLLEAIASREPTRYIITYSIGNTHTPEIFSYSDLLQSARKAAGALRFKYHVVPGSVVLLHFNDHWNSMLWFWATLIADCIPAMSTPFSNNPETRLRHLKHLSTTLRSPKCLTTASLAAEFAGQEYITPICVQSLDYENLVHLPIKEGGDIAVLMFTSGSSGHCKVVPLTHEQILASLSGKAWTFPLPDNTAQLNWVGMNHVASLVEVHLFSIYTHSDQVHIPTVEVLSHVTLFLDLIHRHRVSRTFAPNFFLAKLRAALSADDTLAKYTGSLSNLRYIVSGGEANVTQTINDLAQMLKKCGAVSNVIVPAFGMTETCAGAIYNTSFPQYDVEHGLPFASVGSCMPGIQVRIVQLNGNGNSVPPGTVGNLEICGPVVLKGYFNNPAATKSTFTNDNWFKTGDLAFVDDNGMLVLAGREKDSIIVNGANYSPHDIESAIDEANIPGLISGFTCCFSTFPPSADTEEVIIVYLPNYTPADTVRRSETAAAIRKVAMMSVGVRATVLPLDRTMLEKSTLGKLARGKIKAAYERGDYKSYQEANEQMMALHHKVSHHQPRSGLEQSLLGVFTRTIPENLTEDFDVLTSIFDLGITSIELLKLKRGIEDLIGHGQIPLITLMTNPTIRTLSDALKQHAQQRDCSIYNPVVVLQSQGKKPPIWLVHPVGGEVMIFMNLAKFIIDRPVYGLRARGFNDGEDPFHTFEEIVSTYHASIKEKQPSGPYAIAGYSYGAKVAFDIAKALEHNGDEVRFLGLLDLPPSLNGTQMRAVAWKEMLLHICRMVGVIREEGIKKIYPRLEPENISPRHAIETVMGEADVTRLAELGLTASALERWANLTHALQRCIVDHKTNGSVAGADAFYCDPMASMAISNEQWACDYIGKWSDHTRSPPRFHHIAGTHYTILDAENIFSFQKTFLRALNDRGI