Function
Catalyzes the hydroxylation of 3-hydroxyphenylacetate and 3,4-dihydroxyphenylacetate to 2,5-dihydroxyphenylacetate (homogentisate) and 2,4,5-trihydroxyphenylacetate, respectively. Both of these compounds are used as substrate by homogentisate dioxygenase in the homogentisate pathway. The homogentisate pathway is used to catabolize phenylacetate and use it as a carbon source. Can also catalyze the hydroxylation of phenylacetate to 2-hydroxyphenylacetate at low efficiency to compensate for loss of phacA.
Sequence
MAIAEALLFVNNRAVEHPLQFLTAVAFAVPLLYVVINEFIRASARIPGFKGPRGLPLIGNLAQIRKDAAEQYRIWSKIYGPVYQIQLGNIPVLVVNSAAAAKVLFGQNAQALSSRPEFYTFHKIVSNTAGTTIGTSPYSESLKRRRKGAASALNRPSVESYVHHLDVESKAFVAELFKYGNGGKTPVDPMAMIQRLSLSLALTLNWGVRVASQEEELFDEITEVEDKISKFRSTTGNLQDYIPILRLNPFSSNSHKAKEMRDRRDKYLSSLNRDLDDRMEKGTHKPCIQANVILDKEAKLNSEELTSISLTMLSGGLDTVTTLVAWSISLLAQRPDIQDKAAKAIQEMYSEGQPLCDPADDQKCAYVAALVRECLRYYTVLRLALPRTSIRDITYDGKVIPKGTVFFLNAWACNMDPEVWSDPDEFRPERWFEQPDAPMFTYGMGYRMCAGSLLANRELYLVFIRTLNSFRIEPTEEKIEWHPLKGNSDPTSLVAIPKKYKVRFVPKNEVSLVEALS