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pkfA

Gene
pkfA
Protein
Non-reducing polyketide synthase pkfA
Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)
Length
2193 amino acids
Function
Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of aspernidine A, a prenylated isoindolinone (PubMed:23706169). The starting point of the biosynthesis of aspernidin A is the production of orsellinaldehyde by the non-reducing polyketide synthase pkfA, via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units (PubMed:22510154). Hydroxylation, methylation of one of the phenol groups, and prenylation, presumably catalyzed by the prenyltransferase pkfE, would be needed to yield aspernidine D (Probable). Subsequently, the cytochrome P450 monooxygenase pkfB is responsible for hydroxylation of aspernidine D to yield aspernidine E (PubMed:23706169). The dehydrogenase pkfF may be responsible for further oxidation of aspernidine E to form a dialdehyde intermediate which is further transformed in a series of steps, some of which are enzyme-mediated, to generate aspernidine A (Probable). The possibility that additional enzymes outside of the cluster are involved in aspernidine A biosynthesis cannot be excluded (Probable).
Mass
241.709 kDa
Sequence
MQGSSHTLLAFGPQSLSFDKRALIKLRGDISSGRYGQWIQDTIAGLPGFWTALSEALPELRELLPPQERLQDLDRLLNHSIDDIEEGLSISNMILTPLVVLTHLTEYERYLATNQHTSDRTTATGFCTGLLSTFAIASSRNDQELAKYGAVALRLAMLVGALVDAEERRQELQGNGRSVSYSIAWMSEEQQQRLQEILGQRHPETYLSVRYDRARATLTSSGRAAADLAQELREIGLTVAQVRLQGRFHDPSESRKAYTDALADLCNRTPELQFAPTPAMSLAYPKIDNASLHEVAVRSMLVQHCDWYDTFKAAYPEVSSPARVVCFGSERCIPPTLAKRTVKIESQHQASDAFASLDQENMIAVVGMSIKTAGADDVDEFAQMLRTGTSQHQAIMEDDINFNTPWRTDKGSRTWYSNLIRDRSQFDHSFFKMSPREAAAMDPQHRLFLQAAYQAVEQSGYFLEPGHNETKLPEDSVGVFHVSCYDPNAYTATGNLRAFLAGRVSHHFGWTGPALTLDTACSSSAVAIHLACRSILSGECSAALAGGVAVMSSPFWFQNLGAASFLSPTGQCKPFDEKGDGYCRSEGIGCVMLKKMSAAVANNDQILGCIGASAVHQNQNCTAVVVPNKPSLAPLFKQVISKSGLTPADISLVEAHGTGTAVGDPAEYASIADALNVASRPSPLILGSVKGHIGHTEAAAGVVSLIKVLMMMNEGFIPPQASHTRLSPRITAATDKLRISTSLRPWETDQKAALVNSYGASGSNTSMVILQLPKRHTFPHQGVTKDGLAQPFWISGTNEVEITAYCARLAEYIRTRPTVRLDDLSFNLSRQCNRTLARQLVFSCRSMSELQAMLSRGASAEGSSKVVPTERPVILCFGGQVSTFVGLNRLVYENVGLLRRYLDECDMAMTNMGLESIYPEIFSTSPVQDTVKLQCMLFAMQYSCARCWLDCGVSTRIAAVVGHSFGELTALSISGVLSVQDTIKLVAGRARLIRDQWGSDRGAMMAVEGDESTIQQLLEEASQQTPSDHPATIACYNGPRSFTLGGSTAAIEGVKETLLSKYPYIRHKRLNVTNAFHCSLVGNIVEPLNQLGEELTFNSPVIHIEHSSSTPSTGARPSSTFVADHLRKPVYFYHAIERLARAYPDSIWLEAGSNSTITTMAGRALRPTSDIVGQSHHHFQALNITHGGRGSDSLTETTLSLWKEGLRVSFWQHHPVQKDQYEHLILPPRQFARTRHGLDIIAPKTDQGAAQQTGVPKPDGLWTFVGYEDNNRSRPRFLINSNSEKYLAMVEGHVMARTAAICPAGLEVDMAIEALFSLQPEWKERRLQPMLRDMVNSAPVCLDPIRKFWLQLSAADESCMLWDWNITSTREGSSSSLLHVKGSLQIRSPEDPLYRNDFARLERLVPYRQCTDILALAGAQPDDLDDEIHVLQGRNVYQGFSDVISYTPLYRSLRRLVGRGNESAGCVIRKRSHETWLDVPLSECFMQTGGIWVSCMTDRSPDDVFIATGCELWMRSPSLADVKEKGEDGTEKWHVLARHLRVSDKVYLTDVFVFDSRTGALTEVIMGTKFSRLPKTVMRKILSDFHPGNPSVHEKDIVTAAPKQPIIQAQEIVLAEAPRKHKSVQKRDITGQVCQLLSSVSGIEPAKINQNAELSDLGVDSLMAMELAREIEGVFKFSPDHNDLLEATTVEKLTNYISSSIPGSSAASENIDNHGSPNDTDSSGESDPATPTVACSSTDDFREPFIITSSRDEAEVLSLAESDDTFLKSQRPIVEMAKQNTDREEIAEQYVARYTAGFIPPVSSRSSAREHSGNVVIITGATGSLGSHLVASFARDPSVRTVICLNRRSKTPGTLRQSQALSTRKLQLTEAESSKLRMLDGNTSEPHLGLAADEYTWLARNATHIVHNAWPMSLARPIHTFVPQFQALRNLLDLAREMACNCSPNGMRVGFQFVSSISVVGSAAQQRVLEQRVDLRSSLPIGYPEAKWVCERMLDETLHRYPDHFRPMVVRPGQITGSSHTGCWPHTEHMPLLIKTAQTLQAWPDLDGPLHWVPVDTVAQTMVDLLLPGIESDSNQTGTTLNKSEPDAYPVYHIDNPVGQPWKEMTPIITSALNIPSDRVIPYQEFLNLLRSSPLDRDREIPAAKIMDFFTRDFEHMACGGLVLDTTLSQEHSATLAAQGPVTEEENMKILTL