pcn3

Gene

pcn3

Protein

DNA polymerase sliding clamp 3

Organism

Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)

Length

249 amino acids

Function

Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.

Similarity

Belongs to the PCNA family.

Mass

27.935 kDa

Sequence

MADARFYFSDARTWRYMVASIEKIIEEGVFVATGEGLSLRALDTSHVAMVDLYYPNTAFIEYDIGGESVEFGVSFDLLSKVLRRARKEDELVLEVEGSRLAVKLKSRGERTFRIPQVVMTYEKLPEPKVSFTVRARMLGSTFREAVRDLEPHSETLTLRALEDALLLVGSSEMATVEIELSQSRGSLLDYEAESQDRASYSIEYFSEMLSAAQAADAVVVSFSEDAPVRVDMEYLGGGRLTFYVSPKIE

Protein

DNA polymerase sliding clamp 3: pcn3

Gene

pcn3

Protein

DNA polymerase sliding clamp 3

Organism

Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)

Length

246 amino acids

Function

Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication (By similarity). Both trimeric complexes inhibit DNA ligase and both 3'-5' and 5'-3' activity of Hel308 (Hjm) helicase, but stimulate Hjc, the Holliday junction cleavage enzyme.

Similarity

Belongs to the PCNA family.

Mass

27.437 kDa

Sequence

MRVKVIDADAFSYIFRTLEEFIDEITLDFTSDGLKIRGIDPSRVTFIDILIPAGYFEEYNVEKEEKVGVKLEDFTDVLKTVTKNDSLYLETDENQNIKVTLDGVYERTFTFPSIVASEIETPNLNLEFPFKAKALTVTFTDIIDEIEDIGGDSITFKAEGGKLYLSANSDMGSSTIELSTENGGLLESEGGDAESVYGLEYVVNTSKMRKPSDTVEIAFGSQIPLKLRYNLPQGGYADFYIAPRAE

Protein

DNA polymerase sliding clamp 3: pcn3

Gene

pcn3

Protein

DNA polymerase sliding clamp 3

Organism

Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Length

244 amino acids

Function

One of the sliding clamp subunits that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation. Heterotrimer stimulates the Holliday junction resolvase Hjc.

Similarity

Belongs to the PCNA family.

Mass

27.459 kDa

Sequence

MKVVYDDVRVLKDIIQALARLVDEAVLKFKQDSVELVALDRAHISLISVNLPREMFKEYDVNDEFKFGFNTQYLMKILKVAKRKEAIEIASESPDSVIINIIGSTNREFNVRNLEVSEQEIPEINLQFDISATISSDGFKSAISEVSTVTDNVVVEGHEDRILIKAEGESEVEVEFSKDTGGLQDLEFSKESKNSYSAEYLDDVLSLTKLSDYVKISFGNQKPLQLFFNMEGGGKVTYLLAPKV

Protein

DNA polymerase sliding clamp 3: pcn3