phsA

Thiosulfate reductase molybdopterin-containing subunit PhsA

758 amino acids

Component of the PhsABC thiosulfate reductase that catalyzes the reduction of thiosulfate to sulfite and hydrogen sulfide, with menaquinol as the sole electron donor. Proton motive force (PMF) is required to drive transmembrane electron transfer within the reductase. The PhsA subunit contains the active site molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor.

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

82.8 kDa

MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGTRICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLATAFGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFSSKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAAQADVPADVIVRVTRELAACAPHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVAPQFKYIAAGGGVVQSIIDSALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPECTYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGYLEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAGAKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA

phsA

O-aminophenol oxidase

643 amino acids

Could be involved in the spore pigmentation and melanin production. Catalyzes the oxidative coupling of 2-aminophenols to form the 2-aminophenoxazinone chromophore. 2-aminophenoxazinone synthesis proceeds via a sequence of three consecutive 2-electron aminophenol oxidations. First, the o-aminophenol is oxidized by two electrons to the quinone imine, which then conjugates to a second o-aminophenol molecule while still bound to the enzyme. This product is further oxidized by two electrons to give rise to the p-quinone imine. The last two steps of the reaction, another conjugation to generate the tricyclic structure and a final two-electron oxidation to yield the 2-aminophenoxazinone product, are thought to be non-enzymatic. It can also uuse 3-hydroxyanthranilic acid (HAA), 4-methyl-3-hydroxyanthranilic acid (MHA), 3,4-dihydroxy-L-phenylalanine (L-DOPA), ferrocyanide and thiophenol as substrates.

Belongs to the multicopper oxidase family.

70.245 kDa

MTDMIEQSDDRIDPIDGVLADGVLADDVLAKEREQAPAPGELTPFAAPLTVPPVLRPASDEVTRETEIALRPTWVRLHPQLPPTLMWGYDGQVPGPTIEVRRGQRVRIAWTNRIPKGSEYPVTSVEVPLGPPGTPAPNTEPGRGGVEPNKDVAALPAWSVTHLHGAQTGGGNDGWADNAVGFGDAQLSEYPNDHQATQWWYHDHAMNITRWNVMAGLYGTYLVRDDEEDALGLPSGDREIPLLIADRNLDTDEDGRLNGRLLHKTVIVQQSNPETGKPVSIPFFGPYTTVNGRIWPYADVDDGWYRLRLVNASNARIYNLVLIDEDDRPVPGVVHQIGSDGGLLPRPVPVDFDDTLPVLSAAPAERFDLLVDFRALGGRRLRLVDKGPGAPAGTPDPLGGVRYPEVMEFRVRETCEEDSFALPEVLSGSFRRMSHDIPHGHRLIVLTPPGTKGSGGHPEIWEMAEVEDPADVQVPAEGVIQVTGADGRTKTYRRTAATFNDGLGFTIGEGTHEQWTFLNLSPILHPMHIHLADFQVLGRDAYDASGFDLALGGTRTPVRLDPDTPVPLAPNELGHKDVFQVPGPQGLRVMGKFDGAYGRFMYHCHLLEHEDMGMMRPFVVMPPEALKFDHGGAHGGHGEGHTG